As a type of biomimetic catalyst， artificial enzymes can effectively overcome the limitations of natural enzymes in purification， storage， and recyclability. Hemin （Fe（Ⅲ）-protoporphyrin Ⅸ）， serving as the essential cofactor in the active center of most peroxidases， possesses fundamental peroxidase-like catalytic activity due to its iron-porphyrin structure. However， native free hemin suffers from issues such as intermolecular self-aggregation， susceptibility to oxidative deactivation， and insufficient exposure of catalytic sites， leading to reduced catalytic efficiency and poor stability. Combining hemin with supporting materials to form hemin-based artificial enzymes can effectively inhibit hemin self-aggregation and oxidative degradation while simultaneously enhancing its catalytic activity and stability. This review primarily introduces several common types of hemin-based artificial enzymes. It summarizes and categorizes their construction and applications based on the underlying principles of the various support materials and the characteristics of the resulting hemin-based enzymes. Furthermore， it analyzes how the structural properties of different supports regulate the functions of the artificial enzymes and provides an outlook on their future development. Current challenges in designing and constructing hemin-based artificial enzymes include complex self-assembly processes and poor controllability during preparation. Future studies could focus on conducting in-depth physicochemical research on support materials to achieve a higher integration of hemin and support properties. This may involve establishing structure-activity relationship maps correlating the physicochemical properties of supports with the directional assembly of hemin molecules， implementing interface engineering strategies for synergistic optimization of hemin and carrier performance， or exploring alternative support materials with similar properties. The development of hemin-based artificial enzymes combining high catalytic activity with structural homogeneity is key to facilitating their practical applications across multiple fields.

Contents

1 Introduction

2 Hemin

3 Synthesis and application of hemin-based artificial enzymes

3.1 Carbon-supported hemin artificial enzymes

3.2 MOF-supported hemin artificial enzymes

3.3 Integration and applications of hemin-functionalized inorganic supports

3.4 Research and application of synthetic polymer-immobilized Hemin​

3.5 Conjugation and applications of Hemin with biomacromolecules​

4 Conclusion and outlook