The aims were to establish the technique system which was about the tissue culture of Aleurites moluccana, and then choose the media which was the most appropriate for calli induction and differentiation. The results showed that, the ratio of the cytokinin and the archusia was the primary factor that acts on the calli induction. TDZ inhibited the induction that from the calli to the axillary buds. The best media for calli induction was: 1/2MS+ZT 2 mg/L+NAA 0.5 mg/L+choline chloride 2 mg/L. The most suitable media for inducing axillary buds was 1/2MS+6-BA 1 mg/L +IBA 0.1 mg/L. The ratio of the cytokinin and the archusia was the important factor for calli induction.