The aim is to clarify the effects of low temperature at booting stage on seed- setting rate and physiological characteristics of leaves. This study was conducted with two rice cultivars‘Longjing11’(cold sensitive) and‘Longdao5’(cold tolerant) which were planted in pots. At booting stage, the low temperature treatments (15℃, lasting 1, 2, 3, 4, 5 d) were carried out in climatic laboratory. The effects of low temperature on leaf membrane permeability and antioxidant enzymes of the cultivars were discussed. The results showed that the seed-setting rate of‘Longdao5’had little change in the low temperature condition, and the seedsetting rate of‘Longjing11’decreased significantly. The activities of SOD, POD and CAT in leaves first showed an increasing trend then a decreasing trend. The activities of antioxidase, contents of soluble sugar and soluble protein of‘Longdao5’were higher than those of‘Longjing11’, but the content of malondialdehyde (MDA) and relative conductivity were lower than that of‘Longjing11’. It indicated that low temperature stress at booting stage could influence the physiological index such as membrane permeability and activities of antioxidant enzyme, and reduce the seed-setting rate.
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