Comparison to Isolation of Mouse ES Cells With LIF Media and Cardiomyocyte Media Conditioned

doi:10.11924/j.issn.1000-6850.050330

Chin Agric Sci Bull ›› 2005, Vol. 21 ›› Issue (3) : 30-30. DOI: 10.11924/j.issn.1000-6850.050330

Liu Junping ,, Cao Hongguo , An Zhixing , Zhang Yong

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Abstract

By comparing the difference between LIF media conditioned and rat cardiomyocyte media conditioned during isolation and culture of mouse ES cell, suitable media is selected in the future. Mouse embryos of 3.5 d were collected, cocultured with mouse fetal fibroblasts as feeder layer. According to the difference of ES cell media, mouse embryos were divided into two groups, one with LIF media, other with cardiomyocyte media conditioned. Results showed that isolation rate of ICM with cardiomyocyte media conditioned was 76.30 %, LIF media 59.35 %, and difference between two media was significant (p＜0.01). Isolation of mouse ICM with LIF media was earlier than that with cardiomyocyte media conditioned, and difference between two medias were 11, 11, 12, 10 and 12 h respectively, mean difference was 11.2 h. Differentiation rate of ES cell masses of 48th h with cardiomyocyte media conditioned was 51.55 %, LIF media was 31.69 %, and difference between two medias was significant (p＜0.05). In addition, Normal karyotype rate of mouse ES cell with cardiomyocyte media conditioned 78.6 % was slightly higher than that with LIF media 76 %, but difference was not significant.

Key words

LIF media conditioned;Rat cardiomyocyte media conditioned;ES cells;Difference

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Liu Junping ,, Cao Hongguo , An Zhixing , Zhang Yong. Comparison to Isolation of Mouse ES Cells With LIF Media and Cardiomyocyte Media Conditioned[J]. Chinese Agricultural Science Bulletin. 2005, 21(3): 30-30 https://doi.org/10.11924/j.issn.1000-6850.050330

References

1Evans M J, Kaufman M H. Establishment in culture of pluripotential cells from mouse embryos. Nature,1981,292(9): 154~ 156
2Martin G R. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci US, 1981, 78(12): 7634~7638
3Metcalf D. The Unsolved Enigmas of Leukemia Inhibitory Factor. Stem Cells, 2003, 21: 5~14
4Song H, Lim H, Das S K, et al. Dysregulation of EGF family of growth factors and COX-2 in the uterus during the preattachment and attachment reactions of the blastocyst with the luminal epithelium correlates with implantation failure in LIF-deficient mice. Mol Endocrinol., 2000, 14: 1147~1161
5Vogiagis D, Salamonsen L A. The role of leukaemia inhibitory factor in the establishment of pregnancy. J Endocrinol.,1999,160: 181~190
6Sun H, Shi W K. Expression of LIF gene during early development of mouse embryo. J Exp Biol , 1998, 31:105~109
7 Fukada K, Korsching S, Towle M F. Tissue- specific and ontogenetic regulation of LIF protein levels determined by quantitative enzyme im- munoassay. Growth Factors, 1997,14:279~ 295
8Chesnokova V, Melmed S. Leukemia inhibitory factor mediates the hypothalamic pituitary adrenal axis response to inflammation. Endocrinology, 2000, 141: 4032~4040
9 Ancey C, Corbi P, Froger J, et al. Secretion of IL-6, IL-11 and LIF by human cardiomy -ocytes in primary culture. Cytokine, 2002, 18(4): 199~205
10Morel D S, Taupin J L, Potier M, et al. Renal synthesis of leukaemia inhibitory factor (LIF), under normal and inflammatory conditions. Cytokine, 2000, 12(3): 265~271
11Tofaris G K, Patterson P H, Jessen K R, et al. Denervated Schwann cells attract macrophages by secretion of leukemia inhibitory factor (LIF) and monocyte chemoattractant protein-1 in a process regulated by interleukin-6 and LIF. J Neurosci. , 2002, 22: 6696~6703
12Bonifati C, Mussi A, Dauria L, et al. Spontaneous release of leukemia inhibitory factor and oncostatin-M is increased in supernatants of short-term organ cultures from lesional psoriatic skin. Arch Dermatol Res., 1998, 290(1-2): 9~13
13Duval D, Reinhardt B, Kedinger C, et al. Role of suppressors of cytokine signaling (Socs) in leukemia inhibitory factor (LIF)–dependent embryonic stem cell survival. FASEB J., 2000, 14:1577~ 1584
14薛庆善. 体外培养的原理与技术. 北京: 科学出版社, 2003.517~525.
15韩嵘,柴桂萱,尚克刚.小鼠心肌细胞条件培养基对形成小鼠胚胎干细胞集落的影响.北京大学学报(自然科学版),1997, 33: 185~188
16Rossant J. Stem cells from the mammalian blastocyst. Stem Cells, 2001,19 (6):477~482
17Boeuf H, Merienne K, Jacquot S, et al. The Ribosomal S6 Kinases, cAMP- responsive Element-binding, and STAT3 Proteins Are Regulated by Different Leukemia Inhibitory Factor Signaling Pathways in Mouse Embryonic Stem Cells. J. Biol. Chemi., 2001, 276(49): 46204~45211
18Tofaris G K, Patterson P H, Jessen K R, et al. Leukemia inhibitory factor receptor is structurally related to the IL-6 signal transducer, gp130. EMBO J., 1991, 10: 2839~2848
19Hibi M, Nakajima K, Hirano T. IL-6 cytokine family and signal transduction: a model of the cytokine system. J. Mol. Med., 1996, 74:1~12
20Pitard V, Lorgeot V, Taupin J L, et al. The presence in human serum of a circulating soluble leukemia inhibitory factor receptor (sgp190) and its evolution during pregnancy. Eur Cytokine Netw., 1998, 9(4): 599~605
21Zhang J G, Zhang Y, Owczarek C M, et al. Identification and characterization of two distinct truncated forms of gp130 and a soluble form of leukemia inhibitory factor receptor alphachain in normal human urine and plasma. J Biol Chem., 1998, 273:10798~ 10805
22Stewart C L, Kaspar P, Brunet L J, et al. Blastocyst implant- tation depends on maternal expression of leukae- mia inhibitory factor. Nature, 1992, 359:76~79
23Escary J L, Perreau J, Duménil D, et al. Leukaemia inhibitory factor is necessary for maintenance of haematopoietic stem cells and thymocyte stimulation. Nature, 1993, 363:361~364
24Croy B A, Guilbert L J, Browne M A, et al. Characterization of cytokine production by the metrial gland and granulated metrial gland cells. J Reprod Immunol., 1991, 19(2):149~166
25Chen J R, Cheng J G, Shatzer T, et al. Leukemia inhibitory factor can substitute for nidatory estrogen and is essential to inducing a receptive uterus for implantation but is not essential for subsequent embryogenesis. Endocrinology, 2000, 141: 4365~4372