To investigate the biocontrol activity of Streptomyces corchorusii strain NF0919 and Bacillus subtilis DJ-6 WP to grape downy mildew, we had determined the indoor toxicity of the supernatant of S. corchorusii strain NF0919,1.0×1011cfu/g B.subtilis DJ-6 WP, mancozeb and dimethomorph on Plasmopara viticola by using leaf disc method, respectively,and the field control tests were conducted. The results showed that the EC50 values of the supernatant of strain NF0919,1.0×1011cfu/g B.subtilis DJ-6 WP, mancozeb and dimethomorph were 96.2859, 86.6038,69.9472 and 7.2636 μg/mL, respectively. The values of field control effect used in preventive experiments for grape downy mildew on the 7th day after 2 times spraying with 20 times liquid of the supernatant of strain NF0919 and 1000 times liquid of 1.0×1011cfu/g B.subtilis DJ-6 WP were 71.55% and 70.71%,the values of field control effect of 14th day after 2 times spraying were 67.54% and 68.19%, respectively. The values of field control effect used in curative experiments on the 7th day after 2 times spraying with 20 times liquid of the supernatant of strain NF0919 and 1000 times liquid of 1.0×1011cfu/g B.subtilis DJ-6 WP were 59.72% and 56.07%,the values of field control effect of 14th day after 2 times spraying were 56.88% and 57.46%, respectively. The field control effect of two kinds of supplied test biocontrol agents were equivalent.The protective effect between each of supplied test biocontrol agents and 300 times liquid of of 50%mancozeb WP had not significant difference, but there were significant difference at the efficacy between each of supplied test biocontrol agents and 200 times liquid of 40% dimethomorph SC. In conclusion, the S.corchorusii strain NF0919 and B.subtilis DJ-6 WP have certain biocontrol potential to grape downy mildew and value of development.
陈宏州 and
. Toxicity Test and Field Control Effect of 4 Different Fungicides on Grape Downy Mildew[J]. Journal of Agriculture, 2016
, 6(1)
: 37
-41
.
DOI: 10.11923/j.issn.2095-4050.cjas15060026
[1] 李海强. 石河子地区葡萄霜霉病的发生规律及防治研究[D]. 新疆: 石河子大学,2009.
[2] 王忠跃. 中国葡萄病虫害与综合防控技术[M]. 北京:中国农业出版社,2009.
[3] 张松强,王立如. 田间药剂防治葡萄霜霉病的效果[J]. 安徽农学通报,2007,13(12):169-170.
[4] Cadle-Davidson L. Variation within and between Vitis spp. for foliar resistance to the downy mildew pathogenplasmoparaviticola[J]. Plant disease,2008,92(11):1577-1584.
[5] 朱卫刚,胡伟群,刘燕君,等. 新型杀菌剂嘧菌酯对葡萄霜霉病室内生物活性评价[J]. 中国南方树, 2011,40(4):80-81.
[6] 李艳琼. 5种杀菌剂对葡萄霜霉病的控制效果[J]. 甘肃农业大学学报,2011,46(6):62-65.
[7] 毕秋艳,马志强,韩秀英,等. 葡萄霜霉病菌对甲霜灵抗药性治理及其田间抗药菌株遗传稳定性分析[J]. 植物病理学报,2014,44(3):302-308.
[8] Gisi U, Chin K M, Knapova G, et a1. Recent developments in elucidating modes of resistance to phenylamide, DMI and strobilurin fungicides[J]. Crop Protection,2000,19: 863-872.
[9] Benign M, Bompeix G. Post harvest control of Phytophthora cryptogea of witloof chicory with different fungi-cides and possible occurrence of resistant strains[J]. Crop Protection,2006,25(4):350-355.
[10] 罗彦平,王强,刘琳,等.新疆葡萄霜霉病对4种杀菌剂的敏感性研究[J]. 新疆农业科学,2013,50(5):851-856.
[11] 李宝燕,王英姿,刘学卿,等.3种杀菌剂对葡萄霜霉病菌的毒力测定和田间药效试验[J].江苏农业科学,2014,42(1)98-99.
[12] 杨敬辉,文平兰,陈宏州,等. 放线菌NF0919的鉴定及生防活性[J]. 西北农业学报,2013,22(4):201-206.
[13] 陈宏州,庄义庆,杨敬辉. 黄麻链霉菌NF0919菌株对草莓枯萎病菌的生防活性初探[J].江西农业学报,2014,26(11):54-57.
[14] Tooly P W, Sweigard J A, Fry w E, et a1. Fitness and virulence of Phytophthora infestans isolates from sexu-al populations[J]. Phytopathology,1986,76:1209-1212.
[15] 方中达. 植病研究方法[M]. 第3版. 北京:中国农业出版社,1998.
[16] 农业部农药检定所. 农药田间药效试验准则(二):杀菌剂防治葡萄霜霉病药效试验准则[M]. 北京:中国标准出版社,2000:234-238.
[17] 陈浩,胡梁斌,唐春平,等. 枯草芽孢杆菌B-FS01对葡萄霜霉病的防治效果[J]. 植物保护,2011,37(6):194-197.
[18] 岳宪化,胡夫防,段丽峰,等. 哈茨木霉菌防治葡萄霜霉病试验[J]. 中国果树,2014,(2):54-56.
[19] 扈进冬,吴远征,李纪顺,等. 拮抗性多粘类芽孢杆菌PB-2及其制剂对葡萄霜霉病的防效测定[J]. 山东农业科学,2014,27(3):30-33.
[20] 藏超群,赵奎华,刘长远,等. 生防菌株SY286对葡萄霜霉病菌的抑菌效果[J]. 沈阳农业大学学报,2014,45(2):221-224.
