Study of Different Micronutrients on Yield and Physiological Indicators of Brassica napus

ZHAOPeidong, ZHANGWei, LIUYingxia, ZHANGZhenqian

Journal of Agriculture ›› 2024, Vol. 14 ›› Issue (4) : 21-25.

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Abbreviation (ISO4): Journal of Agriculture      Editor in chief: Shiyan QIAO

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Journal of Agriculture ›› 2024, Vol. 14 ›› Issue (4) : 21-25. DOI: 10.11923/j.issn.2095-4050.cjas2023-0108

Study of Different Micronutrients on Yield and Physiological Indicators of Brassica napus

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Abstract

Rapeseed is the largest self-produced plant oil source in China, which is crucial for ensuring the safety of edible oil in China. However, the self-sufficiency rate of total rapeseed production is severely insufficient, so high yield and high efficiency cultivation measures are urgently needed to increase yield. Current research was mostly focused on traditional fertilizers such as nitrogen, phosphorus and potassium, with less research on micro fertilizers. This study used Brassica napus ‘Fanming 1’ as the experimental material to measure the physiological and biochemical indicators of the entire growth period and the yield traits under different micro-fertilizer conditions, and conducted correlation analysis between them. The results showed that the activities of peroxidase and catalase in leaves at 5-6 leaf stage and superoxide dismutase in flowers at flowering stage were more than 0.8, indicating that there had a high correlation, which could be used to predict the rapeseed yield. The fertilization conditions may increase the rapeseed yield when the POD and CAT activities in the leaves during the 5-6 leaf stage and the SOD activity in the flowers during the flowering stage were greater than 0.8, and the chlorophyll content in the flowers was greater than 0.9. The results of this study provided a reference for screening suitable micronutrient fertilizers and high-yield cultivation for rapeseed growth.

Key words

rapeseed / micronutrient fertilizer / peroxidase / superoxide dismutase / catalase

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ZHAO Peidong , ZHANG Wei , LIU Yingxia , et al. Study of Different Micronutrients on Yield and Physiological Indicators of Brassica napus[J]. Journal of Agriculture. 2024, 14(4): 21-25 https://doi.org/10.11923/j.issn.2095-4050.cjas2023-0108

References

[1]
杨文钰, 屠乃美. 作物栽培各论南方本(第三版)[M]. 北京: 中国农业出版社, 2021:1-2.
[2]
常涛, 程潜, 张振乾, 等. 高含油量油菜新品种选育研究进展[J]. 分子植物育种, 2019, 17(13):4424-4430.
[3]
王凤文. 微肥对小油菜微量元素吸收特性的影响[J]. 内蒙古农业科技, 2010(4):67-68,95.
[4]
BANERJEE A, DATTA J K, MONDAL N K. Impact of different combined doses of fertilizers with plant growth regulators on growth, yield attributes and yield of mustard (Brassica campestris, cv. B 9) under old alluvial soil of Burdwan, West Bengal, India[J]. Frontiers of agriculture in China, 2010, 4(3):341-351.
[5]
LIU X W, ZHAO Z Q, DUAN B H, et al. Effect of sulphate on selenium uptake and translocation in rape (Brassica napus L.) supplied with selenate or selenite[J]. Plant and soil, 2016, 399(1-2):295-304.
[6]
IMRAN, KHAN A A. Canola yield and quality enhanced with sulphur fertilization[J]. Russian agricultural sciences, 2017, 43(2):113-119.
[7]
黄窈军, 谢炜, 倪中应, 等. 水溶硼肥喷施在油菜浙油50上的应用效果[J]. 浙江农业科学, 2021, 62(11):2148-2149,2151.
[8]
张衡锋, 张焕朝, 韦庆翠, 等. 4种微肥对番红花生长、品质及产量的影响[J]. 南京林业大学学报(自然科学版), 2016, 40(1):169-173.
为探明大田条件下铁、锌、锰、铜4种微量元素对番红花生长、品质及产量的影响,采用叶面喷施的方法,比较不同种类和浓度的微肥处理对番红花叶片光合特性、仔球中碳水化合物的积累及种球产量的影响。结果表明:叶面喷施适量微肥可显著提高叶片净光合速率,尤以Zn肥影响最显著,增强了叶片的“代谢源”能力; 适量喷施4种微肥均可极显著增加仔球的可溶性糖、可溶性蛋白和淀粉含量,也增强“代谢库”的功能,进而显著增加仔球产量和总数。施肥量以喷施0.20%铁、0.10%锌、0.12%锰和0.10%铜的处理效果为最佳; 但过量施用会产生负效应,抑制番红花的生长,并降低其品质和产量。
[9]
MARSCGNER H. General introduction to the mineral nutrition of plants[M]. Inorganic Plant Nutrition, 1983.
[10]
RUBIO G, MARTIN O, CARINA RA, et al. Mechanisms for the increase in phosphorus uptake of waterlogged plants: Soil phosphorus availability, root morphology and uptake kinetics[J]. Oecologia, 1997, 112(2):150-155.
Waterlogging frequently reduces plant biomass allocation to roots. This response may result in a variety of alterations in mineral nutrition, which range from a proportional lowering of whole-plant nutrient concentration as a result of unchanged uptake per unit of root biomass, to a maintenance of nutrient concentration by means of an increase in uptake per unit of root biomass. The first objective of this paper was to test these two alternative hypothetical responses. In a pot experiment, we evaluated how plant P concentration of Paspalum dilatatum, (a waterlogging-tolerant grass from the Flooding Pampa, Argentina) was affected by waterlogging and P supply and how this related to changes in root-shoot ratio. Under both soil P levels waterlogging reduced root-shoot ratios, but did not reduce P concentration. Thus, uptake of P per unit of root biomass increased under waterlogging. Our second objective was to test three non-exclusive hypotheses about potential mechanisms for this increase in P uptake. We hypothesized that the greater P uptake per unit of root biomass was a consequence of: (1) an increase in soil P availability induced by waterlogging; (2) a change in root morphology, and/or (3) an increase in the intrinsic uptake capacity of each unit of root biomass. To test these hypotheses we evaluated (1) changes in P availability induced by waterlogging; (2) specific root length of waterlogged and control plants, and (3) P uptake kinetics in excised roots from waterlogged and control plants. The results supported the three hypotheses. Soil P avail-ability was higher during waterlogging periods, roots of waterlogged plants showed a morphology more favorable to nutrient uptake (finer roots) and these roots showed a higher physiological capacity to absorb P. The results suggest that both soil and plant mechanisms contributed to compensate, in terms of P nutrition, for the reduction in allocation to root growth. The rapid transformation of the P uptake system is likely an advantage for plants inhabiting frequently flooded environments with low P fertility, like the Flooding Pampa. This advantage would be one of the reasons for the increased relative abundance of P. dilatatum in the community after waterlogging periods.
[11]
常涛, 张振乾, 陈浩, 等. 不同含油量生理生化指标和种皮结构分析[J]. 分子植物育种, 2019, 17(23):7871-7878.
[12]
常涛, 谢鑫, 官梅, 等. 不同微肥处理下油菜产量与高光谱反射率关系分析[J]. 光谱学与光谱分析, 2021, 41(2):552-557.
[13]
萧浪涛, 王三根. 植物生理学实验技术[M]. 北京: 中国农业出版社, 2014:110-114.
[14]
王寅, 鲁剑巍. 中国冬油菜栽培方式变迁与相应的养分管理策略[J]. 中国农业科学, 2015, 48(15):2952-2966.
直播和育苗移栽是目前长江流域冬油菜并存的两种栽培方式,其技术发展和推广状况对中国油菜产业发展和油料供应安全具有重要意义。不同的历史阶段下,随栽培品种、种植目标、劳动力条件和生产力水平等因素的变化,中国冬油菜栽培方式也在不断的转变和发展。文章概述了新中国建立后冬油菜栽培方式的发展变化历程,从最初以直播种植为主,到20世纪60年代中期直播为主而育苗移栽开始起步,20世纪80年代育苗移栽方式实现全面推广并长期应用,再到当前直播和育苗移栽两种栽培方式并存。而养分管理措施也经历了从最初施用农家肥,到开始施用氮、磷化肥,到提倡氮、磷、钾、硼肥配合施用,再到当前形成的育苗移栽油菜高产高效养分管理技术。直播和育苗移栽油菜的栽培特点和生长过程存在着显著差异,文章主要从生育进程、种植密度、群体结构与个体形态等方面对两者进行了分析和比较。相比育苗移栽油菜,直播油菜的生育期一般相应缩短,个体生长状况较弱,单株产量偏低,但在较高的种植密度基础上可发挥群体优势,且根群结构也有助于增强养分和水分吸收能力,因此具有高产高效的潜力。目前,长江流域育苗移栽油菜的养分丰缺指标和推荐施肥体系已经建立,养分管理策略也较为完善,其要点为:根据土壤肥力状况和目标产量水平合理确定氮、磷、钾、硼肥用量,保证养分平衡供应,实行有机无机肥料配合施用,采取氮、钾肥分次施用(推荐基肥﹕越冬肥﹕薹肥=60%﹕20%﹕20%)以协调生长发育和养分吸收,强壮个体而实现高产。针对直播油菜发展迅速而在高产条件下养分管理研究相对滞后的现状,文章重点比较了其与育苗移栽油菜在养分响应、吸收分配和需求利用等方面的差异,发现直播油菜对养分缺乏较为敏感,养分不足导致个体生长低下和群体衰亡,后期物质和养分转运效率较低,而且对磷、钾养分的需求明显更高。基于已有研究结果总结出直播油菜的“前促后稳”养分管理策略,即在氮、磷、钾及微量元素养分合理平衡施用基础上,保证磷、钾供应,施用有机肥并进行秸秆还田,肥料运筹中重视氮肥对个体和群体发展的调控作用,减少基施而增加苗肥以促前期生长,中后期适时追肥以保证群体数量和产量形成,推荐基肥﹕苗肥﹕越冬肥﹕薹肥=40%﹕30%﹕15%﹕15%。此外,应配合其他栽培措施协同增效提质,选用适宜早熟和耐密的品种,采用机械化精量播种技术,施用长效专用复合肥或专用控释肥,配合密植以省肥补迟、增库促源,加强病虫草害防控技术。文章还对直播油菜养分管理研究中的问题和未来方向进行了探讨和展望,以期为进一步完善其栽培管理措施和科学施肥技术提供参考。
[15]
余涛. 植物逆境相关基因的克隆、表达及功能分析[D]. 武汉: 武汉大学, 2004.
[16]
郑瑞, 师尚礼, 马史琛. 苜蓿、小麦自毒及他感作用机理[J]. 草业科学, 2019, 36(3):849-860.
[17]
白鹏, 冉春艳, 谢小玉. 干旱胁迫对油菜蕾薹期生理特性及农艺性状的影响[J]. 中国农业科学, 2014, 47(18):3566-3576.
【目的】针对长江流域特别是长江上游近几年春季降水偏少易导致干旱的特点,研究水分胁迫对油菜蕾薹期生理特性及农艺性状的影响,为揭示油菜蕾薹期抗旱机制及抗旱品种选育提供理论依据。【方法】采用盆栽的方式,在遮雨网室对两种抗旱性不同的油菜品种中双10号(抗旱性弱)和94005(抗旱性强)蕾薹期进行干旱胁迫,以各性状的抗旱系数研究油菜相关生理指标及农艺性状的变化,在此基础上筛选出适用于该区域油菜蕾薹期抗旱性鉴定的指标。【结果】随着干旱时间的延长,两种油菜的叶片净光合速率、气孔导度、蒸腾速率、胞间二氧化碳浓度、RWC、叶绿素含量、RuBP羧化酶活性、株高、茎粗、一次分枝数以及单株产量抗旱系数均呈下降趋势,下降幅度与胁迫时间成正相关。其中,气孔导度、蒸腾速率抗旱系数下降幅度与材料抗旱性成正相关,其余指标则与材料抗旱性成负相关。抗旱性弱的材料气孔限制值、水分利用率抗旱系数分别呈现出上升-下降与一直下降的趋势,抗旱性强的材料则均为上升趋势。POD、SOD、CAT活性、可溶性糖以及可溶性蛋白相对值随着干旱胁迫时间的延长先升高后下降,抗旱性强的材料增加幅度高于抗旱性弱的材料;细胞膜透性、丙二醛(MDA)含量和脯氨酸相对值随干旱胁迫时间的延长呈上升趋势。对干旱胁迫下的油菜蕾薹期和复水后的成熟期相关指标之间的关系进行分析表明,干旱胁迫下,产量与净光合速率、RWC、叶绿素含量和一次分枝数抗旱系数呈极显著正相关,与细胞膜透性、MDA含量抗旱系数呈显著负相关。主成分分析将单株产量、净光合速率、胞间二氧化碳浓度、气孔限制值、水分利用率、RWC、叶绿素、RuBP羧化酶、SOD、POD、CAT、细胞膜透性、MDA、可溶性糖、株高、茎粗及一次分枝数划分为第一主成分;将叶片气孔导度、蒸腾速率、脯氨酸、可溶性蛋白划分为第二主成分。【结论】第一主成分的各指标(单株产量、净光合速率、胞间二氧化碳浓度、气孔限制值、水分利用率、RWC、叶绿素、RuBP羧化酶、SOD、POD、CAT、细胞膜透性、MDA、可溶性糖、株高、茎粗及一次分枝数)变化与品种抗旱性密切相关,在油菜抗旱品种选育时可作为油菜蕾薹期抗旱性鉴定的主要指标,第二主成分的各指标(叶片气孔导度、蒸腾速率、脯氨酸、可溶性蛋白)则是次要鉴选指标。
[18]
姚磊. 大豆抗氧化多糖制备表征及对辐射诱导氧化应激抑制作用[D]. 哈尔滨: 哈尔滨工业大学, 2014.
[19]
张晓红, 冯梁杰, 杨特武, 等. 冬季低温胁迫对油菜抗寒生理特性的影响[J]. 植物生理学报, 2015, 51(5):737-746.
[20]
刘海卿, 孙万仓, 刘自刚, 等. 北方寒旱区白菜型冬油菜抗寒性与抗旱性评价及其关系[J]. 中国农业科学, 2015, 48(18):3743-3756.
【目的】研究冬油菜的抗寒性和抗旱性,探讨抗寒与抗旱之间的关系,为中国北方白菜型冬油菜的改良及抗寒性和抗旱性的综合评价提供可借鉴的方法和理论依据。【方法】分别通过自然降温处理(15℃—-5℃)和人工控制水分的方法(干旱胁迫4、7和10 d)分别对6份不同抗寒等级冬油菜摸拟低温和干旱胁迫,分析其形态、生理生化和生长指标的变化,采用隶属函数法、相关性分析法、聚类分析法、主成分分析法对不同品种的抗寒性和抗旱性进行综合评价。【结果】6份冬油菜品种越冬率相差很大(20.1%—94.7%)。抗寒性强的品种植物学形态特征表现为幼苗匍匐贴地生长、生长点洼陷低于地表、叶色深绿色、真叶刺毛多。且低温胁迫之后抗寒生理生化指标变化明显,相对电导率和MDA(丙二醛)含量增加,且抗寒性强的品种增加幅度小;SOD、POD、CAT酶活性升高,可溶性蛋白、可溶性糖、游离脯氨酸等调节性物质含量明显增加,且抗寒性强的品种变化明显,差异显著。随着干旱胁迫时间延长,膜结构首先遭到破坏,相对电导率和MDA含量升高,细胞失水,叶片相对含水量、束缚水/自由水、叶绿素含量降低(光合作用降低),幼苗苗长、叶片和根鲜干重降低,直到幼苗萎蔫,且抗旱性强的品种变化幅度小,同时抗旱性强的品种叶片保水能力强、土壤耗水少、萎蔫系数小。通过主成分分析,6份冬油菜的抗寒性强弱依次为陇油7号>陇油6号>陇油9号>延油2号>天油2号>Vision,而抗旱性强弱依次为陇油6号>陇油7号>陇油9号>延油2号>Vision>天油2号。【结论】中国北方寒旱区低温、干旱并存,不同冬油菜品种间抗寒性和抗旱性差异较大,由于产生了交叉适应性,在抵御低温冻害的同时提高了对干旱胁迫的适应性,因此,白菜型冬油菜抗寒性强的品种一般抗旱性也比较强。
[21]
杨顺强. 不同禾本科牧草在干旱胁迫和低温胁迫下的生理响应[D]. 杨陵: 西北农林科技大学, 2008.
[22]
刘林波, 孙万仓, 刘自刚, 等. 白菜型冬油菜抗寒相关生理生化性状的配合力和遗传效应[J]. 中国油料作物学报, 2015, 37(6):772-779.
[23]
陆志峰, 任涛, 鲁剑巍, 等. 缺钾油菜叶片光合速率下降的主导因子及其机理[J]. 植物营养与肥料学报, 2016, 22(1):122-131.
[24]
马霓, 肖圣元, 张明海, 等. 栽培方式对油菜'中双11'生长及收获特性的影响[J]. 湖北农业科学, 2011, 50(2):244-247.
[25]
刘婷婷, 孙盈盈, 曹石, 等. 油菜抗裂角性状研究进展[J]. 作物杂志, 2014(3):5-9.
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