To investigate the effects of different parents in interspecific hybridization of the genus Fraxinus on hybrid F₁ seeds, a total of 36 interspecific hybridization combinations of the genus Fraxinus were designed, and F₁ seeds from various hybrid combinations were collected. Statistical analysis of characteristic data such as thousand seed weight, wing length, wing width, and wing length/width of seeds was conducted. SPSS 24.0 software was used to perform variance analysis on the data, comparing the differences between inter species hybrids and intra species hybrids, as well as the effects of different genotypes of maternal and paternal parents on hybrid species. The results showed that there were significant differences in the four indicators of F₁ seeds between interspecific hybridization and intraspecific hybridization when F. pennsylvanica was used as the maternal parent and different species of Fraxinus male parent. The variation range between different combinations of interspecific hybridization was large. However, when using F. velutina as the maternal parent and different species of Fraxinus male parent, there was no significant overall difference in seed traits between interspecific hybridization and intraspecific hybridization. Four genotypes of F. velutina were hybridized with the female parent of F. mandshurica, resulting in significant variations in seed traits in the F₁ generation. Five genotypes of F. velutina were hybridized with the male parent of F. pennsylvanica, resulting in significant differences in thousand seed weight traits in the F₁ generation; The F₁ seed trait variation obtained by crossing the male parent of F. mandshurica with and the female parent of F. velutina was superior to that of the hybrid combination with F. pennsylvanica as the female parent. The results showed that the F₁ hybrid seeds in the interspecific hybrid of the genus Fraxinus had abundant genetic variation. Overall, it is believed that the variation of hybrid seeds with F. velutina as the maternal parent is higher than that as the paternal parent. Different genotypes of F. mandshurica as the paternal parent have no significant effect on the seed traits of F₁ generation. The study provides a reference for the selection of parents for interspecific hybridization in the genus Fraxinus, further improving the efficiency of Fraxinus.

This study aims to provide scientific basis and practical guidance for the optimization and improvement of blueberry substrate cultivation in Yunnan Province. 12 blueberry substrate cultivation bases with the same planting variety and tree age in Yunnan Province were selected. Substrate samples were collected during the full fruit period, and indicators such as bulk density, EC value, pH, air porosity, water holding capacity, steam-water ratio, ≤1 mm weight, >1 mm weight, total nitrogen, available phosphorus, and available potassium were measured and evaluated using the membership function comprehensive analysis method. The results showed that there were extremely significant differences in various indicators of the substrates in different bases. The order of membership degree from large to small was Yanshan> Lanping> Mengzi> Shilin> Yulong> Chengjiang> Jingdong> Mile> Jianshui 2> Jianshui 1> Qilin> Qiubei. The Yanshan base had the highest comprehensive score, and its substrate ratio was coconut husk: peat: perlite= 2:1:1; the Lanping base was second, with a substrate ratio of coconut husk: peat: perlite= 4:3:3; the Mengzi base ranked third, with a substrate ratio of coconut husk: coconut block: perlite= 2:1:2. The research analysis found that the addition of peat improved the substrate performance, but there is relatively less high-quality peat on the market. It is recommended to prioritize coconut husk as the main component of the substrate in production and appropriately add perlite to improve the physical properties of the substrate. The substrate formula can be adjusted according to the actual situation to achieve the best cultivation effect.

In order to explore the morphological characteristics and germination characteristics of Rhododendron species, the morphological structures of seeds from eight species were observed using a stereo microscope (LIOO SZ745). We also explored the impacts of gibberellin (GA₃) concentration, GA₃ treatment time, and soaking time in water on the germination traits of Rhododendron species. Additionally, we conducted a comparative analysis of germination traits among different species and provenances of Rhododendron species, as well as the impact of seed size on germination. The results showed that: (1) the seeds of the Rhododendronspecies were spindle-shaped, oval and small, the longitudinal and the aspect ratio of R. micranthum seeds was significantly higher than the other three Rhododendron seeds, the transverse diameter of the R. molle seeds was significantly higher than the other three Rhododendron seeds (P<0.01). (2) R. micranthum had the highest germination rate at GA₃ 0 mg/L, 4 h in the processing time, and the soaking time of 24 h; R. simsii seed was the highest in germination rate at GA₃ 100 mg/L, the processing time was 12 h, and the soaking time was 48 h; when the GA₃ 400 mg/L, the processing time was 4 h, the soaking time was 24 h, the germination rate of the R. mucronulatum was the highest; when the GA₃ 0 mg/L, the processing time was 4 h, the soaking time was 48 h, the germination rate of R. molle was the highest. (3) There were differences in germination rate, germination potential, germination lag, germination peak, germination duration and germination index of Rhododendron seeds among different provenances. R. simsii had the highest germination rate and germination potential, R. mucronulatum had the longest germination lag, germination peak and germination duration, and R. molle had the largest germination index. (4) The longitudinal diameter, transverse diameter and aspect ratio of Rhododendron seeds were negatively correlated with germination rate, germination potential, germination time lag, germination peak period, germination duration and germination index. This study can provide a theoretical basis for species identification and introduction and propagation of Rhododendron plants.

The paper aims to investigate the effects of bumblebee pollination on the quality and economic benefits of fresh tomatoes. This study took the fresh tomato variety ‘Jingcai 8’ as the research object. Through relevant investigations, data analysis, and economic benefit calculations, the effects of bumblebee pollination and hormone dipping on fresh tomatoes overwintering cultivated in greenhouse were studied. The research results showed that bee pollination increased the fruit setting rate, single fruit weight, and total yield of fresh tomatoes. The rate of malformed fruits decreased by 78.05%, the number of single fruits increased by 91.52%, the total sugar content increased by 17.44%, the soluble solids increased by 7.89%, the sugar acid ratio increased by 14.07%, tomato sales revenue increased by 8.06%, cost savings were 11550 yuan/hm², efficiency increased by 38910 yuan/hm², and total benefits increased by 12.49%. In addition to economic benefits, bee pollination reduced the use of hormones and pesticides, which had good ecological benefits. This study provides a reference for the promotion and application of bee pollination technology in the production of fresh tomatoes.

5-Aminolevulinic acid (5-ALA) is widely present in animals, plants, and microbial cells and serves as a key precursor for tetrapyrrole compounds. It participates in the synthesis of essential metabolites such as heme, vitamin B₁₂, and chlorophyll, exhibiting diverse physiological functions. This paper summarized the biosynthesis pathway of 5-ALA and its transport mechanism across different cell types, analyzed the functions and regulatory mechanisms of important downstream metabolites derived from 5-ALA, and reviewed the current applications of 5-ALA in pharmaceutical and agricultural fields. Furthermore, this paper highlighted existing challenges, including immaturity of biosynthesis technology and lack of standardized application protocols, and provided future research prospects to guide its potential applications in agriculture.

Heavy metal contamination of soil affects soil and crop quality and poses a threat to human health. Traditional phytoremediation techniques face challenges such as prolonged remediation cycles, poor adaptability, and the complexity of pollution, thus making it crucial to explore the mechanisms of removal, decomposition, and detoxification through enhanced phytoremediation technologies in heavy metal-contaminated soils. By collecting literature on intensive phytoremediation of heavy metal-contaminated soils, we concisely described techniques such as genetic engineering, the application of plant growth regulators, microbial synergistic remediation, and the addition of chelating agents, focusing on how these techniques enhance plant tolerance to heavy metal ions and affect their transport within the plant. This paper proposed that future agricultural production should focus more on understanding the molecular mechanisms and gene regulatory networks of plants, as well as the demand and uptake capacity of different plants for specific nutrients. Additionally, it suggested exploring more in situ bioresistance resources and combinatorial modes, enriching the symbiotic systems of bacteria and plants within contamination ranges, optimizing the dosage of chelating agents, and prioritizing biodegradable chelating agents or developing environmentally friendly substitutes. These efforts aimed to provide a theoretical and practical basis for utilizing enhanced phytoremediation technologies to address soil heavy metal contamination.

Sulfuric acid digestion is an effective method to decompose cellulose, hemicellulose and lignin into monosaccharides or disaccharides. The experiment was designed as a 3-factor completely randomized experimental design with response surface method was used to investigate the effects of sulfuric acid mass fraction, acid digestion temperature and acid digestion time on the content of fulvic acid using rapeseed cake as the raw material for acid digestion, and to screen the optimal parameters for the preparation of fulvic acid from rapeseed cake. The results showed that the mass fraction of sulfuric acid, acid digestion temperature and acid digestion time had significant effects on the content of xanthohumic acid. The optimal parameters for the preparation of fulvic acid from rapeseed cake by sulfuric acid digestion were the temperature of 100℃, sulfuric acid mass fraction of 25% and acid digestion time of 0.5 h. The content of fulvic acid prepared from rapeseed cake by sulfuric acid digestion was the highest in this condition, which was 14.17%, nitrogen content was 0.56%, phosphorus content was 0.25% and potassium content was 0.0028%.

In order to gain a deeper understanding of the research status and future directions in the field of leisure agriculture, this paper took the relevant literature published in the CSSCI journals and Peking University core journals collected by CNKI from 2012 to 2023 as the data sources, and used CiteSpace software to analyze the dynamic trends of collaborating authors, collaborating institutions, research trends, and publication volume in this field. The results indicate that the majority of authors have rich connections among themselves, but some teams still have limited network connections. The research institutions are still mainly universities, and their connections withindustries are not close enough. The research topics focus on "rural tourism", "models", "industrial upgrading", etc., mainly focusing on 12 themes including leisure agriculture strategies, rural tourism, influencing factors, development difficulties, industrial integration, low-carbon transformation, and key areas. The related research shows the development trend from macro to micro, and from single discipline extension to interdisciplinary intersection. Future research can delve deeper into the diversity of research teams, the expansion of research content, and the innovation of research contexts. The conclusions can provide theoretical basis and practical guidance for comprehensively promoting rural revitalization.

In order to explore the differences in color value, SPAD value and quality of cured tobacco leaves with different maturity levels, the color parameters, SPAD values, conventional chemical composition and sensory quality in different sections of the tobacco leaves of three ripening levels were studied using the middle and upper tobacco leaves of ‘Yunyan 87’ as materials. The results showed that with the increase of the harvesting maturity of the middle and upper tobacco leaves, the L^*, a^* and b^* values of tobacco leaves gradually increased, the SPAD values gradually decreased, and the uniformity of leaf color deteriorated. The color value and SPAD value of different sections of the same tobacco leaf were different, and the color value of the section near the tip of the leaf was larger and the SPAD value was smaller. The color value of the lower section (C area) of the middle tobacco leaves and the upper section (D area) of the upper tobacco leaves was similar to the overall mean value of the leaves, and the SPAD value of the upper section (D area) of the middle and upper tobacco leaves was similar to the overall mean value of the leaves. From the perspective of the quality of cured tobacco leaves, with the improvement of maturity, the quality of tobacco leaves first increased and then decreased; the middle tobacco leaves were roasted when the yellow-green area of the leaf surface was about 60% and the main vein became white about 1/2, while the upper tobacco leaves were roasted when the yellow-green area of the leaf surface was about 80% and the main vein became white about 2/3, the comprehensive quality of the cured tobacco leaves was relatively good. In addition, the color parameters L^*, a^* and b^* of tobacco leaves were negatively correlated with SPAD values, and the color parameters and SPAD values were significantly or extremely significantly correlated with some conventional chemical composition indexes of tobacco leaves.

In this study, data on yield and quality of Zhaotong apples were obtained from China National Knowledge Internet (CNKI) full text database and Web of Science database (WOS), a linear model was established by fitting a regression model using least squares estimation in order to study the effects of climatic factors on yield and quality of Zhaotong apples. The coefficient of determination of annual rainfall was low for yield and soluble solids content, and was high for titratable acid and water contents. Mean annual temperature had lower coefficients of determination for single fruit weight, titratable acid content and fruit shape index, and had higher coefficients of determination for yield and soluble sugar content. In the Zhaotong apple growing area, the increase of annual rainfall and mean temperature decreased apple quality, while the increase of mean annual maximum temperature increased apple yield, and the increase of mean annual minimum temperature increased single fruit weight. Taking into account the current climatic factors and future trends, it is recommended to plant varieties tolerant to high temperatures and to irrigate 2-3 times from autumn to spring. In breeding and production management, it is recommended to focus on the selection of varieties with extended cold periods and late flowering and working out production programs.

To explore the curing process in heat pump bulk for one time harvesting upper tobacco leaves in the tobacco-rice multiple-cropping area, ‘Yunyan 87’ was used as the experimental material to study the effects of different curing processes on the ratio of greenish and miscellaneous tobacco, high quality tobacco ratio, average price, energy consumption, appearance quality, physical character, chemical composition, and smoking quality of the cured tobacco leaves. The results show that compared with the four-step curing process, the steady temperature and humidity drop curing process can enhance the chromaticity of tobacco leaves, increase the chlorogenic acid content of tobacco leaves, increase the proportion of main tobacco leaves by 5.61%, reduce the proportion of miscellaneous tobacco leaves by 38.05%, reduce the proportion of greenish tobacco leaves by 54.40%, increase the high quality tobacco ratio by14.74%, increase the average price, appearance quality index, and total score of smoking quality by 8.59%, 8.29%, and 2.38%, respectively, and reduce the total energy consumption cost by 3.95%. Therefore, for the one time harvesting upper tobacco leaves in the tobacco-rice multiple-cropping area, the use of steady temperature and humidity drop curing process in heat pump intensive curing barn can improve the quality of flue-cured tobacco leaves, reduce curing costs, and have certain promotion value.

In order to study the warming effect of different plastic film mulching methods on early spring corn in Wuhan, ensure safe sowing and increase yield per unit, a comparative observation experiment was conducted on three covering methods with ‘Etianyu No5’ as test material in Hannan District of Wuhan, including open field sowing, single-layer plastic film covering, and single-layer plastic film + small arch shed. The results showed that plastic film covering significantly increased the ground temperature by 10 cm, increased the effective accumulated temperature by 9.9%-22.1% compared to open field sowing, shortened the entire growth period by 5-13 days, and increased the seedling emergence rate by 20%-30%. Among them, the warming effect of plastic film + small arch shed treatment is the most significant, and the effect of promoting early market is the best; the open field sowing method is not suitable for promotion due to the high risk of low temperature and cold damage during the sowing period. Under the methods of plastic film covering and plastic film + arch shed, the average earliest suitable sowing period could be 8 days and 19 days earlier than open field sowing, respectively. In terms of selecting a safe sowing period, if continuous low temperature is forecasted, the germination rate could be improved by delaying sowing or adding small arch sheds, thereby reducing the risk of low temperature and cold damage.

In order to explore a new cultivation substrate and a simplified cultivation method for Coprinus comatus, using the conventional formula as the control, 30% waste exogenous nutrition bag of Morchella spp. was added instead of bran as the experiment formula, and the C. comatus was cultivated by simplified cultivation method. The mycelium growth, fruiting body yield, raw material cost and nutritional ingredient of C. comatus were analyzed. The results showed that the mycelium characters, fruiting body yield and characters of C. comatus cultivated with the experiment formula were not significantly different from control, and the cost of cultivation raw materials was reduced by 29%. The contents of protein, total amino acids, flavonoids, fat and ash were lower than those of the control. The waste exogenous nutrition bag of Morchella spp. can be used as substrate materials to provide nitrogen source and partial carbon source for the growth of C. comatus. The simplified cultivation method of C. comatus can be effectively simplify the cultivation process and reduce the cost of raw materials.

This study focused on four typical plantation types (Platycladus orientalis forest, Pinus tabuliformis- Amorpha fruticosa forest, Ziziphus jujuba forest, and Pinus tabuliformis- Rhus typhina forest) in the loess stone mountain area of northern Shaanxi. Their ecological benefits were comprehensively evaluated and a multi-dimensional ecological benefit evaluation system was constructed through community surveys, soil physicochemical property measurements, plant physiological index analyses, diversity index calculations, and using the entropy-weighted TOPSIS method. The results showed that the number of species in Platycladus orientalis forest and Pinus tabuliformis- A. fruticosa forest was 20, which was higher than that in Z. jujuba forest, and Pinus tabuliformis- R. typhina forest, while Pinus tabuliformis- R. typhina forest exhibited significantly higher Shannon-Wiener diversity index, Simpson dominance index, and Pielou evenness index in its tree and shrub layers than other 3 types of forest. However, there were no significant differences in herbaceous layer community characteristic indices among the 4 plantations. The total phosphorus content in the soil of Pinus tabuliformis- R. typhina forest was significantly higher than that in other 3 types of forest. The total potassium content in the soil of Platycladus orientalis forest and Pinus tabuliformis- R. typhina forest was significantly higher than that in the Pinus tabuliformis- A. fruticosa forest and Z. jujuba forest, while the available potassium content was significantly lower than that in the latter 2 forests. The leaves photosynthetic capacity of the broadleaf trees (Z. jujuba and R. typhina) was significantly higher than that of the coniferous tree (Pinus tabuliformis and Platycladus orientalis), while the leaf water content was significantly lower than that of the coniferous tree. Comprehensive analysis revealed that soil physicochemical properties and the physiological state of dominant tree species significantly influenced understory vegetation community composition. The comprehensive ecological benefit evaluation ranked the four plantations in descending order as follows: Pinus tabuliformis- R. typhina forest, Pinus tabuliformis- A. fruticosa forest, Z. jujuba forest, and Platycladus orientalis forest. The study confirmed that the coniferous and broad-leaved mixed forest improved community stability and soil nutrient supply capacity through niche complementarity, which was more suitable for ecological restoration along the loess stone mountain area in northern Shaanxi, and the mixed forest of Pinus tabuliformis and R. typhina could be promoted as a preferred model.

This study explores the genetic basis and related functional genes of rice nitrogen use efficiency (NUE), highlighting the overuse of nitrogen fertilizers and summarizing the pertinent genes. NUE traits are divided into physiological traits (such as nitrogen uptake) and agronomic traits (such as tiller number) to elucidate key genes beneficial for nitrogen absorption. In physiological traits, genes affecting the absorption of NH₄⁺ and NO₃^- in rice are summarized. The absorption of NH₄⁺ in rice is primarily controlled by the AMT superfamily of proteins, whereas the proteins involved in NO₃^- transport mainly fall into two categories: NRT1 and NRT2, such as overexpression of genes like OsAMT1.1 and OsNRT1.1B can increase rice's nitrogen uptake. In agronomic traits, transcription factors such as GRF4 and NGR5 regulate the expression of nitrogen metabolism genes, promoting tiller development and grain growth, thus enhancing nitrogen use efficiency. These findings provide new genetic resources for rice breeding, promising the development of high NUE and environmentally friendly new varieties.

The purpose of this study is to make rational use of agricultural climate resources, carry out the climate suitable zoning of seabuckthorn planting in Aheqi, and arrange the planting area of seabuckthorn scientifically. Base on the historical meteorological data of 25 national meteorological stations in Aheqi County and its surrounding areas from 1991 to 2024, the climatic regionalization index of seabuckthorn planting in Aheqi County was determined by using ≥10°C accumulated temperature, frost-free period statistical method, grid mathematical model of spatial distribution of climatic elements and their changes, and agricultural climatic regionalization method. By using ArcGIS technology, the seabuckthorn planting area was divided into four areas : the most suitable area, the suitable area, the sub-suitable area and the unsuitable area. The research shows that the most suitable areas for planting sea buckthorn are in the middle and eastern part of the county, and the central and southern plains of the southern valley. The suitable areas are in the middle and eastern part of the county and the southern plain of the valley at an altitude of 1900-2100 m and 2200-2400 m. The sub-suitable planting areas are in the county valley plain and the valley hilly low mountain belt. The unsuitable areas are distributed in the middle and high mountain areas in the western, northern and southern mountainous areas of the county.

In response to the pressing challenges of land resource scarcity, climate change, and environmental pollution, this study designs an AIoT-based smart soilless cultivation system that integrates soilless cultivation with modern information technology. The system aims to overcome the limitations of traditional agriculture and promote the development of agricultural automation, intelligence, and precision management. This system incorporates Internet of Things (IoT) sensing, computer vision, big data analytics, and machine learning technologies. It employs multi-source heterogeneous data fusion to analyze crop growth conditions, utilizes artificial intelligence algorithms for intelligent greenhouse environment regulation, and enables remote visual monitoring through web and mobile interfaces. The entire system is highly integrated in terms of hardware and software on the Jetson nano platform, offering excellent parallel computing capabilities and scalability. Experimental results demonstrate that, compared to the manually managed control group, vegetables under the intelligent regulation exhibited a 15.4% shorter growth cycle, a 17.0% increase in plant height, a 26.7% increase in leaf count, and a 27.4% improvement in plant weight. Additionally, the remote control interface proved to be convenient and efficient, validating the system's outstanding performance in promoting the modernization of soilless agricultural cultivation. This system provides robust technical support for the development of precision agriculture and has the potential to drive modern agriculture towards sustainable, efficient, and environmentally friendly development.

Cultivated land serves as the material foundation for human survival and development. The construction of high-standard farmland is a crucial measure to enhance grain production capacity and ensure national food security. The evaluation of cultivated land quality grades is an important means to improve land management and protection intensity. This study employs the concept of fuzzy mathematics, utilizing methods such as the membership function method and expert experience method to standardize the evaluation factors. It investigates and evaluates the quality of cultivated land before and after the high-standard farmland construction project in four townships of Yuanping City in 2021. The results showed that the content of organic matter, available phosphorus and available potassium in the project area increased by 7.45%, 9.59% and 6.48%, respectively. The average grade of cultivated land quality after construction was 5.73, which was 0.54 higher than that before construction. To a certain extent, the high-standard farmland construction project has enhanced the quality level of cultivated land and the grain production capacity in Yuanping City.

The paper aims to address the issues of wheat variety selection and corresponding pest and disease control in different regions. Firstly, a data corpus with 3481 wheat variety data and 312 pest data was constructed. According to the characteristics of the corpus, the knowledge system model layer of wheat variety and pest control was defined in a fine grained manner. Secondly, the comparative advantages of Bert and Word2vec word - embedding models on small and medium-sized datasets were verified on public datasets and constructed datasets respectively, and then the attribute knowledge of wheat varieties and pests was extracted by using models suitable for each scale datasets. The experimental results showed that the F₁ value of Bert-BiLSTM-CRF model was 0.1499 higher than that of Word2vec-BiLSTM-CRF model in breed datasets. Word2vec-BiLSTM-CRF model was superior to Bert-BiLSTM-CRF model in pest datasets.

Through a bibliometric analysis of literature on the development of smart agriculture in China, this study aims to understand the publication status, research hotspots, emerging trends, and development directions within this field. Based on data collected from CNKI, the study retrieved and analyzed 94 articles published over the past five years (2019-2023) in core journals indexed in NU, CSSCI, and CSCD under the theme of "smart agriculture development." The results revealed the following: (1) publications on smart agriculture began to surge in 2019, reaching a peak in 2021; (2) universities and research institutions served as the primary centers for research on smart agriculture development; (3) most Chinese scholars focused their research on aspects such as development logic, development models, strategic frameworks, bidirectional mechanisms, and optimization paths within smart agriculture development; (4) the majority of research achievements in this field had been supported by project funding. Research on smart agriculture development is currently in a growth phase, with universities and research institutes contributing the largest volume of publications. In future, the development of smart agriculture should be explored from more novel perspectives in alignment with national strategic requirements.