Research Progress on Biocontrol Microorganisms Against Phytophthora nicotianae

HAO Haohao; ZHANG Yuwei; TANG Peipei; LI Xiang; WU Junlin; LIU Ziqi; MIAO Sen; LU Peng; JIA Wei; DANG Bingjun

doi:10.11923/j.issn.2095-4050.cjas2024-0041

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Journal of Agriculture ›› 2025, Vol. 15 ›› Issue (5) : 41-47. DOI: 10.11923/j.issn.2095-4050.cjas2024-0041

Research Progress on Biocontrol Microorganisms Against Phytophthora nicotianae

HAO Haohao ¹ ,
ZHANG Yuwei ² ,
TANG Peipei ¹ ,
LI Xiang ¹ ,
WU Junlin ¹ ,
LIU Ziqi ¹ ,
MIAO Sen ¹ ,
LU Peng ¹ ,
JIA Wei ² ,
DANG Bingjun ²

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Abstract

This paper described the research progress of biocontrol microorganisms (bacteria, fungi) and their main mechanisms for the biocontrol of microbial plant pathogens. Meanwhile, this paper also briefly described the symptoms, pathogenesis, and influencing factors of tobacco black shank disease. Based on the current progress of biocontrol microorganisms to prevent and control tobacco black shank disease, this paper suggested future research directions such as broadening the screening scope of biocontrol microorganisms, delving into the molecular mechanisms of biocontrol microorganisms, utilizing genetic engineering technology to enhance biocontrol microorganisms. Moreover, the possibility of combining biocontrol microorganisms with new materials such as nanomaterials was discussed in order to achieve better prevention and control effects.

Key words

tobacco black shank / Phytophthora nicotianae / biological control / biocontrol bacteria / biocontrol fungus

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HAO Haohao , ZHANG Yuwei , TANG Peipei , et al . Research Progress on Biocontrol Microorganisms Against Phytophthora nicotianae[J]. Journal of Agriculture. 2025, 15(5): 41-47 https://doi.org/10.11923/j.issn.2095-4050.cjas2024-0041

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Cited in this article [1] Abstract

为探讨烟草根际生防细菌的生防机制，从重庆地区连作烟田健康烟株根际土壤中分离筛选到1株对烟草疫霉具有较强拮抗作用和对黑胫病具有良好防效的细菌菌株P-72-10。根据培养性状、形态特征、生理生化特性、基因组DNA的(G+C)mol%含量测定以及16S rDNA基因序列分析确定该菌株的分类地位。该菌株菌落乳白色，能产生水溶性荧光色素，革兰氏染色反应阴性，菌体杆状、大小(8.1~16.2)μm×(1.8~4.8)μm，单端生鞭毛，不形成芽孢。The BIOLOG GN2 结果显示该菌株属于假单胞菌属Pseudomonas。该菌株基因组DNA的(G+C)mol%含量为 60.72 mol%。16S rDNA基因序列分析显示该菌株与假单胞菌属荧光假单胞杆菌多个菌株的序列同源性达到99%，GenBank上的登录号为：HQ888871。结合其形态特征和生理生化特性，将菌株P-72-10鉴定为荧光假单胞杆菌P. fluorescens。平板检测拮抗代谢产物结果表明：该菌株具有分解纤维素、蛋白质和结合Fe <sup>3+</sup>的能力，但不能分解几丁质。

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https://doi.org/10.16409/j.cnki.2095-039x.2019.06.022

Cited in this article [1] Abstract

为获得对烟草黑胫病和根黑腐病具有双重防病效果且能够促进烟草生长的假单胞杆菌，采用稀释涂布法从40份土壤样品中分离出201株细菌，通过平板对峙和含毒介质法，筛选出对烟草黑胫病和烟草根黑腐病病原菌均具有良好拮抗作用的菌株PA2101和PG3402。盆栽促生试验表明，菌株PA2101和PG3402能协调地改善烟草地上部分的生长和烟草根系发育，均在一定程度上增加了烟草的株高、叶面积、株鲜重、根鲜重、叶片数和根长，提高了烟草的根冠比和根活力。盆栽试验结果表明，菌株PA2101对烟草黑胫病和根黑腐病的防效分别为70.11%和62.67%，均高于其对照药剂；菌株PG3402对两种病害的防效分别为60.92%和60.00%，与对照药剂相当。抗性标记菌株的定殖试验结果表明，菌株PA2101和PG3402在接种后第29 d能定殖于烟草根际土壤和根内，在烟草茎和叶内也能长时间存在，表明两菌株能够良好定殖。16S rDNA序列、菌落形态和生理生化性状分析表明，菌株PA2101为铜绿假单胞杆菌Pseudomonasaeruginosa，菌株PG3402为格拉纳达假单胞杆菌Pseudomonas granadensis。综上所述，菌株PA2101和PG3402对烟草具有良好的促生作用，并对烟草黑胫病和根黑腐病有较好的防病效果，是具有生防潜力的菌株。

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