F-53B as a unique perfluorinated and polyfluoroalkyl substance (PFAS) in China, is widely used in industry and released into the environment because of its excellent stability. Recent studies have shown that it has persistent pollution and biological toxicity, endangering human production and life. Based on the resistivity method, this paper takes porous media as the research object to monitor the dynamic migration process of F-53B in saturated homogeneous and heterogeneous media. The results showed that: (1) When the experimental equipment is fully saturated with pollutants, the difference between the resistivity value of each medium and the background value is at least 60 Ω·m, indicating that the resistivity method can monitor the dynamic migration process of F-53B in saturated porous media. (2) The resistivity of the coarse sand decreased from 389 Ω·m to 285 Ω·m by introducing F-53B into the coarse sand, and then increased to 404 Ω·m after washing with water. The overall resistivity curve was concave, similar to the changing images of medium sand and fine sand. However, the resistivity of soil increased from 95 Ω·m to 115 Ω·m, and the overall change showed a trend of rising first and then stable. (3) The comparative analysis of homogeneous and heterogeneous media showed that the porosity and composition of the medium are the factors affecting the migration of F-53B in the medium. The experimental results showed that the migration process of F-53B in saturated heterogeneous porous media can be effectively monitored by resistivity method which provides a theoretical basis for follow-up field work.