Lachnoclostridium Mediates the Association Between Dietary Legume Intake and Blood Lipid Levels

Guang LI; Yun ZHANG; Maoxin REN; Qiong ZHANG; Yanjun DENG; Yuhao CUI; Jiamei HUANG; Weiyi YANG; Xiaobao WANG; Hao FENG; Yixuan XU; Sumei XIAO

doi:10.16605/j.cnki.1007-7847.2025.02.0119

Life Sci Res ›› 2025, Vol. 29 ›› Issue (5) : 389-397. DOI: 10.16605/j.cnki.1007-7847.2025.02.0119

BASIC MEDICINE

Lachnoclostridium Mediates the Association Between Dietary Legume Intake and Blood Lipid Levels

Author information +

History +

Abstract

This study aimed to investigate the relationship between dietary legume intake and blood lipid levels in adults with dyslipidemia or borderline elevated lipid levels, and to assess the mediating effect of gut microbes in this association. From May 2023 to May 2024, a total of 436 community-dwelling residents in Guangzhou, China, with dyslipidemia or borderline elevated lipid levels, were recruited. Differences in gut microbiota were determined using 16S rRNA high-throughput sequencing. The relationship between dietary legume intake and blood lipid levels was analyzed using multiple linear regression. The dietary legume in-take related genera in gut microbes were detected using microbiome multivariable linear models, and the mediating effects of gut microbes were assessed by mediation analysis. The results showed that, after adjusting for the confounders, dietary legume intake was significantly negatively associated with total cholesterol (β= -0.010, P<0.050) and low-density lipoprotein cholesterol (β=-0.012, P<0.050). A total of 21 genera were found to be significantly associated with dietary legume intake (q<0.250). Among them, Lachnoclostridium exhibited a significant mediating effect on the negative association between dietary legume intake and low-density lipoprotein cholesterol levels (P=0.048). This study provides a theoretical foundation for the effective manage-ment of lipid levels in individuals with dyslipidemia or borderline elevated lipid levels.

Key words

dietary legume intake / lipid level / gut microbe / Lachnoclostridium

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

Guang LI , Yun ZHANG , Maoxin REN , et al . Lachnoclostridium Mediates the Association Between Dietary Legume Intake and Blood Lipid Levels[J]. Life Science Research. 2025, 29(5): 389-397 https://doi.org/10.16605/j.cnki.1007-7847.2025.02.0119

References

List( Publishing order | Descend order by publishing year | Descend order by cited within ) Chart analysis

[1]	王增武, 刘静, 李建军, 等. 中国血脂管理指南(2023年)[J]. 中国循环杂志 (WANG Zengwu, LIU Jing, LI Jianjun, et al. Chi-nese guidelines for lipid management (2023)[J]. Chinese Circu-lation Journal), 2023, 38(3): 237-271. Cited in this article [4]

[2]	WANG W X, HU M L, LIU H, et al. Global burden of disease study 2019 suggests that metabolic risk factors are the leading drivers of the burden of ischemic heart disease[J]. Cell Meta-bolism, 2021, 33(10): 1943-1956.e2. Cited in this article [1]

[3]	ROTH G A, MENSAH G A, JOHNSON C O, et al. Global bur-den of cardiovascular diseases and risk factors, 1990-2019: up-date from the GBD 2019 study[J]. Journal of the American Col-lege of Cardiology, 2020, 76(25): 2982-3021. Cited in this article [1]

[4]	JING C L, WEN Z G, ZOU P, et al. Consumption of black legumes Glycine soja and Glycine max lowers serum lipids and alters the gut microbiome profile in mice fed a high-fat diet[J]. Journal of Agricultural and Food Chemistry, 2018, 66(28): 7367-7375. Cited in this article [2]

[5]	CHAMBERLIN M L, WILSON S M G, GASTON M E, et al. Twelve weeks of daily lentil consumption improves fasting cho-lesterol and postprandial glucose and inflammatory responses-a ran domized clinical trial[J]. Nutrients, 2024, 16(3): 419. Cited in this article [2]

[6]	NCHANJI E B, AGEYO O C. Do common beans (Phaseolus vul-garis L.) promote good health in humans? A systematic review and meta-analysis of clinical and randomized controlled trials[J]. Nutrients, 2021, 13(11): 3701. Cited in this article [3]

[7]	JENKINS D J A, MIRRAHIMI A, SRICHAIKUL K, et al. Soy protein reduces serum cholesterol by both intrinsic and food displacement mechanisms[J]. The Journal of Nutrition, 2010, 140(12): 2302S-2311S. Cited in this article [2]

[8]	ROSS F C, PATANGIA D, GRIMAUD G, et al. The interplay between diet and the gut microbiome: implications for health and disease[J]. Nature Reviews Microbiology, 2024, 22(11): 671-686. Cited in this article [1]

[9]	FU J Y, BONDER M J, CENIT M C, et al. The gut microbiome contributes to a substantial proportion of the variation in blood lipids[J]. Circulation Research, 2015, 117(9): 817-824. Cited in this article [3]

[10]

YUE

C H

, TANG

, CHANG

M H

, et al. Dietary supplemen-tation with short- and long-chain structured lipids alleviates obesity via regulating hepatic lipid metabolism, inflammation and gut microbiota in high-fat-diet-induced obese mice[J]. Journal of the Science of Food and Agriculture, 2024, 104(9): 5089-5103.

Cited in this article [3]

[11]	GUO G J, WU Y L, LIU Y J, et al. Exploring the causal effects of the gut microbiome on serum lipid levels: a two-sample Mendelian randomization analysis[J]. Frontiers in Microbiology, 2023, 14: 1113334. Cited in this article [2]

[12]	YAO Z D, CAO Y N, PENG L X, et al. Coarse cereals and legume grains exert beneficial effects through their interaction with gut microbiota: a review[J]. Journal of Agricultural and Food Chemistry, 2021, 69(3): 861-877. Cited in this article [2]

[13]	ZHANG X T, IRAJIZAD E, HOFFMAN K L, et al. Modulating a prebiotic food source influences inflammation and immune-regulating gut microbes and metabolites: insights from the BE GONE trial[J]. EBioMedicine, 2023, 98: 104873. Cited in this article [2]

[14]

HOU

D Z

, ZHAO

Q Y

, YOUSAF

, et al. A comparison be-tween whole mung bean and decorticated mung bean: benefi-cial effects on the regulation of serum glucose and lipid disor-ders and the gut microbiota in high-fat diet and streptozo-tocin-induced prediabetic mice[J]. Food & Function, 2020, 11(6): 5525-5537.

Cited in this article [2]

[15]	CRAIG C L, MARSHALL A L, SJÖSTRÖM M, et al. Interna-tional physical activity questionnaire: 12-country reliability and validity[J]. Medicine and Science in Sports and Exercise, 2003, 35(8): 1381-1395. Cited in this article [1]

[16]	ZHANG C X, HO S C. Validity and reproducibility of a food frequency questionnaire among Chinese women in Guangdong Province[J]. Asia Pacific Journal of Clinical Nutrition, 2009, 18(2): 240-250. Cited in this article [1]

[17]	杨月欣. 中国食物成分表标准版[M]. 北京: 北京大学医学出版社 (YANG Yuexin. China Food Composition Tables: Standard Edition[M]. Beijing: Peking University Medical Press), 2018. Cited in this article [1]

[18]	THORISDOTTIR B, ARNESEN E K, BÄREBRING L, et al. Legume consumption in adults and risk of cardiovascular di-sease and type 2 diabetes: a systematic review and meta-analy-sis[J]. Food & Nutrition Research, 2023, 67: 9541. Cited in this article [1]

[19]	JOHN H S, DOUCET É, POWER K A. Dietary pulses as a means to improve the gut microbiome, inflammation, and appetite con-trol in obesity[J]. Obesity Reviews, 2023, 24(9): e13598. Cited in this article [1]

[20]	KADYAN S, SHARMA A, ARJMANDI B H, et al. Prebiotic po-tential of dietary beans and pulses and their resistant starch for aging-associated gut and metabolic health[J]. Nutrients, 2022, 14(9): 1726. Cited in this article [1]

[21]	LUTSIV T, WEIR T L, MCGINLEY J N, et al. Compositional changes of the high-fat diet-induced gut microbiota upon con-sumption of common pulses[J]. Nutrients, 2021, 13(11): 3992. Cited in this article [1]

[22]	WEXLER H M. Bacteroides: the good, the bad, and the nitty-gritty[J]. Clinical Microbiology Reviews, 2007, 20(4): 593-621. Cited in this article [1]

[23]	CHAROENSIDDHI S, CHANPUT W P, SAE-TAN S. Gut mi-crobiota modulation, anti-diabetic and anti-inflammatory pro-perties of polyphenol extract from mung bean seed coat (Vigna radiata L.)[J]. Nutrients, 2022, 14(11): 2275. Cited in this article [1]

[24]	YU M, LI Z M, RONG T, et al. Integrated metagenomics-me-tabolomics analysis reveals the cecal microbial composition, function, and metabolites of pigs fed diets with different starch sources[J]. Food Research International, 2022, 154: 110951. Cited in this article [1]

[25]	KELLY T N, BAZZANO L A, AJAMI N J, et al. Gut microbiome associates with lifetime cardiovascular disease risk profile a-mong bogalusa heart study participants[J]. Circulation Research, 2016, 119(8): 956-964. Cited in this article [1]

[26]	CHEN P, CHEN X Q, HAO L L, et al. The bioavailability of soybean polysaccharides and their metabolites on gut microbiota in the simulator of the human intestinal microbial ecosystem (SHIME)[J]. Food Chemistry, 2021, 362: 130233. Cited in this article [2]

[27]	FLUITMAN K S, DAVIDS M, OLOFSSON L E, et al. Gut mi-crobial characteristics in poor appetite and undernutrition: a cohort of older adults and microbiota transfer in germ-free mice[J]. Journal of Cachexia, Sarcopenia and Muscle, 2022, 13(4): 2188-2201. Cited in this article [1]

[28]	MURALIDHARAN J, ROMAIN C, CHUNG L, et al. Effect of Sinetrol®Xpur on metabolic health and adiposity by interactions with gut microbiota: a randomized, open label, dose-response clinical trial[J]. Nutrition & Metabolism, 2024, 21(1): 83. Cited in this article [1]

[29]

CUI

W X

, MA

, LI

X P

, et al. Structural rearrangement of na-tive and processed pea starches following simulated digestion in vitro and fermentation characteristics of their resistant starch residues using human fecal inoculum[J]. International Journal of Biological Macromolecules, 2021, 172: 490-502.

Cited in this article [1]

[30]	JIANG S J, YU C, LV B J, et al. Two-sample Mendelian ran-domization to study the causal association between gut micro-biota and atherosclerosis[J]. Frontiers in Immunology, 2023, 14: 1282072. Cited in this article [1]

[31]	PANG W Q, WANG D, ZUO Z H, et al. Kidney bean fermented broth alleviates hyperlipidemic by regulating serum metabolites and gut microbiota composition[J]. Nutrients, 2022, 14(15): 3202. Cited in this article [1]