Identification and Differentiation into Cardiomyocytes of Goat Bone 
Marrow Mesenchymal Stem Cells in vitro

doi:10.11924/j.issn.1000-6850.050501

Chin Agric Sci Bull ›› 2005, Vol. 21 ›› Issue (5) : 1-1. DOI: 10.11924/j.issn.1000-6850.050501

Identification and Differentiation into Cardiomyocytes of Goat Bone Marrow Mesenchymal Stem Cells in vitro

Pang Quanhai,, Zhang Huiru, Hua Jinlian, Ma Yongjiang, Dou Zhongying

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Abstract

The Guanzhong dairy goats were used in our experiment to study the characteristics of their bone marrow mesenchymal stem cells (BM-MSCs), such as isolation, culture, and identification. The results indicate that there are excellent potentials for the goat mesenchymal-like stem cells derived from goat bone marrow to proliferate and to resist freezing and thawing repeatedly. The goat mesenchymal-like stem cells can express cellular surface markers CD34, CD44, CD105, CD106, CD 166, and TGF-α, but not CD14 and CD45. And after treated by 5-azacytidine in vitro, they express cellular surface marker of cardiac α-actin and Schiff’s stain positive.

Key words

Goats;MSCs;Differentiation Potential;Identification;Cardiomyocyte

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Pang Quanhai,, Zhang Huiru, Hua Jinlian, Ma Yongjiang, Dou Zhongying. Identification and Differentiation into Cardiomyocytes of Goat Bone Marrow Mesenchymal Stem Cells in vitro[J]. Chinese Agricultural Science Bulletin. 2005, 21(5): 1-1 https://doi.org/10.11924/j.issn.1000-6850.050501

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