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Dynamics Analysis of Fatty Acids and Proteins in Industrial Hemp Seeds
CAIMengmeng, CUIHelu, WANGPan, ZHUHao, CHUPengfei, SUNXiaoxue, LIXuehai, WANGXiaonan
Journal of Agriculture ›› 2026, Vol. 16 ›› Issue (8) : 3-8.
PDF(1186 KB)
PDF(1186 KB)
Dynamics Analysis of Fatty Acids and Proteins in Industrial Hemp Seeds
The study aims to learn the dynamic changes of fatty acid and protein accumulation during the maturation process of industrial hemp seeds, and to explore the interaction of related enzymes, fatty acids, proteins and other related components during its development. Taking industrial hemp variety ‘Geliexiya’ as the research object, the seeds were sampled at different stages of 6 days (S1), 12 days (S2), 18 days (S3) and 21 days (S4) after flowering, and the moisture content, hundred-grain weight, fatty acid content, protein content, FAS activity and GS activity were determined and analyzed. The results showed that the moisture content of industrial hemp seeds increased in the period of S1-S2 and decreased in the period of S2-S4; the hundred-grain weight increased in the period of S1-S4; the fatty acid content decreased in the period of S1-S2 and increased in the period of S2-S4; the protein content decreased in the period of S1-S2 and increased in the period of S2-S4; the FAS and LOX were significantly negatively correlated with the fatty acid content; NR, GS, GDH, and GOGAT were highly significantly positively correlated with protein. Among them, FAS showed the highest correlation with fatty acid content, and GS showed the highest correlation with protein content. The conclusion is that fatty acids and proteins in industrial hemp seeds are synergistically accumulated in the middle and late stages of maturation, and FAS and GS are the core enzymes that regulate the accumulation of the two. This study provides a theoretical basis for the breeding of new varieties of high oil and high protein industrial hemp and the optimization of cultivation techniques. In the future, the molecular regulation mechanism can be further analyzed in combination with multi-omics technology.
industrial hemp / grain development / water content / 100 seeds weight / fatty acid content / protein content
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王小纯, 王晓航, 熊淑萍, 等. 不同供氮水平下小麦品种的氮效率差异及其氮代谢特征[J]. 中国农业科学, 2015, 48(13):2569-2579.
【目的】明确不同氮肥生理利用率小麦品种的氮代谢差异,为小麦高产及合理施肥提供理论依据,实现小麦节氮增产。【方法】采用大田试验方法,从16个小麦品种中筛选出氮素利用效率差异显著的低氮高效型小麦品种漯麦18、豫麦49-198和低氮低效型品种西农509、豫农202。然后进一步分析两类品种在N0(CK),N120(120 kg·hm<sup>-2</sup>)和N225(225 kg·hm<sup>-2</sup>)3个供氮水平下各小麦品种的产量、叶片GS活性、可溶性蛋白、游离氨基酸、NO<sub>3</sub><sup>-</sup>及全氮含量等氮代谢指标的差异。【结果】不同供氮水平下,氮肥生理利用率、产量、地上部及籽粒氮素积累量和叶片的GS活性、硝态氮含量、游离氨基酸含量、可溶性蛋白含量、全氮含量等均表现为低氮高效品种漯麦18、豫麦49-198显著高于低氮低效品种西农509、豫农202。增加供氮量,两类品种的产量、地上部及籽粒氮素积累量和叶片GS活性等氮代谢同化物指标均增加,而氮肥生理利用率降低。但两类品种对供氮水平响应不同,与N0相比,增加供氮量,低氮低效品种西农509、豫农202地上部及籽粒氮积累量、叶片的GS活性、硝态氮含量、游离氨基酸含量、可溶性蛋白含量、全氮含量的增幅均高于低氮高效品种漯麦18、豫麦49-198,但是,产量的增幅却显著低于低氮高效品种;氮肥生理利用率的降幅则以低氮高效品种显著高于低氮低效品种。【结论】低氮高效品种漯麦18、豫麦49-198相对于低氮低效品种西农509、豫农202具有更高的产量及氮素利用效率是因为其具有较高的GS活性,从而促进了植株对氮素的吸收与同化,使整个氮代谢过程利用效率提高,获得更高产量。低氮高效品种耐低氮能力较强,增产潜力较大;低氮低效品种对氮肥反应较为敏感,但是其氮素分配利用能力较低。
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