Abstract: In order to study the relationship between fertilization level and yield of ‘Yongyou 12’, Reveal the single cropping rice super high yielding cultivation fertilizer needed regularity, improve the overall level of fertilization technology , Used in the unified cultivation technology measures of formula fertilization by soil testing, tracking survey 12 demonstration of different fertilizer combinations and the amount of fertilizer from 2012 to 2013 , to the expert group paid real cutting method to examine production, research on single cropping rice ‘Yongyou 12’fertilization and yield relationship and super high yield cultivation technique of fertilization. The results showed that, under moderate soil fertility levels, the yield (Y) of ‘Yongyou 12’ was accelerated with the increase of fertilizer application, according with the power function shown as Y=0.00003F2-0.2963F 11302 (Y=0.2301F 9258), Y=0.0504N2 24.566N 1953 (Y=51.243N-1543), Y=0.0361P2 26.596P 870 (Y=34.143P 8358), Y=0.0208K2 7.4996K 8499 (Y=17.793K 7306). Based on the function, the paper summarized a fertilizer combination for ‘Yongyou 12’ to obtain yield to 11000-12000 kg/hm2 was organic fertilizer 8000-12000 kg/hm2, nitrogen (N) 245-265 kg/hm2, phosphor (P2O5) 75-105 kg/hm2 and potassium (K2O) 210-265 kg/hm2. The structure of N, P, K ratio was 1.00:0.35:0.93. Another fertilizer combination for ‘Yongyou 12’ to obtain yield to 15000 kg/hm2 was organic fertilizer 15000-20000 kg/hm2, nitrogen (N) 320 kg/hm2, phosphor (P2O5) 190 kg/hm2 and potassium (K2O) 410 kg/hm2 with the structure of N, P, K ratio was 1.00:0.59:1.28, which production costs and risk increased remarkably. For living up to the expectations, a series of coordination measures were suggested including super cultivation, fertilizer application, water management and control of diseases and pets.