This study compared different methods and parameters for extracting earthworm fibrinolytic enzyme (EFE) from Eisenia fetida, analyzed the enzyme properties, and promoted the development and utilization of earthworm processing byproducts. The extraction process of EFE was studied by combining fibrin plate enzyme activity assay, dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and high performance liquid chromatography-mass spectrometry (HPLC-MS). The optimal extraction process was as follows: using dry earthworm powder as raw material, extract with 5 times the mass ratio of 10 mmol/L Tris-HCl buffer, centrifuge twice and combine the supernatants; precipitate the crude extract with 80% ammonium sulfate, concentrate with a membrane separation method with a pore size of 10k-200 kD, and limit the membrane circulation filtration to ≤50 min; freeze-dry without adding a protective agent. Under this extraction and drying process, the enzyme activity of the freeze-dried powder was 201.8 million U/g. Enzyme characterization studies revealed that the enzyme comprised six earthworm-specific proteins with molecular weights ranging from 24.7 kD to 26.3 kD. The optimal pH range for the enzyme solution is 7.2 to 8.0, with a relatively stable pH range of 7.4 to 8.8. The optimal temperature for the enzyme solution is below 50℃, and it remains stable below 60℃. After 80 days of storage at 4℃, the enzyme activity retention rate can reach 90%. The ammonium sulfate-freeze drying method can extract enzymatically stable lumbrokinase products from dried E. fetida powder. The experimental results provide a reference for the high utilization of earthworms.

In order to study the impact of maximum residue limit standards for agricultural and veterinary drugs on China honey export trade, this article provides an overview of the current agricultural and veterinary drug residue limit regulations and standards in China, the European Union, the United States, Japan, and Australia, as well as the maximum residue limit standards in honey. It also compares and analyzes the relevant limit regulations and standards in China with the aforementioned countries and regions. As a result, China only has limited regulations on 6 pesticides and veterinary drugs in honey in national standards, whereas the European Union, Japan, Australia, and the United States have respectively established limits for 538, 79, 4, and 3 pesticides and veterinary drugs in their respective national standards and regulations. China industry standards have 13 MRL standards for pesticides and veterinary drugs, which have revised and supplemented the National Standard to some extent. However, compared with the standard systems of developed countries, standards for the limit of pesticide and veterinary drug residues in honey are relatively weak in China. Furthermore，There are differences in the types and quantities of MRL standards for agricultural and veterinary drugs in honey between China and other countries and regions such as the European Union, but China's standards are in line with its actual national conditions. In order to avoid the obstruction of honey export, we can refer to the standards of other countries and regions with China honey trade, and provide technical support for the revision of MRL standards for agricultural and veterinary drugs in China.

In order to accurately evaluate the harvest maturity of blueberries and ensure fruit quality, the experiment selected six blueberry cultivars: ‘Northland’, ‘Bluecrop’, ‘Draper’, ‘Reka’, ‘Duke’, and ‘Blue gold’ as test materials, and used near-infrared spectroscopy to establish regression model to determine the relationship between soluble solids, titratable acids, hardness, anthocyanins, vitamin C, and absorbance difference index (I_AD value). Research had found that with fruit maturity increase, the titratable acid and hardness of blueberries significantly decreased, while the contents of soluble solids, vitamin C, and anthocyanins generally showed an upward trend. The I_AD value of blueberries showed a highly significant positive correlation with anthocyanins (r=0.90, p≤0.01) and a significant negative correlation with hardness (r=-0.82, p≤0.01). Additionally, there was a positive correlation with vitamin C and soluble solids, and a negative correlation with titratable acid. Regression equations were derived to describe the changes in the I_AD value based on the five quality indicators, demonstrating that the I_AD value can reflect the fruit maturity. The recommended harvest standards for the six main cultivars are as follows. The I_AD values of ‘Northland’ and ‘Lanjin’ are 1.9-2.0, the I_AD values of ‘Bluecrop’ and ‘Draper’ are approximately 1.8, and the I_AD values of ‘Reka’ and ‘Duke’ are 1.7-1.8.

In order to study the effects of meteorological conditions on the internal quality and appearance quality of sunflower seed kernels in Hetao Irrigation District, using the staged sowing experiment to record the development period of sunflowers, and the contents of linoleic acid and oleic acid in sunflower kernels were tested. The incidence of sunflower kernel rust spots, disease index and sunflower borer feeding rate were counted. Using Excel, the numerical relationship between the internal and external quality of sunflower kernel and meteorological conditions was analyzed. The results showed that the sowing date had obvious influence on the inner quality and appearance of sunflower. Considering these two aspects, the best sowing date should be from May 25 to June 2. When the sunshine duration from flowering to maturity was 393.3-396.4.8 h, the content of linoleic acid could be greatly increased and the content of oleic acid could be reduced. When the average relative humidity from flowering to maturity was 58%-59%, the quality of sunflower could be significantly improved. When the average temperature from flowering to maturity was 19.0-20.8℃, the inner quality and appearance of sunflower were obviously improved. Precipitation from flowering to maturity had significant effects on sunflower quality. When precipitation was 42-44 mm, the content of linoleic acid in sunflower was greatly increased. When the precipitation was 46.9 mm, the gnawing rate of moth was the lowest. Precipitation, relative humidity and water vapor pressure at flowering stage were negatively correlated with the occurrence index of sunflower rust spot. With the increase of moisture and humidity, the number of drought-fond pest thrips decreased sharply, so the incidence index of rust spot decreased.

As the basis for plant growth, soil quality directly determines the success or failure of landscaping in urban areas. To understand the impact of conditioners on soil quality, we selected typical relocation site soil as the research object, and studied the impact on physical and chemical properties of the soil under different conditioners, different application methods and different time. The results showed that after applying different conditioners, each conditioner could effectively reduce the pH and increase the EC value of the relocated soil within a period of time, while the organic matter and total nitrogen of the soil had a tendency of decreasing to a certain extent, among which the decrease of Fe-S treatment was the most significant, while other treatments were not obvious; the impact of each conditioner on soil physical properties did not demonstrate clear improvement, which was mainly observed in the reduction of total soil porosity, non-capillary porosity and capillary porosity, increase of soil bulk density, decrease of soil infiltration rate, maximum water holding capacity and capillary water holding capacity. And among these treatments, M-S showed relatively poorer results; each treatment reduced the seed germination index, but the difference was not obvious, and the Fe-I treatment had poorer results than other treatments. The application of conditioner can improve the chemical properties of the soil in urban relocation site to a certain extent, but it is not obvious to improve the physical properties of the soil. Therefore, to achieve the desired effect of improving both the physical and chemical properties of the soil, it is suggested that the conditioner should be mixed with organic amendment materials.

In order to screen out the superior individual in the hybrid seedlings of poplars, and then select the excellent clones, we analyzed the genetic variation of the two-year-old seedlings of poplars using the cutting cross technology. The results showed that the growth, branch and leaf traits of different crossing combinations and different individuals of the same crossing combination had considerable genetic variation. Their family heritabilities were all above 0.81, which belonged to high heritability, indicating that the variation among cross-combinations was controlled by genetic factor. The results of multiple comparison showed that (P. alba× P. glandulosa) × P. bolleana × (P. tomentosa × P. bolleana) × P. canescens, (P. alba× P. gnaludotas ‘1’) × (P. tomentosa× P. bolleana) × P. canescens, (P. alba× P. glandulosa) × P. bolleana × (P. tomentosa× P. bolleana) × P. canescens had fast growth. Among the three cross combinations, (P. alba× P. glandulosa)× P. bolleana, P. alba× P. gnaludotas ‘1’, (P. alba× P. glandulosa)× P. bolleana were used as female parents and (P. tomentosa× P. bolleana)× P. canescens was used as male parent, the four parents were regarded as excellent germplasm resources. 20 seedlings were selected as superior individual plants, the average enrolment rate was 4.22%. 3-104×MY and YX1×MY were selected as superior cross combinations, which laid a foundation for the selection of excellent clones.

To study the characteristics of pedogenesis and types attribution of soils in Yongnian District of Handan City, the 3^rd National Soil Survey was carried out in the district in 2022. A total of 616 surface soil samples and 20 soil profiles were collected, 6 typical soil profiles were selected as the study subjects, and the position of each soil body in the Keys to Chinese Soil Taxonomy (CST) was determined through the analysis of soil profile morphological characteristics and physical and chemical properties. According to the classification search results of CST, it was found that the diagnostic layers in the test profile included ochric epipedon, cambic horizon, and argic horizon, and the diagnostic features included redoxic features, calcaric property, lithologic characters, soil temperature regimes, and soil moisture regimes. In CST, the studied soils were belonged to 3 orders of primosols, argosols, and cambosols, 4 suborders, 4 groups and 4 subgroups; meanwhile, 5 soil families and 6 soil series (Zhaili series, Jiyao series, etc.) were established of the selected soil profiles. The reference between pedogenesis and system classification of 6 soils profiles was conducted from high and base taxon. It provides a precedent for carrying out the soil survey and soil system classification work in Handan City.

Determining the safety threshold of heavy metals in soil is an important measure to ensure the quality and safety of agricultural products in China. By studying the accumulation and transportation characteristics of five heavy metals (Cr, Cd, Pb, As, and Hg) in different organs of corn and wheat in the cities of Jinchang, Zhangye and Baiyin in Gansu Province and establishing the relationship between the effective content of Cr, Cd, Pb, As, and Hg in soil and crop grains, this study identified the safety threshold of effective heavy metals in soil for corn and wheat systems. This study employed the method and principles of species sensitivity distribution (SSD) and derived the safety threshold of effective heavy metals in soil for corn and wheat planting systems based on the cumulative probability distribution curve of the Logistic function distribution model. The results showed that the accumulation pattern of Cr, Cd, Pb, As and Hg in wheat plants was consistent, namely, roots>stems>grains, and similarly, the accumulation pattern of Cr, Pb, As and Hg in corn plants was roots>stems>grains, while the accumulation pattern of Cd was stems>roots>grains. Utilizing the Logistic function distribution model to fit the cumulative probability distribution curve based on the effective content of heavy metals, the safety thresholds of effective Cr, Cd, Pb, As and Hg in wheat soil were determined to be 0.019, 0.771, 35.294, 2.777 and 0.133 mg/kg, respectively. Meanwhile, the safety thresholds of effective Cr, Cd, Pb, As and Hg in corn soil were determined to be 0.296, 7.90, 52.363, 12.462 and 0.119 mg/kg, respectively. The results of this study indicate that estimating the safe threshold of effective heavy metal content based on the cumulative probability distribution curve method was scientific, providing a scientific basis and support for the safe planting and risk control of wheat and corn.

The study aimed to master the soil nutrients, soil environment, soil quality and soil environmental quality of green selenium-rich and germanium-rich production areas in Nanhua of central Yunnan. Based on the 630 surface soil samples data obtained from the 1:250000 land quality geochemical survey in Nanhua of central Yunnan, the soil nutrients, soil environment, soil quality and soil environmental quality of green selenium-rich and germanium-rich production areas in Nanhua County were evaluated according to relevant national or industry standards. The results showed that soil nutrient content in the study area was medium-highly rich, with 37.19% of the soil having sufficient nutrients of the first and second grades, and 42.35% of the soil having sufficient nutrients of the third grade. The overall soil environment was good, with first-grade soil (risk-free) accounting for 80.19%, second-grade soil (risk-controllable) accounting for 19.41%, and third-grade soil (higher risk) accounting for only 0.40%. The comprehensive grade of soil quality was generally high-quality and good, and the proportion of first-grade (high-quality) and second-grade (good) soil reached 60.26%. The proportion of soil that meets the AA grade green selenium-rich production area standard was 2.78%, and the area was 63 km². The proportion of soil that meets the AA grade green germanium-rich production area standard was 28.5%, and the area was 646 km². The proportion of soil that meets the AA grade green selenium-rich germanium production area standard was 2.78%, and the area was 63 km². The soil nutrients in the study area were relatively sufficient, the soil environment was generally good, and it had broad prospects for the development of green selenium-rich and germanium-rich foods. This study provided a scientific basis for the establishment of a green selenium-rich and germanium-rich planting and breeding experimental base and the development of green selenium-rich and germanium-rich agricultural and livestock products in central Yunnan, and contributed to the construction of ecological agriculture, local economic development and rural revitalization in Nanhua area, it also had a certain reference effect on the development of green agriculture and the land use planning in central Yunnan.

High-intensity land conversion and persistent land use activities exert dual effects on soil development, which can significantly influence pedodiversity. We first reviewed the influences of various types of land use on the source of parent materials, microclimate, microrelief, vegetation cover, management practices and soil age. Then based on our systematical analyses of the effects of land use change on soil physical, chemical, and biological properties, we summarized the effects of land conversions on soil genetic, morphological, and diagnostic characteristics. We also comprehensively evaluated soil type evolution trends in different classification categories in response to land conversions. Finally, we proposed three research priorities: (1) an overall understanding of the effect of diverse land use activities on the soil properties and formation processes; (2) deep exploration of dynamic soil genetic responses to land cover conversions and modifications; (3) and development of new technologies for exact and high-efficiency identification of soil type change with land use change.

To improve the quality of artificially cultivated licorice, a pot experiment was conducted at Yulin University using Ural licorice as the experimental material to analyze and evaluate its biomass, physiological and biochemical indicators, and root anatomical structure after adding exogenous silicon under drought stress. The results showed that the addition of exogenous silicon significantly increased the accumulation of dry matter in licorice under drought stress; the synthesis of glycyrrhetinic acid and glycyrrhizinwas significantly promoted; under drought stress, exogenous silicon addition increased root dry weight from 0.93 g/plant to 1.31 g/plant; the synthesis and accumulation of glycyrrhizin and glycyrrhetinic acidwere promoted, malondialdehyde contentwas reduced, leaf proline content and root soluble sugar contentwere increased, and xylem area/cross-sectional area and phloem/anatomical cross-sectional areawere increased; The addition of exogenous silicon significantly increased the hydraulic conductivity of licorice plants by 39.15% and 302% under two different soil moisture conditions, and the hydraulic conductivity and root injury volume of licorice plants were significantly positively correlated with licorice yield. The addition of exogenous silicon under drought stress improved the quality of licorice, promoted the synthesis and accumulation of secondary metabolites, enhanced root water absorption efficiency and overall hydraulic conductivity, and participated in the formation of root tissues under stress, thereby increasing licorice yield.

The study aims to master the natural precipitation patterns, water consumption and demand patterns of major crops, regional crop layout, and farmland water resource regulation in the Xing'an League region, which are important tasks for agricultural production and research. This study utilized meteorological data from the Zhalaite Banner National Science and Technology Demonstration Park in Xing'an League, Inner Mongolia, and used the FAO Penman Monteith formula and water balance method to investigate the water consumption patterns of major crops such as semi-arid corn, peanuts, and foxtail millet in Xing'an League, Inner Mongolia. Research indicated that among several major crops, soybean had the highest field water consumption and significant water deficit throughout their entire growth period, followed by corn. Peanuts and foxtail millet consumed less water in the field, resulting in relatively low water deficit. From the perspective of water use efficiency, the priority planting order of crops in the region should be corn > millet > peanut > soybean. From the perspective of water economy benefits, the order of priority crops planted in the region should be peanut > corn > foxtail millet > soybean; the priority crops for irrigation were peanut > foxtail millet > corn > soybean. Soybean planting should adopt the intercropping mode of corn and soybean, which had relatively high water use efficiency and water economic benefits. This study provides suggestions and recommendations for effectively adjusting regional crop layout and regulating agricultural water resources.

To analyze the correlation between plant species diversity and sampling area in different types of artificial lawn ecosystems, this study used a nested sampling method to track and investigate the plant species diversity of six commonly used artificial lawn ecosystems in China constructed by six different turf grasses. Two experimental treatments of manually removing weeds (MRW) and maintaining natural state (MNS) were set, and the changing patterns of plant species diversity with the increasing sampling area were analyzed. The results showed that the plant species diversity in the six different types of lawn ecosystems treated with MRW was significantly lower than that in the MNS experimental research plot, and the difference between these two experimental treatments became gradually greater as the sampling area increased, indicating that MRW management measures had a significant effect on maintaining the singularity of plant species and the evenness of plant growth in various types of lawn ecosystems. Both plant species diversity in the unit area and change rate of plant species diversity with the increased sampling area gradually decreased with the increase of construction years, indicating that the material cycle and energy flow inside the ecosystems tended to be stable, and the plant species composition and the functional structure of lawn ecosystems both were in the dynamic equilibrium states with the development and succession of lawn ecosystems. However, the maintenance mechanisms of this dynamic equilibrium state were different in the two experimental treatments of MRW and MNS, the former was that the regular artificial impurity removal management measure inhibited weed invasion and growth, while the latter was that the dominant plant species suppressed the growth and reproduction of the other types of plant species. The research results indicated that although various management measures had a strong intervention intensity on the lawn ecosystems, they still could not completely eliminate the continuous invasion and interference of various weeds; in the early stage of lawn construction, the ecosystem was unstable, and the frequency and intensity of management measures such as MRW should be increased; entering the mid- to late-stage, as the species composition and structural function of the lawn ecosystem tended to stabilize with the increase of construction years, the degree of manual intervention could be appropriately reduced, and the mutual constraint between the various components of the ecosystem could be fully utilized to manage the lawn for improving the quality of the lawn and reducing the amount of manual labor.

The aims were to study the growth and development law of Yutai rice under different accumulated temperature conditions, and to explore the influence of sunshine hours and temperature in different growth stages on the yield factors of Yutai rice, and to provide agricultural meteorological service basis for optimal planting of rice in Yutai area. A Logistic growth model was constructed based on the growth index data of Yutai rice from 2017 to 2022 and meteorological factors such as accumulated temperature during growth period. By using statistical methods such as correlation analysis and regression analysis, the influence of light and temperature in different growth stages on rice yield factors was analyzed, and the prediction model of yield factors was established accordingly. The results showed that the overall accuracy of Logistic model was high in the simulation of rice growth and development in Yutai, and the Root Mean Square Error (RMSE) between the simulated value and the measured value was between 0.591 and 5.100, the Normalized Root Mean Squared Error (nRMSE) was between 0.087 and 0.107, and the R² between the simulated value and the measured value was between 0.970 and 0.996. The number of sunshine hours in tillering, jointing, booting and grain filling maturity of rice was significantly correlated with yield, and the accumulated temperature in heading and grain filling maturity was significantly correlated with yield. The prediction model of rice yield and grain number per ear was established by multiple linear regression method, which was verified by historical band and histogram. The prediction model has high accuracy.

Based on the classical IPCC carbon emission calculation theory, the agricultural carbon emissions were calculated in Yunnan Province from 2005 to 2021, considering agricultural material input, soil nitrogen emissions, rice cultivation and livestock and poultry breeding. The influence factor decomposition was analyzed based on Logarithmic Mean Divisia Index. Results showed that: (1) the total agricultural carbon emissions in Yunnan Province tended to increase from 2005 to 2021, showing a relatively obvious three-stage change characteristic of “fluctuating decrease-continuous increase-fluctuating decrease”. The carbon emissions in 2021 increased by 22.38% compared to 2005, with an average annual growth rate of 1.27%. (2) In 2021, the source structure of agricultural carbon emissions ranked as livestock and poultry breeding, agricultural material input and crop farming, average accounting for 73.19%, 14.42% and 12.39% respectively. (3) The LMDI decomposition results showed that agricultural production efficiency, agricultural economic development level and urbanization were emission growth factors, while agricultural industrial structure, employment structure and rural population were emission reduction factors. Therefore, it is recommended to firmly promote the rapid development of urbanization, optimize the agricultural industry structure and employment structure, develop standardized scale breeding of livestock and poultry, and promote the modernization of agriculture and rural development.

Burdock root is rich in numerous functional active substances and exhibits functions such as lowering blood glucose, exerting antibacterial, anti-inflammatory, and antioxidant activities, regulating lipid metabolism, and modulating the intestinal flora. It is renowned as the ‘King of Vegetables’ and ‘Oriental Ginseng’. This study places emphasis on the types and physiological functions of active substances such as polysaccharides, polyphenols, dietary fibers, amino acids, fatty acids, volatile oils, lignans, phytosterols, and pentacyclic triterpenes in burdock root. It also summarizes the current status of the development and utilization of primary and advanced processing products of burdock root. On this basis, the development of the burdock root industry is prospected, with the aim of providing a reference for the application of burdock root in fields such as food, medicine, and the chemical industry, as well as for its high-quality industrialization.

Using blueberry ‘Eureka’ as experimental material, the effects of five light quality treatment combinations on blueberry fruit quality were studied to provide theoretical basis for scientific production. The results showed that, compared with the control, the single fruit weight, vertical and horizontal diameter, total anthocyanin and total flavonol content of treatment T2 (the light intensity is 28 μmol/(m²·s), and the spectrum is red: blue light=5:1, single power 27 W, length 1220 mm lamp is placed side by side at 30 cm at the top of blueberry plant) were the highest, which were higher than those of the control (no lamp tube), but the difference was not significant. The solid-acid ratio and sugar-acid ratio of treatment T₄ (the light intensity is 21 μmol/(m²·s) and the spectrum is red: blue light=1.4:1, single power 16 W, length 1080 mm lamp is placed side by side at the top 30 cm of blueberry plant) were significantly higher than those of the control, the content of total acid (citric acid, malic acid, shikimic acid) was significantly lower than that of the control, and the hardness and soluble solids content of treatment T₄ were the highest, higher than that of the control, but the difference was not significant. The total sugar content (glucose, fructose, sucrose) of treatment T₅ (The light intensity is 21 μmol/(m²·s), and the spectrum is red: blue light=1.4:1, single power 16 W, length 1080 mm, two rows side by side at the top 30 cm of the blueberry plant, and one row of lights at the bottom (along the blueberry basin). was the highest, which was significantly higher than that of the control. The comprehensive score of treatment T₄ was the highest. Treatment T₄ had the best effect on improving the fruit quality of blueberry, that is side by side at the distance from the 30 cm at the top of the blueberry plant, two rows of lamps with a light intensity of 21 μmol/(m²·s), and a spectrum of red light: blue light=1.4:1, single power 16 W, length 1080 mm.

In recent years, the economic benefits of root herbs have continued to rise, the scale of cultivation has been expanding year by year, leading to the rapid development of root herbs planting machinery. However, the planting machinery still has the problems of low planting efficiency, narrow application range, and high planting loss. To address these issues, scholars at home and abroad continue to innovate and optimize, and have achieved certain results. The current situation, development dynamics and constraints of domestic and foreign research on mechanized planting technology and equipment for root and tuber Chinese herbal medicines were reviewed in the article, the structure, their key technologies and types of representative domestic and foreign planting machinery were analyzed and summarized. And the shortcomings of domestic mechanized planting technology for root and tuber Chinese herbal medicines were pointed out, and corresponding countermeasures, suggestions and subsequent development trend and direction were put forward.

To investigate the effects of different parents in interspecific hybridization of the genus Fraxinus on hybrid F₁ seeds, a total of 36 interspecific hybridization combinations of the genus Fraxinus were designed, and F₁ seeds from various hybrid combinations were collected. Statistical analysis of characteristic data such as thousand seed weight, wing length, wing width, and wing length/width of seeds was conducted. SPSS 24.0 software was used to perform variance analysis on the data, comparing the differences between inter species hybrids and intra species hybrids, as well as the effects of different genotypes of maternal and paternal parents on hybrid species. The results showed that there were significant differences in the four indicators of F₁ seeds between interspecific hybridization and intraspecific hybridization when F. pennsylvanica was used as the maternal parent and different species of Fraxinus male parent. The variation range between different combinations of interspecific hybridization was large. However, when using F. velutina as the maternal parent and different species of Fraxinus male parent, there was no significant overall difference in seed traits between interspecific hybridization and intraspecific hybridization. Four genotypes of F. velutina were hybridized with the female parent of F. mandshurica, resulting in significant variations in seed traits in the F₁ generation. Five genotypes of F. velutina were hybridized with the male parent of F. pennsylvanica, resulting in significant differences in thousand seed weight traits in the F₁ generation; The F₁ seed trait variation obtained by crossing the male parent of F. mandshurica with and the female parent of F. velutina was superior to that of the hybrid combination with F. pennsylvanica as the female parent. The results showed that the F₁ hybrid seeds in the interspecific hybrid of the genus Fraxinus had abundant genetic variation. Overall, it is believed that the variation of hybrid seeds with F. velutina as the maternal parent is higher than that as the paternal parent. Different genotypes of F. mandshurica as the paternal parent have no significant effect on the seed traits of F₁ generation. The study provides a reference for the selection of parents for interspecific hybridization in the genus Fraxinus, further improving the efficiency of Fraxinus.

The study aims to investigate the impact of planting density on the yield and quality of the newly approved high-quality weak gluten wheat variety 'Yunmai 114' in Yunnan, providing a theoretical basis for determining a reasonable planting density for the large-scale promotion of weak gluten wheat varieties in Yunnan. Using 'Yunmai 114' as the experimental material, seven treatments including 900000 plants/hm² (A), 1.35 million plants/hm² (B), 1.8 million plants/hm² (C), 2.25 million plants/hm² (D), 2.7 million plants/hm² (E), 3.15 million plants/hm² (F), and 3.6 million plants/hm² (G) were set up for basic seedlings, with three replicates and incomplete random block arrangement. The yield and yield related traits, grain type traits, quality traits and stem tiller dynamics of the variety were investigated under different planting densities, and variance analysis and correlation analysis were conducted on these traits. The highest number of tillers, effective panicles, and the number of tillers in the third, fourth, fifth, sixth, and seventh leaf stages of 'Yunmai 114' showed extremely distinct differences at 7 planting densities. There were also significant differences in spike rate, grain number, plant height, water absorption rate, protein content, stable time, formation time, and hardness value at each planting density. However, there were no significant differences in theoretical yield, actual yield, thousand grain weight, grain area, grain circumference, grain length to width ratio, and grain length and width among different planting densities. The changes in both theoretical and actual yields showed a trend of first increasing, then decreasing, then increasing, and finally decreasing with the increase of planting density. There was a highly significant positive correlation (r=0.778**, 0.908**, 0.767**, 0.924**, 0.850**, and 0.752**) between the effective panicle, the number of tillers in the third, fourth, fifth, sixth, and seventh leaf stages of the variety and its basic seedlings. The plant height and theoretical yield were significantly positively correlated with their basic seedlings (r=0.536* and 0.520*). There was a significant negative correlation between the number of grains per panicle, protein content and stability time with their basic seedlings (r=-0.450*, -0.449*, and -0.467*), while there was a certain correlation but not significant between actual yield and 16 other traits with the basic seedlings. Planting density could significantly affect the number of tillers in the third, fourth, fifth, sixth, and seventh leaf stages, highest tiller number, effective panicle, spike rate, grain number, plant height, water absorption rate, protein content, stability time, formation time, and hardness value of 'Yunmai 114', but it did not significantly affect the theoretical yield, actual yield, thousand grain weight, grain area, grain circumference, and grain length to width ratio, grain length and grain width. Moreover, a low planting density was not conducive to the performance of the yield and quality characteristics of the variety. When the basic seedlings were 1.35 million -3.6 million plants/hm², the yield of the variety remained stable at 14389.50-16078.50 kg/hm², maintaining good yield and stability, as well as maintaining the quality characteristics of weak gluten wheat. These planting densities are suitable for promotion in areas with better water and fertilizer conditions in Yunnan.