The wheat have different standard on the constituent of protein、gluten、zeleny sedimentation volume, ect, for different use. The yield and quality of three varieties of wheat were studied in 9 different water and fertilizer treatment. The results show that there are significant difference in quality and yield of different genotype wheat. The yield and quality of wheat were mostly determined by the genotype, and water or fertilizer have significant effect on yield and grains quality. The variation of grains quality and yield of different genotype wheat are different to the water and fertilizer treatment.