Using super sweet corn inbreeding lines S1, S2, S3 and hybrid Yue Tian No.3 as materials, the efficient regeneration systems were established. The research showed that the genotype and length of immature embryo of super sweet corn were the key factors influencing the embryogenic callus development, 0.5-1.2mm immature embryo were better for embryogenic callus inducing. contents of sugar and cytokinins in medium were the main factors for plantlet regeneration, low content of sugar( 20g/L) and 6-BA（2mg/L）or Zeatin (3 mg/L) are more fit to the regeneration, and the rate of regeneration could be increased 1.68 times when callus treated with ABA in sub-culture for 3 weeks. IBA could improve plantlet rooting, and the content of 3.0mg/L was the best.
Key words
Super Sweet Corn; Tissue Culture; Regeneration
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
1． Hodges TK, Kamo KK, Imbrie CW, Becwar MR. Genotype specificity of somatic embryogenesis and regeneration in maize. Bio/Technol, 1996, 4: 219-223.
2． Amstrong CL, Green CE. Establishment and maintenance of friable, embryogenic maize callus and the involvement of proline. Planta, 1985, 164:207-214.
3． Schlappi M, Hohn Y.. Competence of immature maize embryos for Agro bacterium tumefaciens transformation, Plant Cell, 1992, 4:7-16
4． Reborts D. R., Abscisic acid and mannitol promote early development, maturation and storage protein accumulation in somatic embryo of interior spruce. Plant Physiol., 1991,83:247-254.
5． Wu M-S(吴敏生) Huang J-Q（黄健秋, Wei Z-M（卫志明）. Establishment of highly efficient regeneration system of maize. Acta Phytophysiologica Sinica（植物生理学报）, 2001,27(6):489-494
6． YANG Y-S， JIAN Y-Y， CHEN Y-L。Comparison of culture procedures for regeneration of plants from protoplast-derives calluses of rice (Oryza sativa L.). Acta Agronomica Sinica, 2000,26(4): 490-496
7. MEI C-S (梅传生), TANG R-S（汤日圣）, ZHANG J-Y（张金渝, WU G-N（吴光南）. Abscisic acid regulation on the rate of plantlet regeneration from rice calli in vitro. J of Agri. Biotech.（农业生物技术学报）, 1994,2(1):976-99
