SRAP is a new technique for molecular marker .It is simplicity, reliability, moderate throughput ratio and facile sequencing of selected bands. This paper describes the SRAP principle, protocol. SRAP aimed for amplification of open reading frames(ORFs) by special primer set. The forward prime of 17 base amplified exons, the reverse primer of 18 base amplified introns and promoters. Since introns, promoters and spacers are variable among different individuals, this makes it feasible to generate polymorphism. The amplified DNA fragments are separated by denaturing acrylamide gels and detected by autoradiography. This technique has been used in genetics mapping, genetic diversity, gene tagging, comparative genomics and heterosis prediction. The prospect of the SRAP marker application in plant genetics and research were discussed.
Key words
Sequence-related；Amplified Polymorphism； Molecular marker； application
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References
1王得元，何晓明，王鸣. 蔬菜生物技术概论[B]. 北京：中国农业出版社，2002，236~295
2贾继增. 分子标记种质资源鉴定和分子标记育种[J]. 中国农业科学，1995，29(4)：1~10
3 朱振东，贾继增. 小麦SSR标记的发展及应用[J]. 遗传，2003，25(3)：355~360
4 G. Li, C.F.Quiros. Sequence-related amplified polymorphism(SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica[J]. Theor Appl Genet, 2001, 103:455~461
5林忠旭，张献龙，聂以春，贺道华，吴茂清. 棉花SRAP遗传连锁图构建[J].科学通报，2003，48(15)：1676~1679
6 Riaz A, Li G, Quresh Z, Swati MS, Quiros CF. Genetic diversity of oilseed Brassica napus inbred lines based on sequence-related amplified polymorphism and its relation to hybrid performance[J].Plant Breeding, 2001, 120(5)：411~415
7Ferriol M, Pico B, Nuez F. Genetic diversity of a germplasm collection of cucurbita pepo using SRAP and AFLP markers[J].Theor Appl Genet，2003，107(2)：271~282
8袁力行，傅骏骅，刘新芝，彭泽斌，张世煌，李新海，李连成. 利用分子标记预测玉米杂种优势的研究[J]. 中国农业科学，2000，33(6)：6~12
9Li G, Gao M, Yang B, Quiros C F. Gene for gene alignment between the Brassica and Arabidopsis genomes by direct transcriptome mapping[J].Theor Appl Genet，2003，107(1)：168~180
