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Optimizing Metabolic Pathways by Using Bioretrosynthesis Tools
Liu Fufeng, Liu Xuzhi, Li Jinbi, Lu Fuping
Prog Chem ›› 2024, Vol. 36 ›› Issue (4) : 501-510.
PDF(420770 KB)
PDF(420770 KB)
Optimizing Metabolic Pathways by Using Bioretrosynthesis Tools
Biocatalysis has become an important technology In the field of biosynthesis because of its mild reaction conditions,high efficiency,high specificity and low price.There are a series of highly integrated metabolic networks in the biosynthesis system,and the study of multi-enzyme catalytic system has become an inevitable trend in the field of biosynthesis,so it is of great significance to explore the unknown multi-enzyme synthesis path based on the known products.in this review,the concepts of multi-enzyme system and retrosynthesis process are introduced.and the design methods,advantages and disadvantages of retrosynthesis tools are summarized.Then the tools are divided into host-based and host-less tools.For each of these two types,some representative retrosynthesis tools are listed to analyze their respective design processes and differences.Finally,the possibility of artificial intelligence-assisted multi-enzyme system is discussed and the optimization and development of multi-enzyme pathway construction tools are forecasted。
1 Introduction
2 Multienzyme catalysis
3 Methods for building retrosynthesis tools
4 Introduction to the retrosynthesis tools
4.1 Host-based retrosynthetic tools
4.2 Host-free retrosynthetic tools
5 Artificial intelligence fuels the development of multi-enzyme systems
6 Conclusion and outlook
multi-enzyme catalysis / path design / retrosynthesis / biological retrosynthesis tool
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