In order to explore the combined application amount of micro-fertilizer for high-quality and high-yield potato in the arid agricultural area of Longzhong, the effects of iron and zinc fertilizer on potato yield, quality and soil nutrient were studied. The two-factor random block design was adopted, with zinc and iron fertilizer as the test factors, and three fertilization levels were set for zinc and iron fertilizer, namely: zinc fertilizer (0, 20, 40 kg/hm²) and iron fertilizer (0, 10, 20 kg/hm²), a total of 9 treatments. The results showed that single application of iron and zinc fertilizer and their interaction had significant effects on potato yield, quality and soil available zinc and iron content (P<0.05). Among them, the yields of Fe2Zn0 and Fe0Zn2 increased by 15.0% and 19.5% compared with Fe0Zn0, and the yield of Fe2Zn2 increased by 21.5% compared with Fe0Zn0. Fe2Zn0 increased the iron content by 12.4% compared with Fe0Zn0, and the zinc and iron content of Fe0Zn2 increased by 10.8% and 9.8% compared with Fe0Zn0. The yield, starch, iron and zinc content of Fe2Zn2 were significantly increased by 21.5%, 3.4%, 17.3% and 13.6% compared with Fe0Zn0, respectively. In the 0-10 and 10-30 cm soil layers, the available iron content of Fe2Zn0 was significantly increased by 15.0% and 4.0% compared with Fe0Zn0, the available zinc content of Fe2Zn2 was significantly increased by 104.2% and 42.9% compared with Fe0Zn0. Considering comprehensively, under the conditions of this experiment, the single application rates of iron and zinc micro-fertilizer were 20 kg/hm² and 40 kg/hm², respectively, and the combined application rate of iron fertilizer was 20 kg/hm² and zinc fertilizer 40 kg/hm² had the best effect, which was conducive to improving potato quality and increasing yield.