The self-healing oxidized sodium alginate-glycol chitosan hydrogel （OSA-GC） based on dynamic imine bonds was synthesized. Oxidized sodium alginate （OSA） was synthesized by oxidizing sodium alginate with sodium periodate， and self-healing OSA-GC hydrogels with different cross-linking degrees were prepared by Schiff base reaction with glycol chitosan （GC）. The effect of GC concentration on the microscopic morphology，viscoelasticity，swelling performance，self-repair performance， degradation rate and in vitro drug release performance of OSA-GC hydrogels were investigated. The results show that OSA-GC hydrogels have the characteristics of porous structure with a pore size ranging from 50 μm to 280 μm by controlling the mass ratio of OSA to GC. The OSA-GC hydrogels can reach the swelling equilibrium at 120 hours， and the swelling ratio reach 71.3-112.1. OSA-GC hydrogel can be degraded in PBS containing lysozyme （10 mg/mL）， and the mass loss of OSA-GC hydrogel is 43.1%-51.9% after 12 days. At room temperature， OSA-GC hydrogels can achieve self-healing within 2 h in the absence of external stimuli. OSA-GC hydrogel loaded with gemcitabine shows a sustained release effect on the anti-cancer drug gemcitabine， and the release time can reach 48 hours in the drug release experiment， which shows promising application prospects in biomedical fields of drug carriers.