Autotoxicity was a chemical interaction mechanism among intra-specific plant individuals. The aim f this study was to explore the relationship between intraspecific competition and population regeneration pproach after destroyed. Population of Eupatorium adenophorum (Spreng.) natural regeneration after frost damage and the effects of aqueous leaf extracts on seedling growth was studied by bioassay and field nvestigate. The results showed that the aqueous leaf extracts of Eupatorium adenophorum (Spreng.) could bviously promote the growth of seedlings, the promoting effect on seedling height increased at first and then gradually weakened, when the concentration was 3% the promote effect reached the peak; The seeds of Eupatorium adenophorum (Spreng.) in the wet autumn was easily germination when the area invaded by upatorium adenophorum (Spreng.) was break, no broken zone due to the litter coverage, hindered the seed germination, soil seed bank mainly distributed in 0-10 cm soil layer; Species update by tiller regeneration method when the dominant species ofEupatorium adenophorum (Spreng.) was damaged after frost. The results of this paper showed thatEupatorium adenophorum (Spreng.) had strong resistance capacity to autotoxicity, and its species update was by tiller regeneration method under the arid conditions.