In recent years, the betel nut planting industry in Hainan Province has developed rapidly. Industrial adjustment and optimization will be the focus of its future development, and a reasonable production area layout is crucial for the sustainable development of the betel nut industry in Hainan. Using regional comparative advantage index and resource endowment coefficient, this study analyzed the spatial-temporal evolution characteristics and driving factors of betel nut production in various cities and counties in Hainan from 2005 to 2022. The results showed that the scale of betel nut cultivation in Hainan had significantly increased, but the yield had significantly decreased. The calculated results of the comprehensive comparative advantage index and resource endowment coefficient showed strong consistency. The central regions of Qiongzhong, Ding'an, Baoting, and Tunchang had stronger comparative advantages in betel nut production, which were key areas for the production layout in Hainan. The leading advantage of traditional planting areas in the east was gradually weakening, while the overall western region lacked comparative advantages but showed a clear upward trend. The production of betel nuts in Hainan presents a pattern of retreating in the east, advancing in the west, and maintaining dominance in the center. The driving factors of spatial-temporal evolution mainly include higher economic benefits, the diffusion of high-yield cultivation and management techniques, and the spread of diseases and pests such as yellowing disease. It is recommended to reasonably adjust the layout of betel nut production, moderately control of planting scale, strengthen socialized services, clarify the regulatory policies for edible betel nut products, and accelerate the transformation and upgrading of the betel nut industry.

This study compared different methods and parameters for extracting earthworm fibrinolytic enzyme (EFE) from Eisenia fetida, analyzed the enzyme properties, and promoted the development and utilization of earthworm processing byproducts. The extraction process of EFE was studied by combining fibrin plate enzyme activity assay, dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and high performance liquid chromatography-mass spectrometry (HPLC-MS). The optimal extraction process was as follows: using dry earthworm powder as raw material, extract with 5 times the mass ratio of 10 mmol/L Tris-HCl buffer, centrifuge twice and combine the supernatants; precipitate the crude extract with 80% ammonium sulfate, concentrate with a membrane separation method with a pore size of 10k-200 kD, and limit the membrane circulation filtration to ≤50 min; freeze-dry without adding a protective agent. Under this extraction and drying process, the enzyme activity of the freeze-dried powder was 201.8 million U/g. Enzyme characterization studies revealed that the enzyme comprised six earthworm-specific proteins with molecular weights ranging from 24.7 kD to 26.3 kD. The optimal pH range for the enzyme solution is 7.2 to 8.0, with a relatively stable pH range of 7.4 to 8.8. The optimal temperature for the enzyme solution is below 50℃, and it remains stable below 60℃. After 80 days of storage at 4℃, the enzyme activity retention rate can reach 90%. The ammonium sulfate-freeze drying method can extract enzymatically stable lumbrokinase products from dried E. fetida powder. The experimental results provide a reference for the high utilization of earthworms.

In order to study the effects of meteorological conditions on the internal quality and appearance quality of sunflower seed kernels in Hetao Irrigation District, using the staged sowing experiment to record the development period of sunflowers, and the contents of linoleic acid and oleic acid in sunflower kernels were tested. The incidence of sunflower kernel rust spots, disease index and sunflower borer feeding rate were counted. Using Excel, the numerical relationship between the internal and external quality of sunflower kernel and meteorological conditions was analyzed. The results showed that the sowing date had obvious influence on the inner quality and appearance of sunflower. Considering these two aspects, the best sowing date should be from May 25 to June 2. When the sunshine duration from flowering to maturity was 393.3-396.4.8 h, the content of linoleic acid could be greatly increased and the content of oleic acid could be reduced. When the average relative humidity from flowering to maturity was 58%-59%, the quality of sunflower could be significantly improved. When the average temperature from flowering to maturity was 19.0-20.8℃, the inner quality and appearance of sunflower were obviously improved. Precipitation from flowering to maturity had significant effects on sunflower quality. When precipitation was 42-44 mm, the content of linoleic acid in sunflower was greatly increased. When the precipitation was 46.9 mm, the gnawing rate of moth was the lowest. Precipitation, relative humidity and water vapor pressure at flowering stage were negatively correlated with the occurrence index of sunflower rust spot. With the increase of moisture and humidity, the number of drought-fond pest thrips decreased sharply, so the incidence index of rust spot decreased.

In order to study the impact of maximum residue limit standards for agricultural and veterinary drugs on China honey export trade, this article provides an overview of the current agricultural and veterinary drug residue limit regulations and standards in China, the European Union, the United States, Japan, and Australia, as well as the maximum residue limit standards in honey. It also compares and analyzes the relevant limit regulations and standards in China with the aforementioned countries and regions. As a result, China only has limited regulations on 6 pesticides and veterinary drugs in honey in national standards, whereas the European Union, Japan, Australia, and the United States have respectively established limits for 538, 79, 4, and 3 pesticides and veterinary drugs in their respective national standards and regulations. China industry standards have 13 MRL standards for pesticides and veterinary drugs, which have revised and supplemented the National Standard to some extent. However, compared with the standard systems of developed countries, standards for the limit of pesticide and veterinary drug residues in honey are relatively weak in China. Furthermore，There are differences in the types and quantities of MRL standards for agricultural and veterinary drugs in honey between China and other countries and regions such as the European Union, but China's standards are in line with its actual national conditions. In order to avoid the obstruction of honey export, we can refer to the standards of other countries and regions with China honey trade, and provide technical support for the revision of MRL standards for agricultural and veterinary drugs in China.

Mikania micrantha is one of the most harmful weeds in tropical and subtropical regions, known as the "one-minute mile grass" and the title of "plant killer". With the increasing development of invasive alien plants, invasive alien plant Mikania micrantha has also become one of the hot spots of research and development at present. Transforming invasive plants into beneficial resources can not only avoid wasting a lot of manpower and material resources to control the spread of invasive plants, but also obtain product resources with better control effect. This paper summarizes the plant pesticides of Mikania micrantha in three aspects of crop pest control (fruit and vegetable crop pest, food crop pest, cash crop pest and other pests), crop virus, fungus and nematode control (pathogenic bacteria of food crop, pathogenic bacteria of vegetable crop, pathogenic bacteria of fruit, pathogenic bacteria of cash crop and nematode control) and other applications of agricultural control (herbicide, control of invasive alien organisms). Finally, the development prospect and future research direction of plant pesticides of Mikania micrantha were prospected.

In order to accurately evaluate the harvest maturity of blueberries and ensure fruit quality, the experiment selected six blueberry cultivars: ‘Northland’, ‘Bluecrop’, ‘Draper’, ‘Reka’, ‘Duke’, and ‘Blue gold’ as test materials, and used near-infrared spectroscopy to establish regression model to determine the relationship between soluble solids, titratable acids, hardness, anthocyanins, vitamin C, and absorbance difference index (I_AD value). Research had found that with fruit maturity increase, the titratable acid and hardness of blueberries significantly decreased, while the contents of soluble solids, vitamin C, and anthocyanins generally showed an upward trend. The I_AD value of blueberries showed a highly significant positive correlation with anthocyanins (r=0.90, p≤0.01) and a significant negative correlation with hardness (r=-0.82, p≤0.01). Additionally, there was a positive correlation with vitamin C and soluble solids, and a negative correlation with titratable acid. Regression equations were derived to describe the changes in the I_AD value based on the five quality indicators, demonstrating that the I_AD value can reflect the fruit maturity. The recommended harvest standards for the six main cultivars are as follows. The I_AD values of ‘Northland’ and ‘Lanjin’ are 1.9-2.0, the I_AD values of ‘Bluecrop’ and ‘Draper’ are approximately 1.8, and the I_AD values of ‘Reka’ and ‘Duke’ are 1.7-1.8.

In order to solve the inconvenience caused by the flying-catkins of kapok, different concentrations of “Yihua No.1” were carried out at the final flowering stage of kapok to study its influence on the formation and development of kapok fruit. Results showed that the application of “Yihua No.1” at the last flowering stage of kapok had a very significant effect on promoting abortion and fruit drop of kapok, and the higher the concentration of the drug, the more obvious the effect. A 3-year follow-up investigation showed that after the application of “Yihua No.1”, the sample plants appeared different degrees of trunk cracking, secreting gum, abnormal sprouting, dead branches, and weakened tree potential and so on. By the next year, the flower formation was inhibited, and some of them could not even complete flower bud differentiation. The higher the concentration, the more obvious such damage. Considering the effect of “Yihua No.1” and its adverse effects on trees, 0.5% concentration with 25 mg/cm, or 1% concentration with 12.5 mg/cm were recommended to use in production.

As the basis for plant growth, soil quality directly determines the success or failure of landscaping in urban areas. To understand the impact of conditioners on soil quality, we selected typical relocation site soil as the research object, and studied the impact on physical and chemical properties of the soil under different conditioners, different application methods and different time. The results showed that after applying different conditioners, each conditioner could effectively reduce the pH and increase the EC value of the relocated soil within a period of time, while the organic matter and total nitrogen of the soil had a tendency of decreasing to a certain extent, among which the decrease of Fe-S treatment was the most significant, while other treatments were not obvious; the impact of each conditioner on soil physical properties did not demonstrate clear improvement, which was mainly observed in the reduction of total soil porosity, non-capillary porosity and capillary porosity, increase of soil bulk density, decrease of soil infiltration rate, maximum water holding capacity and capillary water holding capacity. And among these treatments, M-S showed relatively poorer results; each treatment reduced the seed germination index, but the difference was not obvious, and the Fe-I treatment had poorer results than other treatments. The application of conditioner can improve the chemical properties of the soil in urban relocation site to a certain extent, but it is not obvious to improve the physical properties of the soil. Therefore, to achieve the desired effect of improving both the physical and chemical properties of the soil, it is suggested that the conditioner should be mixed with organic amendment materials.

This study, based on the land use change survey data from 2018, conducted a comprehensive analysis of the agricultural land consolidation potential in 113 administrative villages of Habahe County. Through GIS spatial analysis and hierarchical analysis methods, the potential of cultivated land was measured from two dimensions: quantity and quality. The research found that the area of agricultural land consolidation in Habahe County was 34482.41 hm², of which the existing cultivated land area was 30904.50 hm². After calculation, the quantitative potential of agricultural land consolidation could increase cultivated land by 2900.05 hm², with a new cultivated land coefficient of 8.41%. Combining the local crop planting structure system, it was calculated that agricultural land consolidation could increase cultivated land productivity by 3479.28 t. This indicated that Habahe County had significant potential for agricultural land consolidation. Based on the new cultivated land coefficient and the quality of cultivated land, the consolidation potential is divided into four levels. The research results point out that farmland construction in Habahe County should be supported by improving water and road infrastructure, strengthening farmland protection, and improving soil through agricultural biotechnological measures to enhance the quality of cultivated land, increase land use efficiency, and improve the output efficiency of cultivated land. The study provides a foundation and basis for future land remediation planning in this area.

In order to screen out the superior individual in the hybrid seedlings of poplars, and then select the excellent clones, we analyzed the genetic variation of the two-year-old seedlings of poplars using the cutting cross technology. The results showed that the growth, branch and leaf traits of different crossing combinations and different individuals of the same crossing combination had considerable genetic variation. Their family heritabilities were all above 0.81, which belonged to high heritability, indicating that the variation among cross-combinations was controlled by genetic factor. The results of multiple comparison showed that (P. alba× P. glandulosa) × P. bolleana × (P. tomentosa × P. bolleana) × P. canescens, (P. alba× P. gnaludotas ‘1’) × (P. tomentosa× P. bolleana) × P. canescens, (P. alba× P. glandulosa) × P. bolleana × (P. tomentosa× P. bolleana) × P. canescens had fast growth. Among the three cross combinations, (P. alba× P. glandulosa)× P. bolleana, P. alba× P. gnaludotas ‘1’, (P. alba× P. glandulosa)× P. bolleana were used as female parents and (P. tomentosa× P. bolleana)× P. canescens was used as male parent, the four parents were regarded as excellent germplasm resources. 20 seedlings were selected as superior individual plants, the average enrolment rate was 4.22%. 3-104×MY and YX1×MY were selected as superior cross combinations, which laid a foundation for the selection of excellent clones.

On the basis of quantitative analysis of China's formula feed standards, this article summarizes the evolution of China's formula feed standards in order to provide feasible suggestions for the development of the feed industry. This article applies quantitative analysis methods and selects national and non-national standard systems for formula feed of 39 years in China from 1984 to 2023 as research samples. ROST CM6 software is used to quantitatively analyze the evolution characteristics of formula feed standards in China. Research has found that China's feed standards have gone through four peak periods of standard formulation and publication, with a focus on feed standard testing, experimental animal formula feed, formula feed nutrition standards, and formula feed hygiene standards. Non national standards have a certain degree of advancement or lag compared to national standards. At different stages, China's formulated feed standards have been continuously revised and improved from promulgation to development into the current feed standard system with Chinese characteristics.

To improve the quality of seeds, realize the standardization of seeds, and promote the increase of rapeseed yield, the effects of different drug varieties on the growth of rapeseed were explored by pelletizing coating of rapeseed seeds in this study. Among them, the effects of pelletized seed coating agent (NXY-1) and Switzerland suspension seed coating agent (SLS) coated with different drug ratios on the seedling growth of rapeseed were studied using Brassica napus 'Fan-ming No. 1' as the test material. The results showed that the optimal seed mass ratio was 1:300 in the pelletized seed coating agent NXY-1, and the pelletized seed coating agent could promote seed emergence, with the highest dry-to-fresh weight ratio before winter and full flowering stage, and the nitrogen and phosphorus content and potassium content were the highest 21 days after emergence compared with the control, which could effectively increase the contents of chlorophyll and gibberellin, and the effect was better than that of the imported seed coating agent SLS.

To improve the quality of artificially cultivated licorice, a pot experiment was conducted at Yulin University using Ural licorice as the experimental material to analyze and evaluate its biomass, physiological and biochemical indicators, and root anatomical structure after adding exogenous silicon under drought stress. The results showed that the addition of exogenous silicon significantly increased the accumulation of dry matter in licorice under drought stress; the synthesis of glycyrrhetinic acid and glycyrrhizinwas significantly promoted; under drought stress, exogenous silicon addition increased root dry weight from 0.93 g/plant to 1.31 g/plant; the synthesis and accumulation of glycyrrhizin and glycyrrhetinic acidwere promoted, malondialdehyde contentwas reduced, leaf proline content and root soluble sugar contentwere increased, and xylem area/cross-sectional area and phloem/anatomical cross-sectional areawere increased; The addition of exogenous silicon significantly increased the hydraulic conductivity of licorice plants by 39.15% and 302% under two different soil moisture conditions, and the hydraulic conductivity and root injury volume of licorice plants were significantly positively correlated with licorice yield. The addition of exogenous silicon under drought stress improved the quality of licorice, promoted the synthesis and accumulation of secondary metabolites, enhanced root water absorption efficiency and overall hydraulic conductivity, and participated in the formation of root tissues under stress, thereby increasing licorice yield.

To study the characteristics of pedogenesis and types attribution of soils in Yongnian District of Handan City, the 3^rd National Soil Survey was carried out in the district in 2022. A total of 616 surface soil samples and 20 soil profiles were collected, 6 typical soil profiles were selected as the study subjects, and the position of each soil body in the Keys to Chinese Soil Taxonomy (CST) was determined through the analysis of soil profile morphological characteristics and physical and chemical properties. According to the classification search results of CST, it was found that the diagnostic layers in the test profile included ochric epipedon, cambic horizon, and argic horizon, and the diagnostic features included redoxic features, calcaric property, lithologic characters, soil temperature regimes, and soil moisture regimes. In CST, the studied soils were belonged to 3 orders of primosols, argosols, and cambosols, 4 suborders, 4 groups and 4 subgroups; meanwhile, 5 soil families and 6 soil series (Zhaili series, Jiyao series, etc.) were established of the selected soil profiles. The reference between pedogenesis and system classification of 6 soils profiles was conducted from high and base taxon. It provides a precedent for carrying out the soil survey and soil system classification work in Handan City.

Finding out the condition of soil quality is a prerequisite for ensuring national food security and developing smart agriculture. According to the characteristics of multi-point and wide distribution of farmers, it is necessary to provide regional distribution information of farmers in order to obtain soil quality information quickly and accurately. Based on the existing remote sensing inversion model of soil quality, this paper adopted ArcGIS Enterprise and other related software, and used Android mobile terminal as the platform, designed and developed soil quality (water, fertilizer, salinity and alkalinity) intelligent analysis APP for the needs of farmers. The three-layer structure of data layer, service layer and user layer was used to develop three functional modules of basic service, remote sensing inversion of soil quality and analysis and decision making, which could help farmers quickly and accurately grasp field soil quality information, and provide decision-making suggestions such as fertilization guidance and salinization treatment. The research results contributed to improving agricultural production efficiency, promoting the development of smart agriculture, which were of great significance for achieving agricultural modernization and information management.

Determining the safety threshold of heavy metals in soil is an important measure to ensure the quality and safety of agricultural products in China. By studying the accumulation and transportation characteristics of five heavy metals (Cr, Cd, Pb, As, and Hg) in different organs of corn and wheat in the cities of Jinchang, Zhangye and Baiyin in Gansu Province and establishing the relationship between the effective content of Cr, Cd, Pb, As, and Hg in soil and crop grains, this study identified the safety threshold of effective heavy metals in soil for corn and wheat systems. This study employed the method and principles of species sensitivity distribution (SSD) and derived the safety threshold of effective heavy metals in soil for corn and wheat planting systems based on the cumulative probability distribution curve of the Logistic function distribution model. The results showed that the accumulation pattern of Cr, Cd, Pb, As and Hg in wheat plants was consistent, namely, roots>stems>grains, and similarly, the accumulation pattern of Cr, Pb, As and Hg in corn plants was roots>stems>grains, while the accumulation pattern of Cd was stems>roots>grains. Utilizing the Logistic function distribution model to fit the cumulative probability distribution curve based on the effective content of heavy metals, the safety thresholds of effective Cr, Cd, Pb, As and Hg in wheat soil were determined to be 0.019, 0.771, 35.294, 2.777 and 0.133 mg/kg, respectively. Meanwhile, the safety thresholds of effective Cr, Cd, Pb, As and Hg in corn soil were determined to be 0.296, 7.90, 52.363, 12.462 and 0.119 mg/kg, respectively. The results of this study indicate that estimating the safe threshold of effective heavy metal content based on the cumulative probability distribution curve method was scientific, providing a scientific basis and support for the safe planting and risk control of wheat and corn.

The study aims to master the natural precipitation patterns, water consumption and demand patterns of major crops, regional crop layout, and farmland water resource regulation in the Xing'an League region, which are important tasks for agricultural production and research. This study utilized meteorological data from the Zhalaite Banner National Science and Technology Demonstration Park in Xing'an League, Inner Mongolia, and used the FAO Penman Monteith formula and water balance method to investigate the water consumption patterns of major crops such as semi-arid corn, peanuts, and foxtail millet in Xing'an League, Inner Mongolia. Research indicated that among several major crops, soybean had the highest field water consumption and significant water deficit throughout their entire growth period, followed by corn. Peanuts and foxtail millet consumed less water in the field, resulting in relatively low water deficit. From the perspective of water use efficiency, the priority planting order of crops in the region should be corn > millet > peanut > soybean. From the perspective of water economy benefits, the order of priority crops planted in the region should be peanut > corn > foxtail millet > soybean; the priority crops for irrigation were peanut > foxtail millet > corn > soybean. Soybean planting should adopt the intercropping mode of corn and soybean, which had relatively high water use efficiency and water economic benefits. This study provides suggestions and recommendations for effectively adjusting regional crop layout and regulating agricultural water resources.

The study aimed to master the soil nutrients, soil environment, soil quality and soil environmental quality of green selenium-rich and germanium-rich production areas in Nanhua of central Yunnan. Based on the 630 surface soil samples data obtained from the 1:250000 land quality geochemical survey in Nanhua of central Yunnan, the soil nutrients, soil environment, soil quality and soil environmental quality of green selenium-rich and germanium-rich production areas in Nanhua County were evaluated according to relevant national or industry standards. The results showed that soil nutrient content in the study area was medium-highly rich, with 37.19% of the soil having sufficient nutrients of the first and second grades, and 42.35% of the soil having sufficient nutrients of the third grade. The overall soil environment was good, with first-grade soil (risk-free) accounting for 80.19%, second-grade soil (risk-controllable) accounting for 19.41%, and third-grade soil (higher risk) accounting for only 0.40%. The comprehensive grade of soil quality was generally high-quality and good, and the proportion of first-grade (high-quality) and second-grade (good) soil reached 60.26%. The proportion of soil that meets the AA grade green selenium-rich production area standard was 2.78%, and the area was 63 km². The proportion of soil that meets the AA grade green germanium-rich production area standard was 28.5%, and the area was 646 km². The proportion of soil that meets the AA grade green selenium-rich germanium production area standard was 2.78%, and the area was 63 km². The soil nutrients in the study area were relatively sufficient, the soil environment was generally good, and it had broad prospects for the development of green selenium-rich and germanium-rich foods. This study provided a scientific basis for the establishment of a green selenium-rich and germanium-rich planting and breeding experimental base and the development of green selenium-rich and germanium-rich agricultural and livestock products in central Yunnan, and contributed to the construction of ecological agriculture, local economic development and rural revitalization in Nanhua area, it also had a certain reference effect on the development of green agriculture and the land use planning in central Yunnan.

To analyze the correlation between plant species diversity and sampling area in different types of artificial lawn ecosystems, this study used a nested sampling method to track and investigate the plant species diversity of six commonly used artificial lawn ecosystems in China constructed by six different turf grasses. Two experimental treatments of manually removing weeds (MRW) and maintaining natural state (MNS) were set, and the changing patterns of plant species diversity with the increasing sampling area were analyzed. The results showed that the plant species diversity in the six different types of lawn ecosystems treated with MRW was significantly lower than that in the MNS experimental research plot, and the difference between these two experimental treatments became gradually greater as the sampling area increased, indicating that MRW management measures had a significant effect on maintaining the singularity of plant species and the evenness of plant growth in various types of lawn ecosystems. Both plant species diversity in the unit area and change rate of plant species diversity with the increased sampling area gradually decreased with the increase of construction years, indicating that the material cycle and energy flow inside the ecosystems tended to be stable, and the plant species composition and the functional structure of lawn ecosystems both were in the dynamic equilibrium states with the development and succession of lawn ecosystems. However, the maintenance mechanisms of this dynamic equilibrium state were different in the two experimental treatments of MRW and MNS, the former was that the regular artificial impurity removal management measure inhibited weed invasion and growth, while the latter was that the dominant plant species suppressed the growth and reproduction of the other types of plant species. The research results indicated that although various management measures had a strong intervention intensity on the lawn ecosystems, they still could not completely eliminate the continuous invasion and interference of various weeds; in the early stage of lawn construction, the ecosystem was unstable, and the frequency and intensity of management measures such as MRW should be increased; entering the mid- to late-stage, as the species composition and structural function of the lawn ecosystem tended to stabilize with the increase of construction years, the degree of manual intervention could be appropriately reduced, and the mutual constraint between the various components of the ecosystem could be fully utilized to manage the lawn for improving the quality of the lawn and reducing the amount of manual labor.

The aims were to study the growth and development law of Yutai rice under different accumulated temperature conditions, and to explore the influence of sunshine hours and temperature in different growth stages on the yield factors of Yutai rice, and to provide agricultural meteorological service basis for optimal planting of rice in Yutai area. A Logistic growth model was constructed based on the growth index data of Yutai rice from 2017 to 2022 and meteorological factors such as accumulated temperature during growth period. By using statistical methods such as correlation analysis and regression analysis, the influence of light and temperature in different growth stages on rice yield factors was analyzed, and the prediction model of yield factors was established accordingly. The results showed that the overall accuracy of Logistic model was high in the simulation of rice growth and development in Yutai, and the Root Mean Square Error (RMSE) between the simulated value and the measured value was between 0.591 and 5.100, the Normalized Root Mean Squared Error (nRMSE) was between 0.087 and 0.107, and the R² between the simulated value and the measured value was between 0.970 and 0.996. The number of sunshine hours in tillering, jointing, booting and grain filling maturity of rice was significantly correlated with yield, and the accumulated temperature in heading and grain filling maturity was significantly correlated with yield. The prediction model of rice yield and grain number per ear was established by multiple linear regression method, which was verified by historical band and histogram. The prediction model has high accuracy.