The human brain tissue bank is an institution dedicated to the development of neurology and neuroscience through collection, preservation, research and sharing of donated human brain tissues. In order to maintain environment and staff personal safety, it is necessary to establish standard operation procedure for prevention of potential contagious diseases. During autopsy or brain only autopsy, it is necessary to evaluate the biological safety grades beforehand, prepare for potential risks, adhere to the requirement of corresponding levels of biological safety, and establish biological safety system. Following standard biosafety procedure is vital for human brain and tissue banking. On the basis of consultation, literary and regulation searches, and our own experiences, this paper summarizes the biosafety standards and procedures for human brain and tissue banking as guidelines during practice.