The spatial association relationship of shale oil fractured reservoir is complicated, the seismic wave field characteristics of favorable fractures are complex, the seismic response characteristics are complex and diverse, and the seismic interpretation of shale oil fractured system is strong, which seriously affects the reliability of seismic identification of shale oil. Based on the geological, well logging and seismic data, this paper takes the Niuxie 55 well area of Jiyang Depression, Shengli Oilfield as an example. The fracture model of shale oil reservoir is established by comprehensive use of geological, logging and seismic data, by using the seismic forward modeling technology, the characteristics of fracture models with different orientations, angles and densities are analyzed, the seismic response mechanism of shale oil reservoir fractures is defined, and the shale oil fracture identification template is formed. The results show that the azimuth Angle has little effect on the seismic response characteristics of fractures. With the increase of fracture density, the intensity of seismic response and the degree of fracture fragmentation increase, and intermittent reflection and chaotic reflection appear. The reflection intensity of seismic response of fractures increases with the increase of inclination Angle. Low Angle fractures show weak reflection, high Angle fractures cause abnormal dithering of interlayer reflection, and vertical fractures show chaotic reflection. According to the established shale oil fracture identification template, improve the reliability of fracture identification, and lay a foundation for shale oil exploration and development.