To clarify the comparative advantage characteristics of corn planting regions in Yunnan Province, based on the corn planting region and yield data of 16 prefectures and cities in Yunnan Province from 2000 to 2020, production scale index, production concentration index, scale comparative advantage index, efficiency comparative advantage index, and comprehensive comparative advantage index were used to calculate and analyze. The results showed that: (1) the corn planting regions in Yunnan Province were concentrated in the northeast and south, especially in Qujing, Zhaotong, and Wenshan. (2) Qujing, Zhaotong, Wenshan, Pu'er, and Lincang had contributed the most to the total corn planting area in the province, which concentrated more than half of the total maize planting area in the province. (3) Qujing, Zhaotong, Wenshan, Dali, and Honghe had contributed the most to the province's corn yield, accounting for more than half of the total corn yield in the province. (4) The comparative advantage of corn production scale in Yunnan Province was generally on the rise, while the comparative advantage of corn production efficiency and comprehensive comparative advantage were generally stable. The comprehensive advantageous production regions had been formed in the northeast regions such as Zhaotong and Qujing, the southeast regions such as Wenshan, and the southwest regions such as Lincang, and the northwest regions such as Nujiang and Diqing. The scale comparative advantage accelerated the continuous enhancement of the polarization effect of comprehensive comparative advantage. In order to accelerate the high-quality development of the corn industry in Yunnan Province, it is necessary to continuously ensure policy support for advantageous corn planting regions, gradually tap into the production potential of corn planting comparative advantages, and appropriately reduce the proportion of corn in non advantageous corn planting regions.