Abstract: The research is set up in order to get an efficient and acceptable system for stem tip germination of ‘Akihime’ strawberry. The system of rapid propagation was established by tissue culture of stem tip of ‘Akihime’ strawberry under different concentrations of exogenous hormones and different medium. The results showed that the best medium or treatment for various stages were as follows: (1) the larger the stem tips, the higher the survival rate. (2) Survival rate of stem tip was higher of 80% under the improved White medium and 40 g/L sucrose, pH 5.8. (3) Considering for both proliferation coefficient and quality, the acceptable proliferation medium was: MS6-BA 0.5 mg/LNAA 0.05 mg/L30 g/L saccharose, in this medium, the proliferation coefficient was 3.8. (4)Rooting medium: 1/2 MSsucrose 30 g/L. When using this acceptable system to germinate ‘Akihime’ strawberry, the research can get high efficiency.