Abbreviation (ISO4): Chinese Journal of Alzheimer's Disease and Related Disorders
Editor in chief: Jun WANG
Chinese Journal of Alzheimer's Disease and Related Disorders >
Study on the behavior and blood index of TAUT transgenic rats
Received date: 2021-05-08
Revised date: 2021-06-12
Online published: 2021-09-25
Objective: To study the changes of cognitive ability and blood parameters in a brain-specific transgenic rat model of taurine transporter (TAUT) (TauT-tg rats). Methods: TauT-tg rats and negative rats (NON-transgenic, NTG rats) were selected. The morris water maze test was used to detect their learning and cognitive ability, and heart blood was collected to detect the changes in total protein, albumin, and alkaline phosphatase. As well as the changes of 30 blood routine indexes such as white blood cell count, red blood cell count, hemoglobin and platelets, etc. The brain tissues to the cortex and hippocampus were observed under electron microscope. Results: Compared with NTG rats, there is no significant difference in the over all positioning navigation latency of TauT-tg rats, while the proportion of TauT-tg male rats in the platform quadrant is significantly shorter on the 5th day. The total protein, Albumin of TauT-tg female rats are lower than that of NTG female rats. There is no difference in alkaline phosphatase,while the indicators of male rats are not significantly different, the blood routine index of TauT-tg and NTG rats are not significant sexual differences, but within the same group, the white blood cell count and red blood cell count of male rats were significantly higher than that of female rats, and there was no significant difference in hemoglobin, platelet and other indicators. Analyzing the ultrastructure of neurons and organelles in TauT-tg rats by electron microscopy showed that there was no significant difference between TauT-tg and NTG rats. Conclusion: Transgenic TAUT may improve the cognitive ability of male rats and has no effect on general blood indicators of rats, but may cause malnutrition in female rats.
Key words: Taurine transporter; rat; behavior; blood index
MAO Li-dong , FAN Wei-jia , LI Xu , WANG Chen , QI Hao-ming , TONG Hui-hui , XIA Yi-ming , HUANG Xiao-ling , ZHANG Ling , CHEN He-hong , HUANG Hui-ling . Study on the behavior and blood index of TAUT transgenic rats[J]. Chinese Journal of Alzheimer's Disease and Related Disorders, 2021 , 4(3) : 195 -200 . DOI: 10.3969/j.issn.2096-5516.2021.03.005
图2 TauT-tg大鼠和NTG大鼠的水迷宫实验:2A雌雄大鼠的定位航行潜伏期;2B、2C雄性大鼠、雌雄大鼠定位航行目标象限时间占比率;2D、2E雄性大鼠、雌雄大鼠定位航行目标象限距离占比率;2F雌雄大鼠空间探索达到平台时间;2G 雌雄大鼠空间探索穿越平台区次数。2H 、2 I NTG大鼠/TauT-tg大鼠 第1天和第5天的定位航行轨迹; 2J、2K 、NTG大鼠/TauT-tg大鼠第5天的空间探索轨迹。Fig.2 Morris Water Maze of two groups(TauT-tg and NTG rats): 2A Escaping latency of place navigation ; 2B 2C The proportion of time in the target quadrant of place navigation ; 2D 2E The proportion of distance in the target quadrant of place navigation ; 2F The time to reach the palteform of spatial probe test ;2G The number of crossings platform area of spatial probe test; 2H 2 I Swimming track on the 5th day of place navigation in NTG and TauT-tg rats ; 2J 2K Swimming track on the 1th and 5th day of spatial probe test in NTG and TauT-tg rats. |
表1 TauT-tg大鼠和对应NTG大鼠血项生化指标比较(x±s)Tab. 1 Comparison of biochemical indexes between sex in TauT-tg and NTG rats (x±s) |
检测项目 | 单位 | TauT-tg 大鼠 | NTG 大鼠 | t值 | |||
---|---|---|---|---|---|---|---|
雄性(n=7) ♂ | 雌性(n=6) ♀ | 雄 性 (n=7) ♂ | 雌性(n=9) ♀ | 雄性♂ | 雌性♀ | ||
总蛋白(TP) | g/L | 60.11±2.32 | 61.53±3.00① | 62.45±4.47 | 67.41±6.03 | 0.242 | 0.04① |
白蛋白(ALB) | g/L | 28.62±1.55 | 29.63±1.41① | 29.99±2.23 | 33.72±3.02 | 0.211 | 0.009① |
碱性磷酸酶(ALP) | U/L | 110.28±30.55 | 87.00±20.53 | 126.28±38.84 | 89.40±35.37 | 0.409 | 0.884 |
Note: ①P<0.05, comparison between two groups of the same sex. |
表2 TauT-tg大鼠与对应NTG大鼠的血液常规指标结果(x±s)Tab. 2 Multiple comparison of blood routine indexes of TauT-tg and NTG rats (x±s) |
检测项目(单位) | TauT-tg 大鼠 | NTG大鼠 | ||
---|---|---|---|---|
雄 性(n=7) | 雌性(n=6) | 雄 性 (n=9) | 雌性(n=10) | |
白细胞计数(×109/L) | 8.03±3.80 | 5.05±2.25① | 6.85±3.42 | 4.21±1.84① |
红细胞计数(×1012/L) | 10.14±0.65 | 8.48±1.21① | 10.27±0.32 | 8.81±1.18① |
血小板计数(×109/L) | 1158.57±190.86 | 1115.40±203.81 | 1191.78±115.66 | 1089.64±165.94 |
血红蛋白浓度 (g/L) | 170.85±9.67 | 150.25±20.61 | 169.55±8.60 | 159.92±20.86 |
Note: ①P<0.05, comparison between male and female in the same group. |
表3 TauT-tg大鼠不同时段血涂片棘红细胞比率与人血涂片比较(x±s)Tab. 3 Comparison of the ratio of acanthocytes at different periods of TauT-tg rats and human in the blood smear test (x±s) |
时间 | TauT-tg大鼠棘红细胞比率(%) | 人棘红细胞比率(%) | P值 |
---|---|---|---|
0.5h | 1.30±0.50 | 0.80±0.20 | 0.097 |
1.5h | 35.40±4.80 | 0.90±0.20 | 0.001 ② |
3h | 86.00±3.40 | 1.00±0.20 | 0.001 ② |
P值 | 0.001① | 0.12 |
Notes: ①P<0.05, Comparison between different time; ②P<0.01, comparison between rat and human. |
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