Performance Study of the RTyper Y27 Paternity Detection Kit on Two Detection Platforms

Boxu CHEN; Daoyu WANG; Zhenjun JIA; Bin ZHUANG; Lijian ZHAO; Yiren YAO; Junping HAN

doi:10.16605/j.cnki.1007-7847.2024.07.0161

Life Sci Res ›› 2025, Vol. 29 ›› Issue (5) : 413-418. DOI: 10.16605/j.cnki.1007-7847.2024.07.0161

TECHNOLOGY AND APPLICATION

Performance Study of the RTyper Y27 Paternity Detection Kit on Two Detection Platforms

Boxu CHEN ¹ ,
Daoyu WANG ¹ ,
Zhenjun JIA ¹^,^* ,
Bin ZHUANG ² ,
Lijian ZHAO ² ,
Yiren YAO ³ ,
Junping HAN ⁴^,^*

Author information +

History +

Abstract

To verify the performance of RTyper Y27 paternity detection reagent on both the traditional poly-merase chain reaction-capillary electrophoresis (PCR-CE) platform and the domestic Quick TargSeq^TM 1.0 in-tegrated system for rapid forensic DNA analysis, the RTyper Y27 paternity detection kit was used on both detection platforms, and 649 samples were examined for genetic polymorphism, loci detection rate, concor-dance rate, and simulated case detection. The detection results from 546 samples on PCR-CE platform demonstrated that the gene diversity (GD) values of this RTyper Y27 paternity detection reagent were not less than 0.43, with the highest value (0.88) at DYS449 among the 25 gene loci. Both the detection rate and con-cordance rate of 546 samples were 100.00%. The loci detection rate and the concordance rate of 100 samples on the Quick TargSeq^TM 1.0 integrated system platform were 96.37% and 99.64%, respectively. Furthermore, both the loci detection rate and concordance rate of three repeated detections on the simulated case were 100.00%. The results demonstrated that the RTyper Y27 paternity detection kit exhibited high gene polymor-phism with accurate and reliable genotyping, supporting rapid integrated testing on domestic Quick TargSeq^TM 1.0 system platform.

Key words

forensic genetics / rapid DNA genotyping / Y-chromosomal short tandem repeat (Y-STR) / perfor-mance detection

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Boxu CHEN , Daoyu WANG , Zhenjun JIA , et al . Performance Study of the RTyper Y27 Paternity Detection Kit on Two Detection Platforms[J]. Life Science Research. 2025, 29(5): 413-418 https://doi.org/10.16605/j.cnki.1007-7847.2024.07.0161

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