The bulbs of Zantedeschia hybrida ‘Gaoyuan’ were used as explants to study the tissue culture and rapid propagation, screen the best culture medium for rapid propagation. The results showed that the best disinfectant time by 0.1% HgCl2 was 15 min. The best medium for callus induction was MS+0.5 mg/L 6-BA+0.1-0.2 mg/L NAA. Callus would grow into cluster buds, and the buds grew well with new callus proliferation in the medium of MS+2.0 mg/L 6-BA+0.2 mg/L NAA. In the medium of 1/2MS +0.2 mg/L NAA+10% banana, the rooting was 100%, and the roots grew thick, strong and thriving. Provide a reference for the system of rapid propagation and large-scale production on Zantedeschia hybrida.