Due to the complex surface and underground geological conditions, the S/N ratio of the seismic records in the Kashgar structural belt of the Tarim basin is extremely low and the seismic imaging of the underground geological targets is very difficult, which affect the understanding of underground geology and restrict the process of oil and gas exploration in this area. After summarizing previous successful exploration experiences and shortcomings, a high-density beam seismic acquisition technology has been developed and achieved good application results. The near surface velocity model by tomographic inversion and the static correction have been improved after using single-sensor or small geophone-array receiving. The inline and crossline noises were evenly and adequately sampled and the S/N ratio of the pre-stack seismic data has been improved by 3D denoising, and finally the seismic imaging of the complex underground structures has been improved remarkably by high trace-density acquisition. Compared with the wide-line seismic acquisition, high-density beam seismic can better solve the problems of low S/N ratio of seismic record and difficult imaging of complex high-dip structures in the mountainous areas. Some favorable exploration targets have been discovered using the new seismic data, and an significant breakthrough in the Carboniferous-Permian carbonate rocks has been achieved after drilling the exploration well QT1, opening up a new era of oil and gas exploration in the southwestern depression of the Tarim Basin.