Results of this experiment showed, for CCRI 45 growing in cultivated region in Zhoukou in eastern Henan Province the most suitable application N levels were 112.5~187.5kg/hm2, highest fiber cotton yields could reach 1259.45kg/hm2, production structure factors were reasonable, economic benefit is very good, net income increased 4 yuan compared with no N, the insect and pest occurred lightly. Output Value increased greatly. High fiber cotton yields were received at lower N levels, it’s outstanding advantage for CCRI 45 to turn low cost to high yield.