In order to screen for Huai'an Medicinal Celery (HY) strains that are more suitable for cultivation in the Huai'an region and have excellent comprehensive quality, ‘HY-1’, ‘HY-2’, ‘HY-3’ and ‘HY-4’ were used as experimental materials to compare agronomic traits, photosynthetic characteristics and nutritional quality using significant difference analysis and correlation analysis. The results showed that ‘HY-3’ was dominant in the four lines except for leaflet width, plant height and spread degree. The most obvious advantages were the number of leaflet (172.67 per plant) and plant weight (256.60 g/per plant). Among the four lines, ‘HY-3’ had the highest net photosynthetic rate and water use efficiency, while ‘HY-4’ had the highest transpiration rate, stomatal conductance and intercellular CO₂ concentration. Leaflet chlorophyll content (42.13 SPAD), apigenin (1.55 mg/g in leaflet, 0.88 mg/g in petiole) and luteolin (1.77 mg/g in leaflet, 0.28 mg/g in petiole) of ‘HY-3’ were the highest among the 4 lines, and lignin content was relatively high (12.82 mg/g in leaflet, 10.17 mg/g in petiole). The correlation analysis showed that the leaflet length and leaflet width were positively correlated, and the contents of apigenin and luteolin were positively correlated in the 4 ‘HY’ lines. In summary, the ‘HY-3’ has better agronomic traits, photosynthetic capacity, water utilization rate and nutritional quality, and has higher adaptability to stress and high yield potential.