Using Agrobacterium tumefaciens Obtained transgenetic Japonica plant

doi:10.11924/j.issn.1000-6850.040541

Chin Agric Sci Bull ›› 2004, Vol. 20 ›› Issue (5) : 41-41. DOI: 10.11924/j.issn.1000-6850.040541

Liu Yongwei, Meng Qiaoxia, Dang Yongzhi, Meng Zhaohe, Li Chunguang, Liu Guoquan

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Abstract

The varieties are Kongyu131, Kenjiandao6 and Kenjiandao7 of Oryza stiva L subsup. Japonica. Agrobacterium tumefaciens infected maturate embryo of rice in order to induce callus.By this way, waxy gene can be transferred into accepted materials. After twice resistance selection, transgenetic seeding can be obtained in differentiation culture medium. Transgenetic plant is dyed by GUS and be identified by PCR, which can testify that exterior gene has been transferred into Japonica plant.

Key words

Rice;Agrobacterium tumefaciens;Waxy gene;Genetic transformation

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Liu Yongwei, Meng Qiaoxia, Dang Yongzhi, Meng Zhaohe, Li Chunguang, Liu Guoquan. Using Agrobacterium tumefaciens Obtained transgenetic Japonica plant[J]. Chinese Agricultural Science Bulletin. 2004, 20(5): 41-41 https://doi.org/10.11924/j.issn.1000-6850.040541

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