Determination of Organic Acid in Tobacco Root by Ferric Hydroxamate Spectrophotometry

doi:10.11924/j.issn.1000-6850.casb15090136

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Chin Agric Sci Bull ›› 2016, Vol. 32 ›› Issue (9) : 194-199. DOI: 10.11924/j.issn.1000-6850.casb15090136

Determination of Organic Acid in Tobacco Root by Ferric Hydroxamate Spectrophotometry

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Abstract

In order to determine the amount of organic acids in root exudates, the authors used spectrophotometer measurement method to analyze the linear relationship between the acetic acid, malic acid and citric acid, and the orthogonal test design to determine the reaction condition of the optimal parameters and the optimal combination, thus to measure the amount of the organic acids of‘Nongda202’, NC89 and K326 in root exudates. The results showed that the optimal condition for the color reaction was as follows: 0.08 mol/L hydrochloric hydroxylamine, 0.4 mol/L DDC, 0.7 mol/L FeCl3, 50℃ , 30 min reaction time and 520 nm wavelength. The advantages of the established method included wide linear range, high accuracy, high recovery rate and low detection limit. Therefore, the colorimetric method of the ferric hydroxide, which was simple and easy to operate, was a good method to determine the total amount of organic acids in root exudates of tobacco.

Key words

organic acid; spectrophotometry; orthogonal design; tobacco

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Determination of Organic Acid in Tobacco Root by Ferric Hydroxamate Spectrophotometry[J]. Chinese Agricultural Science Bulletin. 2016, 32(9): 194-199 https://doi.org/10.11924/j.issn.1000-6850.casb15090136

References

[1]Bais HP, Loyola-Vargas VM, Flores HE, et al. Root-specific metabolism: the biology and biochemistry of underground organs [J]. In Vitro Cellular Developmental Biology-Plant, 2001, 37:730–741.
[2]Gleba D, Borisjuk NV, Borisjuk LG, et al. Use of plant roots for phytoremediation and molecular farming [J]. Proceedings of the National Academy of the Sciences of the United States of America, 1999, 96(11):5973-5977.
[3]张红娟,张在龙,庞志华,等.电位滴定法测岩心中水溶性有机酸的含量[J].科学技术与工程,2010,10(7):1744- 1746.ZHANG Hong-juan, ZHANG Zai-long, PANG Zhi-hua, et al. Potentiometric Titration Measuring Water-soluble Organic Acids in Rock Core[J]. Science Technology and Engineering, 2010,10(7):1744- 1746.
[4]吴晓红,马美华,李小华.电位滴定法测定柑橘类水果总有机酸的含量[J].中国科技信息,2011(7):88- 89.Wu Xiao-hong, Ma Mei-hua, Li Xiao-hua. Determination of Total Organic Acids in Citrus Fruits by Potential Titration[J]. China Science and Technology Information, 2011(7):88- 89.
[5]李曙红,柳利,刘彬.气相色谱法测定环己烷氧化液中一元有机酸含量[J]. 合成纤维工业,2011,34(2):65- 66.Li Shu-hong, Liu Li, Liu Bin. Gas chromatography determination of monoprotic organic acids content in cyclohexane oxidation liquid[J]. China Synthetic Fiber Industry,2011,34(2):65- 66.
[6]王平,周荣.高效液相色谱法测定植物根系分泌物中的有机酸[J].色谱,2006,24(3):239- 242.WANG Ping, ZHOU Rong. Determination of Organic Acids Exuded from Plant Roots by High Performance Liquid Chromatography[J]. Chinese Journal of Chromatography,2006,24(3):239- 242.
[7]陈向明,戴向平,董秀丽.高效液相色谱法测定银杏果中有机酸的含量[J].分析科学学报,2011,27(3):336- 338.CHEN Xiang-ming, DAI Xian-ping, DONG Xiu-li. Determination of Organic Acids in Ginkgo Fruit by High Performance Liquid Chromatography[J]. Journal of Analytical Science, 2011,27(3):336- 338.
[8]周晓明,卢春生,郭春苗,等.高效液相色谱法测定葡萄中有机酸的含量[J].新疆农业科学,200l,48(4): 65l- 654.ZHOU Xiao-ming, LU Chun-sheng, GUO Chun-miao, et al. Determination of Organic Acids Content in Grape by HPLC[J]. Xinjiang Agricultural Sciences,200l,48(4): 65l- 654.
[9]余成浩,彭成,余葱葱,等. 制川乌配伍白芍不同配伍比例对酯型生物碱含量的影响[J].成都中医药大学学报,2007,30(4):43-45.YU Cheng-hao,PENG Cheng,YU Cong-cong, et al. Effects on Content of the Ester Alkaloids of Radix Aconiti Preparata (RAP) Compatibility Radix Paeoniae Albae (RPA) [J]. Journal of Chengdu University of Traditional Chinese Medicine,2007,30(4):43-45.
[10]张蓓笑,林鹿,蒋龙飞,等.分光光度法测定酯和内酯含量的研究[J]. 安徽农业科学,2010,38(8):4399-4401.ZHANG Bei-xiao, LIN Lu, JIANG Long-fei, et al. Determination of Ester and Lactone Based on Spectrophotometry[J]. Journal of Anhui Agricultural Sciences,2010,38(8):4399-4401.
[11]张渝阳,王鑫,赵丽娟,等.分光光度法测定银杏萜内酯的含量[J].辽宁化工,2006,35(10):618-620.ZHANG Yu-yang, WANG Xin, ZHAO Li-juan, et al. Spectrophotometric Determination of Total Terpenoids in Ginkgo Leaves[J].Liaoning Chemical Industry,2006,35(10):618-620.
[12]陈兴荣,董光平,赖林,等.分光光度法测定阿司匹林肠溶片的含量[J]. 数理医药学杂志,2010,23(4) :478-479.Chen Xing-rong, DONG Guang-ping, LAI Lin, et al. Determination of Aspirin Enteric-coated Tablets by Spectrophotometry[J].Journal of Mathematical Medicine,2010,23(4) :478-479.
[13]贾文平,蒋华江,李芳,等.酯化分光光度法间接测定乙酰氯[J]. 台州学院学报,2010,23(4) :478-479.JIA Wen - ping, JIANG Hua - jiang, LI Fang. Detennination of Acetyl Choride Using Indirect Esterifled Spectrophotometry[J].Journal of Taizhou University,2010,23(4) :478-479.
[14]万伟国,徐麦玲,陆爱玲,等.两种全血胆碱酯酶活性测定方法的相关性比较[J]. 劳动医学,2000,17(4):211-212.WAN Wei-guo, XU Mai-ling, LU Ai-ling, et al. The Evaluation of the Correlation between Two Methods for Measuring the Activity of Cholinesterase[J]. Journal labour medicine,2000,17(4):211-212.
[15]He YC, Ma CL, Xu JH, Li Z.2011.A high-throughput screening strategy for nitrile-hydrolyzing enzymes based on ferric hydroxamate spectrophotometry. Appl.Microbiol.Biotechnol.89 (3) :817-823.
[16]Cetin G, Sungur S. A spectrophotometric method for the determination of lisinopril in tablet[J]. Reviews in Analytical Chemistry, 2006, 25(1):1.
[17]Kostic DA, Mitic SS, Miletic GZ. Kinetic determination of citric acid in artificial juice[J]. Chemia analityczna, 2005, 50(6):1099-1108.
[18]牛金刚,梁晓静,刘霞,等.分光光度法测定水溶液中的有机酸含量[J].应用化学,2010,27(3):342-346.NIU Jin-Gang , IANG Xiao-Jing , IU Xia, et al. Spectrophotometric Determination of Carboxylic Acids in Aqueous Solutions[J]. Chinese Journal of Applied Chemistry,2010,27(3):342-346.
[19]周劲松,马英华,于海成,等.高氯酸羟胺的制备及应用[J].化学推进剂与高分子材料,2002(2):1-15.ZHOU Jin-song, MA Ying-hua, YU Hai-cheng, et al. Preparation and application of hydroxylamine perchlorate [J].Chemical Propellants Polymeric Materials,2002(2):1-15.
[20]张首才,孙丽娟.二茂铁羟肟酸Cu(Ⅱ)配合物的合成[J].辽宁化工,2009,38(8) :510-511.ZHANG Shou-cai, SUN Li-juan. Synthesis of Cu(Ⅱ) Complex of Hydroxamic Acid Containing Ferrocenyl[J].Liaoning Chemical Industry,2009,38(8) :510-511.
[21]杨铁钊,杨志晓,林娟,等.不同烤烟基因型根际钾营养和根系特性研究[J].土壤学报,2009,46(4):646-651.Yang Tie-zhao, Yang Zhi-xiao, Lin Juan, et al. K nutrition in rhizosphere and characteristics of roots of flue-cured tobaccos different in geotype[J]. Acta Pedologica Sinica,2009,46(4):646-651.
[22]杨铁钊,杨志晓,聂红资,等.富钾基因型烤烟的钾积累及根系生理特性[J].作物学报,2009,35(3):535-540.YANG Tie-Zhao, YANG Zhi-Xiao, NIE Hong-Zi. Potassium Accumulation and Root Physiological Characteristics of Potassium-Enriched Flue-Cured Tobacco Genotypes[J]. Acta Agronomica Sinica,2009,35(3):535-540.