Brown spot of pear, caused by Stemphylium vesicarium, is a fungal disease of increasing importance in several pear-growing areas of Europe. Disease control measures include the application of fungicides and sanitation methods. Antimicrobial peptides may be a complement or alternative to conventional fungicides used to manage brown spot disease. In a previous study, the synthetic peptide BP15 showed postinfection fungicidal activity against S. vesicarium in in vitro and detached-leaf assays. In the present study, the efficacy of BP15 (KKLFKKILKVL-NH) in controlling brown spot of pear was evaluated under field conditions using potted plants and pear trees in orchards. In field trials, the treatments with BP15 or with the fungicide thiram were scheduled according to the infection risk predicted by the BSPcast model. Potted pear plants treated with BP15 showed a disease reduction of about 42 to 60% in five of seven trials. In three of four tree trials, the disease severity on shoots treated with BP15 was significantly lower than in the nontreated controls, with a mean efficacy of 38.2%. It was concluded that BP15 is a good candidate to be further developed as a fungicide for controlling brown spot of pear.

为了筛选各品种棉花根际土壤中的拮抗真菌,探索其拮抗机制,以及测定其对棉花种子的安全性。本试验利用稀释平板法,从6个陆地棉品种（中棉所08、中棉所41、中植棉2、冀棉11、鲁棉研28和鲁棉研21）的根际土壤中分离出真菌;用拮抗培养的方法筛选出对赤霉病菌（Fusarium graminearum）F0609和大丽轮枝菌（Verticillium dahliae ）V1070有拮抗作用的真菌;用扫描电镜观察其抑制作用;用浸染法进行种子发芽试验。结果表明,从棉花根际土壤中分离出64株对赤霉病菌F0609具有抑制作用的真菌;筛选10株进行18S rDNA测序,挑选4株与大丽轮枝菌V1070对峙培养,其中黑曲霉（Aspergillus niger）An1对V1070的平均抑菌率达91%,棘孢木霉Tr1（Trichoderma asperellum）抑菌率为87%,高山被孢霉Mo1（Mortierella alpina）抑菌率为76%,层出镰孢霉Fu1（Fusarium laminum sp.）抑制率为70%;拮抗真菌黑曲霉An1能使大丽轮枝菌V1070菌丝破裂,产孢增多;黑曲霉An1对棉花种子发芽率和鲜重积累无明显影响。经筛选鉴定了10株赤霉病菌F0609的拮抗真菌,其中4株对大丽轮枝菌V1070拮抗作用较好,其中An1能使大丽轮枝菌菌丝破裂,且对棉花种子安全性无影响。

Plant diseases caused by pathogenic fungi are devastating. However, commonly used fungicides are harmful to the environment, and some are becoming ineffective due to fungal resistance. Therefore, eco-friendly biological methods to control pathogenic fungi are urgently needed.

The continuous cropping barrier is an important factor leading to the decline of watermelon quality and yield. In this study, we focused on a bio-organic fertilizer prepared with one bacterial strain, Bacillus sp. XG-1, to prevent the occurrence of the continuous cropping barrier. The strain XG-1 was isolated from watermelon rhizosphere soil, and promoted the growth of watermelon by producing phytase (0.19 U/mL), indole-3-acetic acid (IAA, 7.31 mg/L), and gibberellins (GA3, 2.47 mg/L). In addition, the strain also possessed a strong antagonistic effect against the pathogen Fusarium oxysporum f. sp. niveum (Fon) by inhibiting conidia germination with an inhibition ratio of 85.3% and mycelium growth. The bio-organic fertilizer fermented by XG-1, based on cow manure compost and rapeseed meal (85:15, w/w) under optimal conditions, was mixed in soil (watermelon had been planted for two consecutive years). After the cultivation of watermelon for 50 d, a higher density of XG-1 (9.79 × 105 colony-forming units (CFU)/g) and one order of magnitude lower of Fon (1.29 × 103 copies/g) were detected in the rhizosphere soil compared with soils without bio-organic fertilizer (7.59 × 104 copies/g for Fon), leading to an 86.4% control efficiency of watermelon caused by Fusarium wilt. The application of bio-organic fertilizer enriched soil nutrients, including the organic matter (13.2%), total nitrogen (13.9%), total phosphorus (20.5%), and total potassium (3.77%), adjusted the soil pH from 6.69 to 7.01, and significantly improved the watermelon growth in terms of the seedling height, root length, fresh weight of seedling and root with increase of 78.8%, 72.2%, 84.6%, and 96.4%, respectively. This study regarded the watermelon continuous cropping soil as the research point, and focused on inhibiting Fon, regulating soil properties and enhancing watermelon growth to eliminate the continuous cropping barrier through a combination of compost and functional strains, demonstrating the potential application value in watermelon production.

The accumulation of atrazine in farmland is prone to cause phytotoxicity to kinds of sensitive crops, such as soybean. In addition, some kinds of agricultural solid wastes have long been considered as the important non-point pollution source. The aim of this experiment was to investigate the feasibility of removing atrazine from soil and alleviating the stress of atrazine on the growth of soybean by application a novel bio-organic fertilizer developed by agricultural solid wastes, such as cow manure organic fertilizer, biochar and poly-(γ-glutamic acid), as well as an atrazine-degrading strain Arthrobacter sp. DNS10. Sixteen potential bio-organic fertilizer formulations were designed by D-optimal mixture design of Design Expert software and atrazine-removal ability was selected to single out the optimal formulation. As a result, the optimal formulation of bio-organic fertilizer (named as DNBF10) was produced by the cow manure organic fertilizer 76.20%, biochar 4.46%, poly-(γ-glutamic acid) 8.63% (m/m) and the number of Arthrobacter sp. DNS10 with 0.91 × 10 CFU/g. The atrazine removal percentage of DNBF10 for the atrazine in soil with the initial atrazine concentration 15.26 ± 0.49 mg/kg was 95.05% after 10 days' application with DNBF10 at the adding dosage of 5 mg/kg (relative to the dry weight of the soil). Furthermore, pot experiment results suggest that the growth of soybean seedlings in the soil (initial atrazine was 8.14 ± 0.16 mg/kg) that adding both of DNBF10 (25%) and chemical fertilizer (75%) were better than those of the treatment only adding chemical fertilizer (100%) under the same nutrient addition level. All the results indicate that the application of DNBF10 was a new alternative to reuse the typical agricultural solid wastes, as well as to reduce the harm caused by residual atrazine to soybean.Copyright © 2018 Elsevier Ltd. All rights reserved.

Pectate lyases play an important role in pectin degradation, and therefore are highly useful in the food and textile industries. Here, we report on the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1. The full-length gene (1350 bp) encodes for a 449-residue protein that belongs to the polysaccharide lyase family 9 (PL9). Recombinant PpPel9a produced in Escherichia coli was purified to electrophoretic homogeneity in a single step using Ni2+-NTA affinity chromatography. The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins. Using HG-type pectins as substrate, PpPel9a showed greater activity with de-esterified HGs. In addition, PpPel9a was active against water-soluble pectins isolated from different plants. Using this lyase, we degraded citrus pectin, purified fractions using Diethylaminoethyl (DEAE)-sepharose column chromatography, and characterized the main fraction MCP-0.3. High-performance gel permeation chromatography (HPGPC) analysis showed that the molecular mass of citrus pectin (~230.2 kDa) was reduced to ~24 kDa upon degradation. Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin. In vitro testing showed that the degradation product MCP-0.3 significantly promotes the growth of Lactobacillus plantarum and L. rhamnosus. In this regard, the enzyme has potential in the preparation of pharmacologically active pectin products.

\n Polymyxins exhibit antibacterial activity against various gram-negative bacteria. However, the regulatory mechanisms of polymyxin synthesis remain unclear. Here, Abh, AbrB3, and Spo0A of\n Paenibacillus polymyxa\n SC2 were found to bind to P\n \n pmx\n \n via DNA pull-down and electromagnetic mobility shift assays. Oxford Cup and liquid chromatography-mass spectrometry assays showed that antibacterial activity and polymyxin production increased in the Δ\n abh\n strain. Overexpression of\n abh\n and\n abrB3\n significantly decreased the antibacterial activity and polymyxin production. The transcription of\n pmxA\n determined via quantitative reverse transcription-PCR supported these results. Moreover, the mutation and complementation of\n spo0A\n revealed that Spo0A was an activator of polymyxin synthesis. Spo0A could bind to the\n abh\n promoter to activate\n abh\n expression. Spo0A could bind to the\n abrB3\n promoter to inhibit\n abrB3\n transcription and mitigate the inhibitory effect of AbrB3 on\n abh\n transcription.\n

The bacterium Paenibacillus polymyxa is found naturally in diverse niches. Microbiome analyses have revealed enrichment in the genus Paenibacillus in soils under different adverse conditions, which is often accompanied by improved growth conditions for residing plants. Furthermore, Paenibacillus is a member of the core microbiome of several agriculturally important crops, making its close association with plants an interesting research topic. This review covers the versatile interaction potential of P. polymyxa with plants and its applicability in industry and agriculture. Thanks to its array of produced compounds and traits, P. polymyxa is potentially an efficient plant growth-promoting bacterium, with the potential of biofertilization, biocontrol and protection against abiotic stresses. By contrast, cases of phytotoxicity of P. polymyxa have been described as well, in which growth conditions seem to play a key role. Because of its versatile character, we propose this bacterial species as an outstanding model for future studies on host-microbe communications and on the manner how the environment can influence these interactions. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.

Paenibacterin A1 (PA1) is a broad-spectrum, cationic cyclic lipodepsipeptide antibiotic isolated from. In this study, the roles of the cationic residues and lipid tail length on the in vitro antibacterial and hemolytic activities of PA1 was examined in the context of an active PA1 analogue, called PAK, in which the two D-Orn residues in PA1 were converted to D-Lys residues. The effect of reducing the length of the lipid tail in PAK from 15 to 12-10 carbons on the minimum inhibitory concentration (MIC) depended upon the bacteria. This change had little effect on the MIC against and but resulted in a reduction in activity against most of the ESKAPE pathogens tested with the exception of. Any one of the four cationic residues in PAK could be replaced with alanine with only a minimal effect on its MIC against,,,, and MSSA. For and the two MRSA strains tested, the presence of cationic residues at positions 7 and 12 are not important for activity, while the cationic residues at positions 1 and 4 are important. While PAK exhibited some hemolysis at 8 μg/mL and 70% hemolysis at 128 μg/mL, its C-12 and C-10 analogues were not hemolytic up to 128 μg/mL. All PAK analogues that had one or two cationic residues replaced with alanine were as hemolytic as or more hemolytic than PAK.

An antibiotic produced by Paenibacillus polymyxa 7F1 was studied. The 7F1 strain was isolated from the rhizosphere of a wheat field. Response surface methodology was used to optimize the physicochemical parameters. The strain showed broad-spectrum activity against several plant pathogens. Identification of the strain was realized based on 16s rRNA gene and gyrB gene sequencing. The antibiotic was optimized by one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches. The suitable antibiotic production conditions were optimized using the one-factor-at-a-time method. The individual and interaction effects of three independent variables: culture temperature, initial pH, and culture time, were optimized by Box-Behnken design. The 16SrRNA gene sequence (1239 nucleotides) and gyrB gene (1111 nucleotides) were determined for strain 7F1 and shared the highest identities to those of Paenibacillus polymyxa. The results showed the optimal fermentation conditions for antibiotics produced by Paenibacillus polymyxa 7F1 were a culture temperature of 38 °C, initial pH of 8.0, and culture time of 8 h. The antibiotics produced by Paenibacillus polymyxa 7F1 include lipopeptides such as iturin A and surfactin. The results provide a theoretical basis for the development of bacteriostatic biological agents and the control of mycotoxins.