Effects of Low Temperature Stress on Physiology and Biochemistry in Seed Germination of Castor Bean

doi:10.11923/j.issn.2095-4050.cjas16100008

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Journal of Agriculture ›› 2017, Vol. 7 ›› Issue (3) : 5-8. DOI: 10.11923/j.issn.2095-4050.cjas16100008

Effects of Low Temperature Stress on Physiology and Biochemistry in Seed Germination of Castor Bean

张春兰

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Abstract

The purpose of the study is to explore the regulation mechanism of castor seed germination under low temperature, in order to select and breed new varieties with high quality and resistance. In this study, three castor cultivars were used as materials. A complete randomized design was adopted with 3 duplicates. The low temperature of 15℃ and normal temperature of 25℃ (CK) were set up and the seeds germination experiments were conducted. After determining the vitality of cultivars, the physiological index was determined by taking samples when the radicles were 2 cm long. The results indicated that seeds germination of castor was significantly hindered by low temperature stress. The sugar content and TSP were less than that of the control in radicles under low temperature, but the MDA content was significantly higher than that of the control under the low temperature. The performance of enzyme activity in castor was quite different under low temperature, SOD activity in radicles was less than that of the control under low temperature. Excepting‘Tongbi No.6’, other varieties’POD activity was significantly lower than that of the control; the CAT activity performance was not significant, and the activities were basically coincident under different temperature conditions. It concluded that‘Tongbi No.5’performed well under low temperature stress, and it could take gene sequencing for the next step.

Key words

Castor; Low Temperature Stress; Seed Germination; Physiology and Biochemistry

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张春兰. Effects of Low Temperature Stress on Physiology and Biochemistry in Seed Germination of Castor Bean[J]. Journal of Agriculture. 2017, 7(3): 5-8 https://doi.org/10.11923/j.issn.2095-4050.cjas16100008

References

[1].Koornneef M,Bentsink L,Hilhorst H.Seed dormancy and germination[J].Current Opinion in Plant Biology,2002,5(1) :33-36.
[2].Henderson I R,Dean C.Control of arabidopsis flowering: the chill before the bloom[J].Development,2004,131(16) :3829- 3838.
[3].Gray W M,Qstin A,Sandberg G,Romano C P,Estelle M.High temperature promotes auxin-mediated hypocotyl elongation in Arabidopsis[J].Proceedings of the National Academy of Sciences,1998,95(12): 7197-7202.
[4].Mazzella M A,Bertero D,Casal J J. Temperature-dependentinternode elongation in vegetative plants of Arabidopsis thaliana lacking phytochrome B and cryptochrome 1[J]. Planta,2000,210(3): 497-501.
[5].Penfield S.Temperature perception and signal transduction in plants[J].New Phytologist,2008,179(3) :615-628.
[6].魏乐,杜军华.高温和零上低温对小麦根系含糖量及细胞透性的影响[J].青海师范大学学报:自然科学版,1999,(1):36- 39.
[7].Baldwin BS, Cossar RD. Castor yield in response to planting date at four locations in the south-central United States. Industrial Crops and Products, 2009, 29:316-319.
[8].Ogunniyi DS. Castor oil: a vital industrial rawmaterial. Bioresource Technol, 2006, 97:1086–1091.
[9]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000,(7):184,164-167.
[10].马艳青, 戴雄泽. 2000.低温胁迫对辣椒抗寒性相关生理指标的影响[J].园艺学进展(第四辑),哈尔滨工程大学出版社, 374 ￥
[11].Gao Y,Qi X H,Yang J H,Zheng M F.The response mechanism of cold stress in higher plants[J].Northern Horticulture,2007(10) :58-61．
[12].Lu M H,Chen J F.2004.Genetic engineering of plant for cold tolerance[J]. Acta Botanica Boreali-Occidentalia Sinica.24(10):1953-1958.
[13].刘大永,王维香.过氧化氢对水稻幼苗中CAT和POD活性的影响[J].作物学报:1998,24(3):320-324.
[14].Susan W,Atul M,Vaidaya G B,et al.High specific activity of whole cells in an aqueousorganic two phasemem brane bioreactor [J].Enzyme and Technology,1998(22):575-577.
[15].潘晓云.膜脂过氧化作为扁桃品种抗寒性鉴定指标研究[J],生态学报,2002(11):1902-1911.
[16].简令成,吴素萱.植物抗寒性的细胞学研究-小麦越冬过程中细胞内物质的变化[J].植物报.1965,13: 1-16.
[17].Levitt J. Responses of Plant to Environmental Stress [M]. New York: Academic Press, 1980, 365-488.
[18].王爱国,邵从本,罗广华,等.大豆下胚轴线粒体的衰老与膜脂的过氧化作用[J].植物生理学报,1988,14(3):269.
[19].李明玉,曹辰兴,于喜艳.低温锻炼对冷胁迫下黄瓜幼苗保护性酶的影响[J].西北农业学报,2006,15(1):160-164.
[20].张自坤,刘世琦,王忠全,等.低温胁迫对不同砧木黄瓜嫁接苗生理生化指标的影响[J]..山东农业科学,2009,5:36-40.
[21].徐心诚.低温弱光对黄瓜幼苗过氧化物酶同工酶和丙二醛含量的影响[J].河南农业科学,2012,14(1):113-116.
[22].王燕,李雪平,季作梁.芒果冷害对两种自由基清除剂的影响[J].园艺学报,1995,22(3):235-239.
[23].朱其杰,高守云,蔡洙湖.等.黄瓜耐冷性鉴定及遗传规律的研究[M].见:李树德主编:中国主要菜抗病育种进展[M].北京:科学出版社,1995.457-462.