This test required sterile soil and non-sterile soil in two soil samples, test separately for the two soil samples of three different treatment that is: The antagonistic bacterium Bacillus soaking liquid, soil all mixed soil and some soil for potted plants mixed. In this paper, A pot experiment to study Verticillium dahliae 2-70 antagonistic bacteria in the soil and in the colonization of cotton roots and rhizosphere colonization of the situation. The results showed that under laboratory conditions, antagonistic bacteria can 2-70 in non-sterilized soil and sterilized soil colonization, and colonization Tatsu quantity 106cfu/g soil around, after five months has managed to maintain a higher antibacterial activity, and strains in sterile soil in the quantity colonization than non-sterilized soil in quantity. Cotton roots by bacteria recovery, confirmed the antagonistic bacteria in the 2-70 designated cotton root colonization, number of 103cfu/g.