Construction of Recombinant Expression Plasmid pYELmleA of mleA Gene and Expression in Saccharomyces cerevisiae

Liu Yanlin, Jiang Sixin, He Xiuping, Li Hua, Zhang Borun

Chin Agric Sci Bull ›› 2005, Vol. 21 ›› Issue (4) : 48-48.

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Abbreviation (ISO4): Chin Agric Sci Bull      Editor in chief: Yulong YIN

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Chin Agric Sci Bull ›› 2005, Vol. 21 ›› Issue (4) : 48-48. DOI: 10.11924/j.issn.1000-6850.050448
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Construction of Recombinant Expression Plasmid pYELmleA of mleA Gene and Expression in Saccharomyces cerevisiae

  • Liu Yanlin, Jiang Sixin, He Xiuping, Li Hua, Zhang Borun
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Abstract

Malolactic enzyme is the function enzyme to turn L-malic acid into L-lactic acid during malolactic fermentation (MLF). In this paper, mleA gene encoding for malolactic enzyme from pLmleA, PGK1 promoter from plasmid pVC727-6 and ADH1 terminator from plasmid

Key words

Oenococcus oeni;Malo-lactic enzyme gene;Recombinant expression Plasmid;Saccharomyces cerevisiae

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Liu Yanlin, Jiang Sixin, He Xiuping, Li Hua, Zhang Borun. Construction of Recombinant Expression Plasmid pYELmleA of mleA Gene and Expression in Saccharomyces cerevisiae[J]. Chinese Agricultural Science Bulletin. 2005, 21(4): 48-48 https://doi.org/10.11924/j.issn.1000-6850.050448

References

1李华.现代葡萄酒工艺学[M].西安:陕西人民出版社,2000. 2 Pascal R G, Denis D, Bernard D, et al. Handbook of Enology[M]. London: John Wiley & Sons Ltd. 2000 3 Labarre C, Guzzo J, Cavin J F, Divies C. Cloning and characterization of the genes encoding malolactic enzyme and the ma

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