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PDF(1271 KB)
PDF(1271 KB)
一种改进型麦克马斯特虫卵计数板的应用效果研究
Application Evaluation of a Modified McMaster Egg Counting Plate
为了评价一种改进型麦克马斯特虫卵计数板的性能,利用改进型麦克马斯特虫卵计数板和经典麦克马斯特虫卵计数板对兔粪便样品和球虫卵囊悬液样品进行计数和比较分析。结果显示,用饱和氯化钠溶液作为漂浮液,改进型麦克马斯特虫卵计数板能对卵囊悬液样品和2~5 g固体粪便样品中球虫卵囊进行准确计数,与麦克马斯特虫卵计数板计数结果的符合率为95.59%~99.49%;用自来水作为悬浮液,改进型麦克马斯特虫卵计数板能对卵囊悬液样品和0.3~0.5 g固体粪便样品中球虫卵囊进行准确计数。本研究发现,改进型麦克马斯特虫卵计数板对临床和实验室样品中虫卵能进行准确计数,而且比经典麦克马斯特虫卵计数板具有更强大的检测功能。
To evaluate the performance of modified McMaster egg counting plate developed in our lab, fecal samples of rabbits and oocyst suspension of Eimeria tenella were examined and counted using both classic and modified McMaster egg counting plates. The results of floating methods with saturated sodium chloride solution showed that the numbers of oocysts in rabbit fecal samples (2-5 g) and oocyst suspension were precisely counted using the modified McMaster plate. The coincidence rate with the results of classic McMaster plate was 95.59%-99.49%. In the precipitation experiment with tap water, the oocyst numbers in rabbit fecal samples (0.3-0.5 g) and oocyst suspension were also precisely counted using the modified McMaster plate. These findings suggest that the modified McMaster egg counting plate could be used to count the oocyst or egg number in fecal specimen and suspensions in labs, and is more powerful in counting functions than the classic McMaster plate.
改进型麦克马斯特虫卵计数板 / 粪样 / 虫卵悬液 / 虫卵 / 卵囊 / 包囊 / 定量分析
modified McMaster egg counting plate / fecal samples / egg suspension / eggs / oocysts / cysts / quantitative analysis
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The present study was aimed to evaluate the influence of flotation solution, sample dilution, and the choice of McMaster slide area (volume) on the reliability of the McMaster technique in estimating the faecal egg counts of gastrointestinal (GI) strongyles and Dicrocoelium dendriticum in a composite sample of faeces from naturally infected sheep. Fourteen flotation solutions having densities between 1.200 and 1.450, and six sample dilutions, 1:10, 1:15, 1:20, 1:30, 1:40 and 1:50 were used. Each of the six dilutions was divided into 70 aliquots in order to have five replicates of each of the 14 flotation solutions at each of the six dilutions. For each McMaster slide, the GI strongyle and D. dendriticum egg counts were performed under one grid (McM 0.15 ml), two grids (McM 0.3 ml), one chamber (McM 0.5 ml), and both chambers (McM 1.0 ml). Mean eggs per gram (EPG) of faeces of GI strongyles and D. dendriticum were calculated and statistical analyses were performed on the resulting data. The type of flotation solution used significantly influenced the EPG in the GI strongyles and in the D. dendriticum egg counts. All the sucrose-based solutions at density between 1.200 and 1.350 floated more GI strongyle eggs than the others. With respect to D. dendriticum, only six solutions were capable of floating eggs and the potassium iodomercurate solution (density 1.440) floated more eggs than the others. The reliability of the McMaster technique regarding sample dilution was high for both GI strongyle and D. dendriticum EPG at 1:10 and 1:15, and then progressively decreased with increasing dilution. The reliability of the McMaster technique regarding the choice of the McMaster slide area (volume) was high for both GI strongyle and D. dendriticum EPG at the McMaster slide area (volume) of 1.0 ml, i.e. the total area of the McMaster slide. The EPG counts resulting from choosing any of the other three McMaster slide areas (volumes), i.e. McM 0.15 ml, McM 0.3 ml, or McM 0.5 ml, produced unreliable over-estimates. The findings of the present study show that the highest reliability of the McMaster technique for estimating GI strongyle and D. dendriticum egg counts in faeces from pastured sheep is obtained when using flotation solutions based on sucrose for GI strongyles, and potassium iodomercurate for D. dendriticum, dilutions which do not exceed 1:15, and the McMaster slide area (volume) of 1.0 ml.
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