Optimized Extraction Condition of Lentinan and Its Antibacterial and Antioxidant Activity

doi:10.11924/j.issn.1000-6850.casb15110107

PDF(453 KB)

Chin Agric Sci Bull ›› 2016, Vol. 32 ›› Issue (11) : 39-42. DOI: 10.11924/j.issn.1000-6850.casb15110107

Optimized Extraction Condition of Lentinan and Its Antibacterial and Antioxidant Activity

张敏瑜,齐延林,杨弘华,严章雪 and 汪波

Author information +

History +

Abstract

To improve the extraction yield of lentinan and study its antioxidant and antibacterial activity, lentinan was extracted using hot water assisted by ultrasonic. The L9(33) orthogonal test was designed to optimized the extraction condition and the influencing factors were solid-liquid ratio, extraction temperature and ultrasonic time. The results showed that the optimum extraction condition was extracting using 90℃ water assisted by ultrasonic for 40 min with the solid-liquid ratio being 1:40. Under this condition, the extraction yield of lentinan was 6.47%. When extracting lentinan using 80℃ water assisted by ultrasonic for 20 min with the solid-liquid ratio being 1:40, the antibacterial activity was the strongest, the inhibition zone diameter of Escherichia coli was (9.95± 0.86) mm, and that of Bacillus subtilis was (8.73± 0.57) mm. When extracting lentinan using 90℃ water assisted by ultrasonic for 30 min with the solid-liquid ratio being 1:30, scavenging activity of hydroxyl radicals reached the maximum of 22.04%. When extracting lentinan using 90℃ water assisted by ultrasonic for 40 min with the solid- liquid ratio being 1:30, the antioxidant activity was the strongest. The influencing degree of the three factors was in an order of solid- liquid ratio ＞ extraction temperature ＞ ultrasonic time. The results indicated that the optimal extraction condition could improve the extraction yield of lentinan, and lentinan had antibacterial and antioxidant activity to some extent.

Key words

ultrasonic; orthogonal test; antioxidant activity; antibacterial activity

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

张敏瑜,齐延林,杨弘华,严章雪 and 汪波. Optimized Extraction Condition of Lentinan and Its Antibacterial and Antioxidant Activity[J]. Chinese Agricultural Science Bulletin. 2016, 32(11): 39-42 https://doi.org/10.11924/j.issn.1000-6850.casb15110107

References

[1] Chihara G, Maeda Y, Hamuro J, et al. Inhibition of mouse sarcoma-180 by Polysaccharides from Lentinus edodes(Berk.)sing. Nature, 1969, 222:687-688.
[2] 郑虹,叶秋焰.香菇多糖的药理及临床应用研究进展[J].海峡药学, 2006, 18(4): 150-154.
[3] Kupfahl C, Geginat G, Hof H. Lentinan has a stimulatory effect on innate and adaptive immunity against murine Listeria monocytogenes infection[J]. International Immunopharmacology,2006, 6(4): 686-696.
[4] 暴增海, 石桐磊. 食用菌的抑菌作用[J]. 北方园艺, 2012, 18: 174-175
[5] 孙娟, 郑朝辉,刘磊, 等. 4 种珍稀食用菌粗多糖的抗氧化活性研究[J]. 安徽农业大学学报, 2011, 38(3): 404-409。
[6] 李向阳, 胡一鸿.伟翔华, 等原木花菇与袋栽花菇中香菇多糖抗氧化和抑菌能力比较[J]. 湖南农业科学, 2009,11:129-132
[7] 钟广耀,林楠,王淑琴,等. 香菇多糖的抗氧化性能与抑菌作用研究[J], 食品科技,2007,7(6)：141-144
[8] 史琦云,郭玉蓉,陈德蓉. 食用菌多糖提取工艺研究[J]. 食品工业科技, 2004, 25(2):98-100
[9] 韦巍, 李雪华. 多糖的研究进展[J]. 国外医学药学分册, 2005,32(3): 179-184
[10] 李小雨,王振宇,王璐. 食用菌多糖的分离、结构及其生物活性的研究进展[J]. 中国农学通报, 2012, 28(12): 236-240
[11] 胡斌杰, 陈金锋, 王宫南. 超声波法与传统热水法提取灵芝多糖的比较研究[J]. 食品工业科技, 2007, 28(2):190-192
[12] 江发寿, 刘金荣, 但建明, 等. 沙枣多糖的超声提取及含量测定[J]. 西北药学杂志, 2002, 17(1):13-14
[13] 刘金荣, 江发寿, 但建明, 等. 独尾草多糖的超声提取及含量测定[J]. 中草药, 2002, 33(4):322-323
[14] Riera E, Golas Y, Blanco A, et al . Mass transfer enhancement in supercritical fluids extraction by means of power ultrasound[J]. Ultrasonics Sonochemistry, 2004, 11(3-4)：241-244
[15] 张磊. 食用菌多糖超声浸提工艺及控制系统的研究[D]. 南京: 南京林业大学, 2008
[16] 胡斌杰, 陈金锋, 王宫南.超声波法与传统热水法提取灵芝多糖的比较研究[J]. 食品工业科技, 2007, 28(2): 190-192
[17] 陈留勇, 孟宪军, 贾薇, 等. 黄桃水溶性多糖的抗肿瘤作用及清除自由基、提高免疫活性研究[J]. 食品科学, 2004, 25(2)：167-170
[18] 郭春梅, 武荣兰, 封顺, 等. 香青兰多糖的提取、测定及其对活性氧自由基的清除作用[J]. 食品发酵与工业, 2005, 31(3)：129-132
[19] 游丽君. 泥鳅蛋白抗氧化肽的分离纯化及抗疲劳、抗癌功效研究[D]. 广州：华南理工大学轻工与食品学院,2010
[20] 张嫚, 胡大坤. 香菇多糖对鸡常见病原菌的体外抑菌活性[J]. 江苏农业科学,2009,10(5)：219-220
[21]胡国元, 李超影, 陈默, 等. 香菇多糖和金针菇多糖的提取及其抑菌活性[J].武汉工程大学学报, 2013, 35(6): 30-35
[22] 陆娟, 武哲斌, 屈长青. 超声波辅助提取香菇多糖的研究[J]. 安徽农业科学2010, 38(33): 18625 -18626, 18663
[23] 念保义, 王铮敏,黄河宁. 超声提取、超滤分离香菇多糖工艺的研究[J]. 化学与生物工程,2004, 21(4):16-18
[24] 安啸, 陆珏婕, 杜琪珍. 香菇多糖结构分析和构效关系研究进展[J].食品与机械, 2007, 23(6): 122-125