PCR-RFLP technique was used to analyze the relationship between the polymorphisms of IGFBP3 gene and beef performance in 24 Luxi cattles and 19 Jinnan cattles. The results showed that there were polymorphisms in this locus, but only AA and AB genotypes. The genotype frequencies were 0.875/0.125 and 0.895/0.105 respectively. A and B gene frequencies were 0.938/ 0.062 and 0.948/0.052 respectively. Correlations between IGFBP3 genotypes and 19 beef performances were analyzed, and other indexes of individuals with genotypes AB were higher than those of AA except carcass depth, daily gain, net meat percentage, dressing percentagein Luxi cattle, which had no significant difference; While other indexes of individuals with genotypes AA were higher than those of AB except dressing percentage, bone weight, backfat thickness, carcass chest depth, ham girth, ham width, ham length, loin meat thickness in Jinnan cattle, which were insignificant.
Key words
Luxi cattle;Jinnan cattle;IGFBP3 gene;PCR-RFLP;Beef performance
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
1胡倡华,许梓荣.胰岛素样生长因子结合蛋白研究进展,2000,(2):2~5
2Chung E R, Kim W T, Kim Y S, et al. PCR-SSCP genotype effects of growth prolactin and insulin-like growth factor-1 genes on milk yield in Korean cattle ( Hanwoo) [C] .Asian-Australian Journal of Animal Science, 2000,(S):223
3Ge W,Davis M E, Hines H C, et al. Association of a genetic marker with blood serum insulin-like growth factor concentration and growth traits in Angus cattle.Journal of Animal Science [J],2001,79:1757~1762
4孙维斌,陈宏,雷雪芹,等.IGFBP3基因多态性与秦川牛部分屠宰性状的相关性.遗传,2003,25(5):511~516
5Macilla H J, Zhang H M, De Nise S K. A novel polymorphism in the bovine insulin-like growth hormone factor binding protein-3(IGFBP3) gene [J],Animal Genetics,1997,28:375
