Seed Germination and Seedling Growth Physiological Characteristics of Brassica napus Under Simulated Drought Stress

ZHUYuanjie, TANa, ZHAOWeiguo, ZHANGMengru, LIChengjun, HEMaochang

Chin Agric Sci Bull ›› 2025, Vol. 41 ›› Issue (6) : 44-52.

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Chin Agric Sci Bull ›› 2025, Vol. 41 ›› Issue (6) : 44-52. DOI: 10.11924/j.issn.1000-6850.casb2024-0224

Seed Germination and Seedling Growth Physiological Characteristics of Brassica napus Under Simulated Drought Stress

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Abstract

This study investigated the drought resistant of different lines of Brassica napus, which provided germplasm resources for the breeding of drought resistant rapeseed varieties. Eight different genetic background lines of Brassica napus were used as experimental materials to simulate drought stress treatment with different concentrations of polyethylene glycol 6000 (PEG-6000). The SOD activity, CAT activity and MDA content were measured by colorimetric method, potassium permanganate titration method, and thiobarbituric acid method, respectively. The germination ratio and plant height of eight rapeseed lines both decreased with the increase of drought stress degree. However, the germination rate of ‘G32-1’ was between 96% and 98%, and it was almost unaffected. The plant height inhibition degree of‘G763-2’was the lightest (29.1%). The SOD activity of ‘G7-1’ showed the most significant decrease, with a decrease of 23.59 U/kg. Overall, the trend of SOD activity in ‘G32-1’ was higher than that in ‘G7-1’. The CAT activity of ‘Qingza No.1’ was relatively higher (1.16-1.48 mg/g), but the CAT activity of ‘G32-1’ was relatively lower (0.88-1.30 mg/g). The MDA content of ‘G7-1’ and ‘G32-1’ was at a relatively low level, which MDA content was 0.55-0.83 μmol/g and 0.47-1.14 μmol/g, respectively. In total, ‘G32-1’ had the strongest drought resistance and could be used as an excellent germplasm resource for cultivating drought resistant varieties of rapeseed.

Key words

Brassica napus / drought stress / germination ratio / plant height / SOD / CAT / MDA / drought resistance

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ZHU Yuanjie , TA Na , ZHAO Weiguo , et al . Seed Germination and Seedling Growth Physiological Characteristics of Brassica napus Under Simulated Drought Stress[J]. Chinese Agricultural Science Bulletin. 2025, 41(6): 44-52 https://doi.org/10.11924/j.issn.1000-6850.casb2024-0224

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Effects of cadmium (Cd(2+)) on photosynthetic and antioxidant activities of maize (Zea mays L.) cultivars (3223 and 32D99) were investigated. Fourteen-day-old cultivar seedlings were exposed to different Cd concentrations [0, 0.3, 0.6 and 0.9mM Cd(NO(3))(2) x 4H(2)O] for 8 days. The results of chlorophyll fluorescence indicated that different levels of Cd affected photochemical efficiency in 3223 much more than that in 32D99. In parallel, the level of Cd at 0.9mM caused oxidative damage but did not indicate cessation of PSII activity of the cultivars; plant death was not observed at highly toxic Cd levels. Additionally, the increase in Cd concentration caused loss of chlorophylls and carotenoid and membrane damage in both cultivars, but greater membrane damage was observed in 3223 than in 32D99. Depending on Cd accumulation, a significant reduction in dry biomass was observed in both cultivars at all Cd concentrations. The accumulation of Cd was higher in roots than in leaves for both cultivars. Nevertheless, cultivar 3223 transferred more Cd from roots to leaves than did 32D99. On the other hand, our results suggest that there were similar responses in SOD, APX and GR activities with increasing Cd concentrations for both cultivars. However, POD activity significantly increased at highly toxic Cd levels in 32D99. This result may be regarded as an indication of better tolerance of the Z. mays L. cultivar 32D99 to Cd contamination.
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朱广龙, 马茵, 霍张丽, 等. 酸枣保护酶活性和膜脂过氧化产物对干旱胁迫的响应[J]. 中国野生植物资源, 2014, 33(3):5-10.
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张美俊, 乔治军, 杨武德, 等. 不同糜子品种对低氮胁迫的生物学响应[J]. 植物营养与肥料学报, 2014, 20(3):661-669.
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黄婵. 植物抗旱生理基础研究进展[J]. 农村实用技术, 2019(6):62-64.
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韩翠英. 干旱胁迫下四个功能基因的表达模式与不同品种小麦的抗旱相关性分析[D]. 杨凌: 西北农林科技大学, 2015:23-25.
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