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Effects of Ustilaginoidea virens Infection on Structure and Diversity of Endophytic Fungal Communities in Rice
YUYanmin, LIUHaiying, XUZhenhua, GAODawei, ZHOUYuanhang, SONGAoran, WANGXin, YANPing
Journal of Agriculture ›› 2026, Vol. 16 ›› Issue (10) : 7-13.
PDF(4558 KB)
PDF(4558 KB)
Effects of Ustilaginoidea virens Infection on Structure and Diversity of Endophytic Fungal Communities in Rice
Rice false smut has become an important panicle disease that restricts the high quality and high yield of rice. The effect of pathogen infection on the community structure and diversity of endophytic fungi in rice is not clear, and it is difficult to support the exploration of endophytic biocontrol resources and the analysis of disease resistance mechanism. In order to investigate the effects of endophytic fungi on the resistance of rice plants to false smut of rice, the susceptible japonica rice variety ‘Songjing 15’ was used as the experimental material. Rice plants were artificially inoculated with conidiospores of Ustilaginoidea virens, and DNA was extracted from different tissues for construction of a fungal 16S rRNA gene library. High-throughput sequencing technology was used to analyze the structural composition and diversity of the fungal communities. The results showed that the diversity and richness of the endophytic fungal community in healthy plants were higher than that in infected plants. Species grouping belonged to 4 phyla, 15 classes, 26 orders, 44 families, and 56 genera. Infection by Ustilaginoidea virens altered the abundance of endophytic fungi in different rice tissues. At the genus level, compared with susceptible spikes, nine genera were increased and four genera were decreased in healthy spikes; eleven genera were increased and seven genera were decreased in healthy stems; eight genera were increased and six genera were decreased in healthy leaves; seven genera were increased and seven genera were decreased in healthy roots. Healthy plants had higher fungal community diversity and abundance than susceptible plants. Beneficial fungi such as Aureobasidium, Solicoccozyma, Phaeosphaeria, Coniothyrium, Nigrospora, Sporobolomyces, Nigrospora, Strobilurus, Pyrenochaetopsis with antibacterial activity and growth-promoting effects were found in higher abundance in healthy plants. This study provided a basis for biological control of rice false smut disease. Studies have confirmed that the infection of Ustilaginoidea virens can lead to the disorder of endophytic fungal community in rice and the decrease of beneficial fungal abundance.Healthy endophytic fungal structure is helpful to improve the disease resistance potential of rice. The results of this study provide a theoretical basis and resource support for the excavation of antagonistic endophytic fungi against rice false smut and the construction of biological control strategies for rice diseases. In the future, the separation and verification of dominant beneficial bacteria and the study of functional mechanism can be carried out.
rice false smut / rice / endophytic fungi / community structure / diversity / high-throughput sequencing technology / biological control
| [1] |
俞咪娜, 黄世文, 刘永峰. 稻曲病菌致病机制研究进展[J]. 植物病理学报, 2019, 49(6):721-729.
稻曲病是一种水稻穗部病害,在世界各水稻主产区均有发生,已成为世界性病害,在我国也属于水稻主要病害之一。稻曲病的发生严重影响水稻产量和稻米品质。研究其病原菌致病机制,对找寻防治药物的特异性靶标具有重要作用,可为抗病分子育种提供新的思路,为稻曲病菌防治药剂研发提供新的靶标。本文综述了稻曲病菌侵染过程、致病相关基因功能研究、水稻响应稻曲病菌侵染等方面的研究进展,提出进一步研究的策略。现有研究表明,稻曲病菌能成功侵染水稻孕穗期雄蕊的花丝引起发病。病原菌可通过抑制多个水稻免疫途径实现成功侵染;还可模仿胚珠受精激活水稻灌浆、糖代谢等途径为稻曲球的形成提供营养物质;全基因组测序完成和基因编辑技术在基因敲除中的成功应用,为稻曲病菌功能基因研究提供了很好的基础。稻曲病菌独特的侵染过程和营养利用机制是未来研究的重要方向。
|
| [2] |
高必达, 钟杰. 稻曲柄侵染过程研究进展[J]. 湖南农业科学, 2011(3):93-97.
|
| [3] |
|
| [4] |
祝浩文, 张亚玲. 黑龙江水稻稻曲柄病原菌生物学特性及其室内毒力测定[J]. 内蒙古农业大学学报, 2020, 41(6):16-21.
|
| [5] |
|
| [6] |
张俊喜, 成晓松, 宋益民, 等. 中国水稻稻曲病研究进展[J]. 江苏农业学报, 2016, 32(1):234-240.
|
| [7] |
|
| [8] |
姚琳, 叶慧丽, 胡容平, 等. 稻曲病侵染机制的初步研究[J]. 西南农业学报, 2012, 25(4):1273-1276.
|
| [9] |
李龚程, 张仕颖, 肖炜, 等. 水稻中内生菌研究进展[J]. 中国农学通报, 2015, 31(12):157-162.
为了给水稻与内生菌之间的作用机理研究提供参考，简述了水稻内生菌的种类，归纳了内生菌与水稻之间的相互作用，包括固氮作用、促生长作用、抗病作用等，探讨了内生菌在水稻体内的迁移效应。指出目前水稻内生菌的研究集中在功能性菌种的筛选与应用上，而没有系统地进行理论总结，目前关于水稻与内生菌相互作用机理仍然是一个谜团，应将纯培养与免培养的方法相结合，动态追踪水稻内生细菌的变化规律，并对未来水稻内生菌的研究趋势进行了展望。
|
| [10] |
姜晓宇, 高菊生, 徐凤花, 等. 水稻种子内生细菌多样性及其分泌植物生长激素能力的测定[J]. 微生物学报, 2013, 53(3):269-275.
|
| [11] |
|
| [12] |
赵娟, 刘涛, 潘磊, 等. 元阳梯田地方水稻品种根部内生菌及根际微生物的分离[J]. 应用生态学报, 2015, 26(12):3737-3745.
采用组织分离法和土壤稀释涂板法，对元阳哈尼梯田2个地方品种‘月亮谷’和‘红脚老粳’的根部内生细菌及根际土壤细菌进行了分离,研究元阳梯田传统水稻品种特殊的内生菌组成.结果表明: 试验共得到399个菌株.经形态特征及生理生化鉴定,月亮谷根部和其根际土壤分别分离到8和5个属,其中5个属是共有的;红脚老粳的根部和其根际土壤中分别分离到10和7个属,其中6个属是共有的.经分子生物学鉴定,月亮谷根部分离到11个种和5个属,根际土壤分离到8个种和4个属,其中5个种和4个属是共有的;红脚老粳根部分离到9个种和5个属,根际土壤分离到10个种和3个属,其中4个种和2个属是共有的.通过分子生物学鉴定，大部分菌株都可以鉴定到种,而通过形态及生理生化特性只能初步鉴定到属,但两种方法在属层次上的鉴定结果基本一致.元阳地方水稻根部内生细菌及根际土壤细菌具有一定的种属同源性与特异性.
|
| [13] |
|
| [14] |
|
| [15] |
|
| [16] |
张君成, 张炳欣, 陈志谊, 等. 稻曲病的接种方法及其效果初探[J]. 中国水稻科学, 2003, 17(4):390-392.
在水稻孕穗期于傍晚用注射法人工接种培养的稻曲病菌，发现病菌菌丝碎段与孢子混合可引起水稻严重发病，最高穗发病率可达100%，最多穗病粒数达106粒，最大病情指数达85.5。不同菌株引发的病情差异很大；病菌在不同品种上引发的病情也差异悬殊；病情指数与穗发病率之间及病情指数与穗平均病粒数之间存在高度相关。初步发现菌株品种之间存在专化互作现象。
|
| [17] |
In this study, endophytic bacteria isolated from root, stem, and leaf tissues of stripe rust-susceptible (Inqilab 91, Galaxy 2013, and 15BT023) and stripe rust-resistant (NARC 2011, Ujala 2015, TW1410) cultivars were identified and characterized. Abundance of endophytes was found in roots as compared with stems and leaves. Resistant and susceptible cultivars significantly differed in abundance of endophytic bacteria. Restriction analysis of 16S rRNA genes amplified from 100 bacterial isolates produced 17 unique patterns. Representatives of each of the 17 unique patterns were sequenced and identified. Among the sequenced bacteria, 8 belonged to Firmicutes, 7 were Proteobacteria, and 2 were Actinobacteria. Most of the isolates have plant growth-promoting properties and a few have the potential of producing hydrolytic enzymes. Two isolates showed significant inhibition of rust spore germination. These endophytic bacteria not only can be helpful in growth-promoting activities but also can assist in biocontrol of stripe rust disease.
|
| [18] |
|
| [19] |
沙月霞. 不同水稻组织内生细菌的群落多样性[J]. 微生物学报, 2018, 58(22):2216-2228.
|
| [20] |
杨娟, 董醇, 陈万浩, 等. 不同地区杜仲皮内生真菌群落组成及生态功能结构的差异分析[J]. 中国中药杂志, 2019, 44(6):1126-1134.
|
| [21] |
王国迪, 陈瑞, 汪彦欣, 等. 内生真菌稻镰状瓶霉对直播稻稻瘟病的防治效果[J]. 中国稻米, 2019, 25(4):68-73.
野生稻内生真菌稻镰状瓶霉在实验室条件下接种到水稻上，可以诱导水稻产生对稻瘟病的抗性。为了明确该内生菌田间不同时期施药对直播水稻稻瘟病的防治效果，2017年在浙江省桐庐县开展了田间试验。结果显示，稻镰状瓶霉不同时期施药对直播稻稻瘟病的防效与春雷霉素和芽孢杆菌相当，不同处理之间差异不显著。施用菌剂的处理对叶瘟的防效均达到70%以上；稻镰状瓶霉播后7 d、14 d施药处理对穗颈瘟的防效亦达70%以上。由此可见，稻镰状瓶霉可用于直播稻稻瘟病防治，拌种或播后撒施1次即可有效防治稻瘟病。
|
| [22] |
|
| [23] |
|
| [24] |
魏娜, 徐琼, 张宁, 等. 掷孢酵母及其应用研究进展[J]. 微生物学通报, 2014, 41(6):1211-1218.
|
| [25] |
Nine yeast strains capable of producing exopolysaccharides as determined by the phenol/sulfuric acid method were isolated and purified from dairy fan, a traditional fermented milk product in Yunan, China by the streak plate method. These strains were identified through morphological, physiological characteristics and molecular biology techniques, including 3 strains of Rhodotorula mucilaginosa, 1 strain of Candida albicans, 4 strains of Candida zeylanoides and 1 strain of Papiliotrema aurea. A high-yield exopolysaccharide (EPS)-producing strain, namely P. aureus DF-12, was obtained, which could synthesize EPS by using various carbon sources such as glucose, sucrose and molasses, and the highest yield of EPS of 3 510 mg/L was obtained after 168 h fermentation at 28 ℃ using sucrose as carbon source. In vitro antioxidant tests indicated that the EPS from DF-12 had strong scavenging ability against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion radicals. The experimental results provide a basis for the development and application of EPS in the food and pharmaceutical industries.
|
| [26] |
熊强强, 魏雪娇, 施翔, 等. 多层组学在植物逆境及育种中的研究进展[J]. 江西农业大学学报, 2018, 40(6):1197-1206.
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