Object: To prepare liposome of eugenol and evaluate its quality. Method: The orthogonal design was used to optimize the prescription and preparation technology of Eugenol liposome. The liposome of Eugenol was prepared with the film dispersion-supersonic method. The result of preparation was evaluated with the encapsulation rate which it was determined by UV spectrophotometry after extraction from Eugenol liposome. The appearances and the particle diameter of Eugenol liposome were examined with transmission electron microscopy. Results: The optimum prescription and preparation of Eugenol liposome were 1:6 (proportion of Cholesterin to Phosphatidylcholine), 1:5 (proportion of eugenol to Phosphatidylcholine), 40 min (ultrasonic time). The encapsulation rate of Eugenol liposome was 88.3%±3%. The particle diameter of Eugenol liposome was about 200nm and its appearance was uniformity globular vesicle.