Research progress on the effect of electroacupuncture treatment on Alzheimer's disease model mice

Fei XU; Wangjun TANG; Mengying HONG

doi:10.3969/j.issn.2096-5516.2024.04.014

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Chinese Journal of Alzheimer's Disease and Related Disorders ›› 2024, Vol. 7 ›› Issue (4) : 317-321. DOI: 10.3969/j.issn.2096-5516.2024.04.014

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Research progress on the effect of electroacupuncture treatment on Alzheimer's disease model mice

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Abstract

Alzheimer's disease is a neurodegenerative disease with insidious onset, and Electroacupuncture is one way to treat Alzheimer's disease. Therefore, this article intends to summarize the experimental research on electroacupuncture treatment of Alzheimer's disease in mice in recent years, in order to provide reference for further exploring the pathogenesis of electroacupuncture treatment of Alzheimer's disease.

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Electroacupuncture / Alzheimer's disease / Mechanism / Research progress

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Fei XU , Wangjun TANG , Mengying HONG. Research progress on the effect of electroacupuncture treatment on Alzheimer's disease model mice[J]. Chinese Journal of Alzheimer's Disease and Related Disorders. 2024, 7(4): 317-321 https://doi.org/10.3969/j.issn.2096-5516.2024.04.014

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https://doi.org/10.13880/j.cnki.65-1174/n.2023.22.015

Cited in this article [1] Abstract

目的探讨枸杞多糖(LBP)对阿尔茨海默病(AD)合并2型糖尿病(T2DM)小鼠学习记忆能力及脑内Tau蛋白磷酸化水平的影响。方法 5月龄C57BL/6J小鼠16只随机分为对照组、T2DM组,同月龄APP/PS1小鼠32只随机分为AD组、AD+T2DM组、LBP组(100 mg·kg<sup>-1</sup>)、多奈哌齐组(0.75 mg·kg<sup>-1</sup>),每组各8只。T2DM组、AD+T2DM组、LBP组、多奈哌齐组小鼠高糖高脂饲料联合腹腔注射链脲佐菌素建立T2DM模型。治疗组以相应剂量药物干预,其余组给予等体积生理盐水。连续给药3个月后,Morris水迷宫实验评价各组小鼠学习记忆能力,HE染色观察各组小鼠脑组织细胞形态变化,Western Blot检测各组小鼠脑内不同位点磷酸化Tau蛋白及GSK3β蛋白的表达水平。结果 LBP可缩短AD+T2DM模型小鼠逃避潜伏期、第一次穿越平台时间(P<0.05,P<0.05),增加穿越平台次数及目标象限停留时间(P<0.01,P<0.01),改善大脑皮层神经元形态损伤,降低皮层及海马Tau蛋白Ser404、Ser396及Ser199位点磷酸化水平(P<0.05,P<0.05,P<0.05;P>0.05,P<0.01,P<0.01)和GSK-3β及Tyr216位点磷酸化水平(P>0.05,P<0.01;P<0.01,P<0.01),提高GSK-3β蛋白Ser9位点磷酸化水平(P<0.01,P<0.05)。结论枸杞多糖可改善AD+T2DM模型小鼠的学习记忆能力,降低脑内Tau蛋白的磷酸化水平。

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https://www.ncbi.nlm.nih.gov/pubmed/32881772

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Acupuncture is widely used to treat various neurodegenerative diseases and can effectively improve cognitive and memory states in Alzheimer's disease. However, its mechanism is unclear. We speculated that the effect of acupuncture on cognitive function may be associated with reductions in the levels of Aβ and phosphorylated tau in the brain. In this experiment, 60 male Sprague-Dawley rats were randomly divided into control, model, electroacupuncture and nonacupoint groups. We perform electroacupuncture at Shenting (GV24) and bilateral Benshen (GB13) acupoints once a day for 4 weeks in electroacupuncture group (with 1 day of rest after every 6 days of treatment). The electroacupuncture group showed a better performance in cognitive-related behavior tests and significantly lowers the levels of Aβ, p-tau (s396) and p-tau (s404) in the hippocampus. These results may suggest that electroacupuncture at the GV24 and bilateral GB13 acupoints might improve cognitive functions in Alzheimer's disease by decreasing the levels of Aβ, p-tau (s396) and p-tau (s404) in the brain as these proteins are the main causes of neurological damage and cognitive dysfunction during the pathogenesis underlying Alzheimer's disease.

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