To verify the performance of RTyper Y27 paternity detection reagent on both the traditional poly-merase chain reaction-capillary electrophoresis (PCR-CE) platform and the domestic Quick TargSeq^TM 1.0 in-tegrated system for rapid forensic DNA analysis, the RTyper Y27 paternity detection kit was used on both detection platforms, and 649 samples were examined for genetic polymorphism, loci detection rate, concor-dance rate, and simulated case detection. The detection results from 546 samples on PCR-CE platform demonstrated that the gene diversity (GD) values of this RTyper Y27 paternity detection reagent were not less than 0.43, with the highest value (0.88) at DYS449 among the 25 gene loci. Both the detection rate and con-cordance rate of 546 samples were 100.00%. The loci detection rate and the concordance rate of 100 samples on the Quick TargSeq^TM 1.0 integrated system platform were 96.37% and 99.64%, respectively. Furthermore, both the loci detection rate and concordance rate of three repeated detections on the simulated case were 100.00%. The results demonstrated that the RTyper Y27 paternity detection kit exhibited high gene polymor-phism with accurate and reliable genotyping, supporting rapid integrated testing on domestic Quick TargSeq^TM 1.0 system platform.