The Study of Matic Cell Nucear Tranfer of Transgenin (t-PA) in Goat

doi:10.11924/j.issn.1000-6850.050601

Chin Agric Sci Bull ›› 2005, Vol. 21 ›› Issue (6) : 1-1. DOI: 10.11924/j.issn.1000-6850.050601

Liu Fengjun, Wang Yongsheng, Zhang Yong

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Abstract

The development of nuclear transfer embryos derived from fetal fibroblasts including t-PA gene and ovary granulose cells were compared. The cleavagetate （82.9%）and Morulae rate （55.9%）of ovary granulose cells weremore high than those of fetal fibroblasts(75.7%,48.7%). Blastocyst rate (19.7%) of ovary granulose cells was significantly higher than that of fetal fibroblasts (11.0%). The development of nuclear transfer embryos derived from fetal fibroblasts including t-PA gene in media DMEM/F12 including 0.5%FBS for starvation and including 10%FBS were compared. The cleavagetate(75.6%) of nuclear transfer embryos in media DMEM/F12 adding 0.5%FBS is not significantly defferenter than that (75.8%)in media DMEM/F12 adding10%FBS. Morular rate (46.5%)and blastocyst rate (11.0%)in media DMEM/F12 including 0.5%FBS were higher than those (39.3%, 9.0%)in media DMEM/F12 adding10%FBS （P＞0.05）. The blastocyst rate(9.1%) nuclear transfer embryos in media SOF adding 10% bFF was higher that(4.2% )10% FBS. The blastocyst rate(11.2%) nuclear transfer embryos in media SOF adding 10% bFF was higher that(5.5% )10% FBS. SOF was better than CR1 for the early development in nuclear transfer embryos.

Key words

Fibroblasts cells;Ovary granulose cells;SOFaa;CR1aa;Nuclear transfer embryos

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Liu Fengjun, Wang Yongsheng, Zhang Yong. The Study of Matic Cell Nucear Tranfer of Transgenin (t-PA) in Goat[J]. Chinese Agricultural Science Bulletin. 2005, 21(6): 1-1 https://doi.org/10.11924/j.issn.1000-6850.050601

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