he study of canine or pig transfer factor effects on proliferation of canine lymphocytes

doi:10.11924/j.issn.1000-6850.050626

Chin Agric Sci Bull ›› 2005, Vol. 21 ›› Issue (6) : 26-26. DOI: 10.11924/j.issn.1000-6850.050626

Kong Qingbo,, Cong Liyuan, Li Jia, Chang Jianjun Chen Dekun, Zhang Yanming

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Abstract

Pick-off blood with anticoagulant aseptically and the lymphocytes were isolated. Effects ofFCS，PBMC，PHA-P of different concentration on promote lymphocytes proliferation were tested by orthogonal test. On the base of the best level combination, different concentration canine or pig TF was investigated to detect its effect on the activation of lymphocytes by MTT essay. The result of experiment demonstrate that the best levels combination of canine lymphocyte, PHA-P, calf blood serum were 2.5×106 unit/ml，6.25μg/ml，10% respectively. Under this optimum cultural condition, the lymphocytes remarkable proliferated between 0.195~25mg/ml of canine and pig TF，but the best stimulate concentration of the canine and pig TF only or it with PHA-P is 1.56mg/ml. Furthermore, it is confirmed that lymphocytes developed by the canine and pig TF single was much better than they cooperated with PHA-P. The results confirm that the same or difficult race TF’s effect was obvious and provides a basis for further application on the difficult race TF preventing and curing canine’s virus disease.

Key words

Transfer Factor;Lymphocyte;Lymphocytes’ proliferation experiment;Canine;Pig

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Kong Qingbo,, Cong Liyuan, Li Jia, Chang Jianjun Chen Dekun, Zhang Yanming. he study of canine or pig transfer factor effects on proliferation of canine lymphocytes[J]. Chinese Agricultural Science Bulletin. 2005, 21(6): 26-26 https://doi.org/10.11924/j.issn.1000-6850.050626

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