A Comparative Study on Different Extraction Methods for Plant Chlorophyll

Chin Agric Sci Bull ›› 2016, Vol. 32 ›› Issue (27) : 177-180.

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Chin Agric Sci Bull ›› 2016, Vol. 32 ›› Issue (27) : 177-180. DOI: 10.11924/j.issn.1000-6850.casb16010102

A Comparative Study on Different Extraction Methods for Plant Chlorophyll

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Abstract

To explore the effect of different extraction solution to extract Boston ivy chlorophyll, the leaf chlorophyll of five leaf Boston ivy were extracted by four solution such as 80% acetone solution, 96% ethanol solution, 90% ethanol acetone mixed solution and 95% ethanol acetone mixed solution, respectively. The results showed that the chlorophyll solution absorption peak values and the extraction ability of different extraction solution are different with different plant chlorophyll extract solution, from big to small being 95% ethanol acetone mixed solution > 90% ethanol acetone mixed solution > 80% acetone solution > 96% ethanol solution. Compared with the grinding method, immersion method of extracting chlorophyll is more efficient. It can provide a certain reference function for accurate extraction and detection of plant chlorophyll.

Key words

Boston ivy leaf; Chlorophyll content; Extraction method; Comparison

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A Comparative Study on Different Extraction Methods for Plant Chlorophyll[J]. Chinese Agricultural Science Bulletin. 2016, 32(27): 177-180 https://doi.org/10.11924/j.issn.1000-6850.casb16010102

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