Effects on Growth, Bioactive Substance Accumulation and Antioxidant Activity of Tartary Buckwheat Sprouts: Different Wavelengths of LEDs Light

CHENLili, LVPing, LVShaojie, FANGMingzhi, LIBin, HUYuegao, XUEXuzhang, KANGWenyi

Chin Agric Sci Bull ›› 2024, Vol. 40 ›› Issue (12) : 45-52.

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Chin Agric Sci Bull ›› 2024, Vol. 40 ›› Issue (12) : 45-52. DOI: 10.11924/j.issn.1000-6850.casb2023-0600

Effects on Growth, Bioactive Substance Accumulation and Antioxidant Activity of Tartary Buckwheat Sprouts: Different Wavelengths of LEDs Light

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Abstract

In order to investigate the effects of different wavelengths of LEDs light sources on the growth of tartary buckwheat sprouts, red (R), blue (B) and green (G) LEDs were used to culture the sprouts, with the darkness as a control. The indicators including morphological growth, bioactive matter accumulation, key gene expression involved in flavonoid synthesis, and antioxidant activity were determined, respectively. The results showed that the hypocotyls of tartary buckwheat sprouts under B were deep red and the hypocotyl elongation was strongly inhibited. The accumulation of anthocyanins, total flavonoids and total phenols under B were the highest, which were 7.18, 2.96 and 2.49 times as that of D, respectively. The hypocotyl of tartary buckwheat sprouts under R showed a light pink color. The accumulation of the above three bioactive substances under R was also significantly higher than that of the control, which was 2.56, 1.68 and 1.40 times, respectively. The hypocotyl elongation of the sprouts under G was similar to that of R, but the appearance was transparent and white. The accumulation of bioactive matters under G was not significantly different from that of the control. Both B and R strongly induced the expression of key genes involved in flavonoid biosynthesis pathway, and significantly increased the antioxidant activity of the hypocotyls of tartary buckwheat sprouts. In conclusion, red and blue LEDs could significantly affect the morphological growth of tartary buckwheat sprouts, and increase the accumulation of bioactive substances as well as the antioxidant capacity. Meanwhile B treatment was recommended due to the best performance.

Key words

red/blue/green LEDs light source / tartary buckwheat sprouts / bioactive substance / antioxidant activity / flavonoid synthesis gene expression

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CHEN Lili , LV Ping , LV Shaojie , et al . Effects on Growth, Bioactive Substance Accumulation and Antioxidant Activity of Tartary Buckwheat Sprouts: Different Wavelengths of LEDs Light[J]. Chinese Agricultural Science Bulletin. 2024, 40(12): 45-52 https://doi.org/10.11924/j.issn.1000-6850.casb2023-0600

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