In order to provide the theoretic basis in bioremediation of chlorimuron-ethyl-contaminated soil, a chlorimuron-ethyl degradable strain was obtained from chlorimuron-ethyl-contaminated soil. L-6, a bacterium which was able to degrade chlorimuron-ethyl, was isolated from the soil of long term applied with chlorimuron-ethyl by enrichment culture. Based on morphology observation, physiological and biochemical characteristics, 16S rDNA sequence analysis, the strain L-6 was identified preliminarily asPseudomonas putida. The effect of initial concentration of chlorimuron-ethyl, inoculation amount, temperature and pH on degradation efficiencies was studied. The optimal degrading conditions were: initial concentration of chlorimuron-ethyl 200 mg/L, inoculation amount of 10%, pH 8.0, respectively, under the optimal conditions, the degrading efficiency could reach more than 80% after 4 days at 30℃. These results indicated that the strain L-6 had certain potential of degrading chlorimuron-ethyl.