The study aims to solve the problems of rapid deterioration of water environment and susceptibility to diseases in the sole breeding of largemouth black bass or yellow catfish, and to improve the aquaculture efficiency in this area. Based on the ecological niche difference between largemouth black bass and yellow catfish, the self-bred largemouth black bass fries with a specification of 71-83 g/tail and the purchased yellow catfish ‘Quanxiong No.2’ fries with a specification of 25-33 g/tail were used as experimental materials. By setting up double exquisite aquaculture of largemouth black bass and yellow catfish with different feeding densities, implementing targeted feeding strategies, implementing water quality management and disease prevention methods, the double exquisite aquaculture of largemouth black bass and yellow catfish was carried out and comparative experiment of nutritional effects was conducted. The results showed that bacterial hemorrhagic edema disease occurred in the No.3 pond where only yellow catfish were kept, with a survival rate of only 60%, while the survival rate of other experimental groups was above 80%. After about 160 days of breeding, the average sizes of largemouth black bass harvested from ponds 1, 2, 5, and 6 were 557.45g, 556.00 g, 565.33 g, and 571.31 g, with yields of 5663.25 kg, 5237.99 kg, 5359.46 kg, and 5469.22 kg, respectively. The average sizes of yellow catfish harvested from ponds 2, 5, 6, and 3 were 148.67 g, 162.10 g, 170.22 g, and 119.87 g, with yields of 883.64 kg, 727.58 kg, 547.53 kg, and 863.06 kg, respectively. According to the benefit analysis, the income of control pond No.1 is 203580 yuan/hm²; the control pond No.3 incurred a loss of 31507 yuan/hm²; the revenue of experiment pond No.2 is 217579 yuan/hm², with an increase of 13999 yuan/hm² compared to experiment pond No.1; the revenue of the experimental pond No.5 is 231221 yuan/hm², with an increase of 27641 yuan/hm² compared to the 1st pond; the revenue of the experimental pond No.6 is 233633 yuan/hm², with an increase of 30053 yuan/hm² compared to the 1st pond. It can be seen that double exquisite aquaculture has a better effect than single breeding. In pond breeding, it is recommended to release largemouth black bass with a density of about 27000 /hm² and yellow catfish with a density of 9000-13500 /hm² to achieve better benefits.